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The map label for this gene is folP2

Identifier: 41408668

GI number: 41408668

Start: 2889122

End: 2890045

Strand: Reverse

Name: folP2

Synonym: MAP2570c

Alternate gene names: 41408668

Gene position: 2890045-2889122 (Counterclockwise)

Preceding gene: 41408669

Following gene: 41408667

Centisome position: 59.84

GC content: 72.19

Gene sequence:

>924_bases
GTGCGACCTGGCCCTGCGCGCCCTCGGCCGACCAGGCACCATAGACCCGTGCAATCGACGTTCTGCGGGCGTCCGGTCGC
CGGTGATCGCGCGCTGATCATGGCGATCATCAACCGCACCCCGGACTCGTTCTACGACCGGGGCGCCACCTTCAGCGACG
ACGCCGCCCGGGCGGCCGTGCACCGGGCGGTGGCCGAGGGCGCCGACGTCATCGACGTCGGCGGCGTCAAGGCCGGGCCC
GGCCAGGACGTCGACGCCGACACCGAGATCGCCCGGCTGGTGCCGTTCATCGAATGGCTGCGCGACGCCTACCCGGACCA
GCTGATCAGCGCCGACACCTGGCGTGCCGAGGTGGCCAAGGTGGCCTGCGCGGCCGGTGCGGACCTGATCAACGACACCT
GGGGCGGCGTCGACCCGGCGCTGCCGGAGGTGGCCGCGGAGTTCGGGGCGGGCCTGGTGTGCTCGCACACCGGCAACGCG
CTGCCGCGCACCCGCCCGTTCCGGGTGAGCTACGGAACCAGCACCCGCGGCGTGGTCGACGACGTGATCGCCCAGGTCAC
GGCGGCCGCCGAGCGGGCGGTCGGCTGCGGCGTGGCCCCCGACCGGGTGCTGATCGACCCCACCCACGACTTCGGCAAGA
ACACCTTCCACGGCCTGCTGTTGTTGCGGCACGTCGGCGATCTTGTTAACACCGGATGGCCCGTGCTGATGGCTTTGAGC
AACAAGGACTTCGTCGGGGAGACTCTGGGTGTGGAATTGACCGAGCGGCTGGAGGGAACGTTGGCGGCCACCGCGCTGGC
GGCGGCCGCCGGCGCCCGGATGTTTCGGGTGCACGAGGTCGCGCCCACCCGCCGGGTGCTGGAAATGGTGGCCTCGATCC
AGGGCACCCGCCCGCCCACGCGCACGGTGAGGGGGCTCGCATGA

Upstream 100 bases:

>100_bases
GACCCGCTGTACGTGCTGGCCGGCCGCGACGAGGGCTACGTGCCGTTCTACGACGAATACCCCGACGAGGTCGCCGAGGG
CAAGCGGCCCTAAGGCGGCG

Downstream 100 bases:

>100_bases
CGACGTCCGACCTGGTCGCCGGGGAGCTGGCGGGCGACGGGCTGCGCGACACCCGGCCCGGCGACACCTGGCTGGCCGAC
CGCAGCTGGAACCGCCCCGG

Product: FolP2

Products: diphosphate; dihydropteroate

Alternate protein names: DHPS 2; Dihydropteroate pyrophosphorylase 2 [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRPGPARPRPTRHHRPVQSTFCGRPVAGDRALIMAIINRTPDSFYDRGATFSDDAARAAVHRAVAEGADVIDVGGVKAGP
GQDVDADTEIARLVPFIEWLRDAYPDQLISADTWRAEVAKVACAAGADLINDTWGGVDPALPEVAAEFGAGLVCSHTGNA
LPRTRPFRVSYGTSTRGVVDDVIAQVTAAAERAVGCGVAPDRVLIDPTHDFGKNTFHGLLLLRHVGDLVNTGWPVLMALS
NKDFVGETLGVELTERLEGTLAATALAAAAGARMFRVHEVAPTRRVLEMVASIQGTRPPTRTVRGLA

Sequences:

>Translated_307_residues
MRPGPARPRPTRHHRPVQSTFCGRPVAGDRALIMAIINRTPDSFYDRGATFSDDAARAAVHRAVAEGADVIDVGGVKAGP
GQDVDADTEIARLVