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The map label for this gene is narH

Identifier: 41408717

GI number: 41408717

Start: 2952912

End: 2954528

Strand: Reverse

Name: narH

Synonym: MAP2619c

Alternate gene names: 41408717

Gene position: 2954528-2952912 (Counterclockwise)

Preceding gene: 41408718

Following gene: 41408716

Centisome position: 61.17

GC content: 66.36

Gene sequence:

>1617_bases
ATGAAGGTCATGGCGCAGCTGGCGATGGTGATGAACCTCGACAAGTGCATCGGCTGCCACACCTGCTCGGTGACCTGCAA
GCAGGCCTGGACCAACCGGTCGGGCACCGAATACGTGTGGTTCAACAACGTCGAAACCCGTCCGGGACAAGGCTATCCGC
GTACCTACGAGGACCAGGAGCGGTGGCGTGGCGGCTGGATCCGCGACAAGCGGGGCCGGCTGCGATTGCGTGACGGCGGC
CGTTTCCAAAAGCTGCTGCGCATCTTCGCCAATCCCAAGCTGCCGGTGATCGGCGACTACTACGAGCCGTGGACCTACGA
CTACGAGAACCTGACCACCGCACCCGCCGGTGACACCTTCCCGACTGCCGCGCCGAAAAGCCTGATCAGCGGCGAGCCGA
TGAAGGTGTCCTGGGGACCGAACTGGGACGACAACCTGGCCGGGTCGCCGGAGATCCTGGCCGAGGACCCGATCCTGAAG
AAGGTCAGCGAAGAGGTGAAACTCTCGCTCGAGCAGACCTTCATGTTCTACCTGCCCCGGATCTGCGAGCACTGCCTCAA
CCCGTCCTGTGTGGCCTCGTGCCCGTCGGGCGCGATGTACAAGCGCAGCGAGGACGGCATCGTGCTGGTCGATCAGGACC
GCTGCCGCGGCTGGCGGATGTGTGTGTCCGGATGTCCTTACAAGAAGGTGTATTTCAACCACAAGACGGGCAAGGCCGAG
AAGTGCACGCTGTGCTATCCGCGGATGGAGGTGGGGCTGCCCACCATCTGCTCGGAGACCTGCGTGGGCCGGCTGCGCTA
TCTGGGTGTGGTGCTCTACGACATGGACCGGGTACTCGAGGCGGCGTCGGTGGAAAACGACACCGACCTCTACCGGGCGC
AGTGCGATATCCTGTTGGACCCCAACGACACCGACGTCATCGCCGGCGCGCGGGCGCAGGGCATTCCCGACGAGTGGATC
GAGGCCGCGCAGCGTTCCCCGGTGTACGCGCTGATCCACACCTATCGGGTGGCGCTGCCGCTGCACCCGGAATACCGGAC
CATGCCGATGGTGTGGTACATTCCGCCGCTGTCGCCGGTGGTCGACGCGGTCAGCCGCGACGGCCACGACGGCGAGGACC
TGGGCAACCTGTTCGGCGCGCTGGACGCGCTGCGCATCCCGATGCAATACCTGGCCGAGCTGTTCACCGCCGGTGACACC
GCGGTGGTCGAGGGCGTGTTGCGGCGGCTGGCGGCGATGCGCTCCTACATGCGCGACATCAACCTCGGCCGCGAAACCCA
GCCGCACATCCCGCATTCGGTGGGCATGACCGAGGAACAGATCTACGAGATGTACCGGTTGCTGGCCATCGCGAAATACG
AAGAGCGCTATGTCATCCCGACGGCCTTCGCGCCGCAGGCCCGCGACCTCGAGCAGATGGGCTGCTCGTTGACCGGCGAC
GGCGGGCCGGGCATGTACGAGTCGGAGCCGGTGGCCGTGGAGACCTTCCACGCCCTCAAGCAGCGCGGCGGCCCCACCGA
GGCAGAGCCCGGCAGCACCCGGCCGCGGGTGAACCTGCTGAACTGGGACGGCGGCCAGGTGCCCGCCGGCATGTTCCCCG
AGGCGCAGCGGCGATGA

Upstream 100 bases:

>100_bases
GCCCAGCACGCGTTCGCGTTCAACTACCTGGGGCCGACCGGCAACCAGCGCGACGAGGTGACGATCGTCCGTCGTCGCAG
CCAAGAGGTGGTGTACTGAC

Downstream 100 bases:

>100_bases
GGCTGCTGTCGGCGCTGCGGGAGCGCTCCAGCAGCCGGGTGATGCAGGATCGCCTGGTGTGGCAGGCGGCGTCCCTGCTG
CTGGCCTACCCCGACGACGG

Product: NarH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MKVMAQLAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGQGYPRTYEDQERWRGGWIRDKRGRLRLRDGG
RFQKLLRIFANPKLPVIGDYYEPWTYDYENLTTAPAGDTFPTAAPKSLISGEPMKVSWGPNWDDNLAGSPEILAEDPILK
KVSEEVKLSLEQTFMFYLPRICEHCLNPSCVASCPSGAMYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAE
KCTLCYPRMEVGLPTICSETCVGRLRYLGVVLYDMDRVLEAASVENDTDLYRAQCDILLDPNDTDVIAGARAQGIPDEWI
EAAQRSPVYALIHTYRVALPLHPEYRTMPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALDALRIPMQYLAELFTAGDT
AVVEGVLRRLAAMRSYMRDINLGRETQPHIPHSVGMTEEQIYEMYRLLAIAKYEERYVIPTAFAPQARDLEQMGCSLTGD
GGPGMYESEPVAVETFHALKQRGGPTEAEPGSTRPRVNLLNWDGGQVPAGMFPEAQRR

Sequences:

>Translated_538_residues
MKVMAQLAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGQGYPRTYEDQERWRGGWIRDKRGRLRLRDGG
RFQKLLRIFANPKLPVIGDYYEPWTYDYENLTTAPAGDTFPTAAPKSLISGEPMKVSWGPNWDDNLAGSPEILAEDPILK
KVSEEVKLSLEQTFMFYLPRICEHCLNPSCVASCPSGAMYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAE
KCTLCYPRMEVGLPTICSETCVGRLRYLGVVLYDMDRVLEAASVENDTDLYRAQCDILLDPNDTDVIAGARAQGIPDEWI
EAAQRSPVYALIHTYRVALPLHPEYRTMPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALDALRIPMQYLAELFTAGDT
AVVEGVLRRLAAMRSYMRDINLGRETQPHIPHSVGMTEEQIYEMYRLLAIAKYEERYVIPTAFAPQARDLEQMGCSLTGD
GGPGMYESEPVAVETFHALKQRGGPTEAEPGSTRPRVNLLNWDGGQVPAGMFPEAQRR
>Mature_538_residues
MKVMAQLAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGQGYPRTYEDQERWRGGWIRDKRGRLRLRDGG
RFQKLLRIFANPKLPVIGDYYEPWTYDYENLTTAPAGDTFPTAAPKSLISGEPMKVSWGPNWDDNLAGSPEILAEDPILK
KVSEEVKLSLEQTFMFYLPRICEHCLNPSCVASCPSGAMYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAE
KCTLCYPRMEVGLPTICSETCVGRLRYLGVVLYDMDRVLEAASVENDTDLYRAQCDILLDPNDTDVIAGARAQGIPDEWI
EAAQRSPVYALIHTYRVALPLHPEYRTMPMVWYIPPLSPVVDAVSRDGHDGEDLGNLFGALDALRIPMQYLAELFTAGDT
AVVEGVLRRLAAMRSYMRDINLGRETQPHIPHSVGMTEEQIYEMYRLLAIAKYEERYVIPTAFAPQARDLEQMGCSLTGD
GGPGMYESEPVAVETFHALKQRGGPTEAEPGSTRPRVNLLNWDGGQVPAGMFPEAQRR

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=488, Percent_Identity=57.5819672131148, Blast_Score=593, Evalue=1e-170,
Organism=Escherichia coli, GI1787478, Length=491, Percent_Identity=57.0264765784114, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=37.1212121212121, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=37.1212121212121, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI226510944, Length=92, Percent_Identity=36.9565217391304, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=36.2745098039216, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787749, Length=80, Percent_Identity=36.25, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790326, Length=80, Percent_Identity=35, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI2367345, Length=86, Percent_Identity=33.7209302325581, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 60601; Mature: 60601

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMAQLAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGQGYPRTYEDQE
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHH
RWRGGWIRDKRGRLRLRDGGRFQKLLRIFANPKLPVIGDYYEPWTYDYENLTTAPAGDTF
HHCCCCEECCCCCEEECCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
PTAAPKSLISGEPMKVSWGPNWDDNLAGSPEILAEDPILKKVSEEVKLSLEQTFMFYLPR
CCCCCHHHHCCCCEEEECCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ICEHCLNPSCVASCPSGAMYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAE
HHHHHCCCHHHHCCCCCCCEEECCCCEEEEECHHCCHHHHHHCCCCCEEEEEECCCCCCC
KCTLCYPRMEVGLPTICSETCVGRLRYLGVVLYDMDRVLEAASVENDTDLYRAQCDILLD
EEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEEEEEC
PNDTDVIAGARAQGIPDEWIEAAQRSPVYALIHTYRVALPLHPEYRTMPMVWYIPPLSPV
CCCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEHHEEEEECCCCCCCCCEEEEECCHHHH
VDAVSRDGHDGEDLGNLFGALDALRIPMQYLAELFTAGDTAVVEGVLRRLAAMRSYMRDI
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NLGRETQPHIPHSVGMTEEQIYEMYRLLAIAKYEERYVIPTAFAPQARDLEQMGCSLTGD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHCCCEEECC
GGPGMYESEPVAVETFHALKQRGGPTEAEPGSTRPRVNLLNWDGGQVPAGMFPEAQRR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MKVMAQLAMVMNLDKCIGCHTCSVTCKQAWTNRSGTEYVWFNNVETRPGQGYPRTYEDQE
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHH
RWRGGWIRDKRGRLRLRDGGRFQKLLRIFANPKLPVIGDYYEPWTYDYENLTTAPAGDTF
HHCCCCEECCCCCEEECCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
PTAAPKSLISGEPMKVSWGPNWDDNLAGSPEILAEDPILKKVSEEVKLSLEQTFMFYLPR
CCCCCHHHHCCCCEEEECCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
ICEHCLNPSCVASCPSGAMYKRSEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGKAE
HHHHHCCCHHHHCCCCCCCEEECCCCEEEEECHHCCHHHHHHCCCCCEEEEEECCCCCCC
KCTLCYPRMEVGLPTICSETCVGRLRYLGVVLYDMDRVLEAASVENDTDLYRAQCDILLD
EEEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEEEEEC
PNDTDVIAGARAQGIPDEWIEAAQRSPVYALIHTYRVALPLHPEYRTMPMVWYIPPLSPV
CCCCCEEECCCCCCCCHHHHHHHHCCCCEEEEEHHEEEEECCCCCCCCCEEEEECCHHHH
VDAVSRDGHDGEDLGNLFGALDALRIPMQYLAELFTAGDTAVVEGVLRRLAAMRSYMRDI
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NLGRETQPHIPHSVGMTEEQIYEMYRLLAIAKYEERYVIPTAFAPQARDLEQMGCSLTGD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHCCCEEECC
GGPGMYESEPVAVETFHALKQRGGPTEAEPGSTRPRVNLLNWDGGQVPAGMFPEAQRR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]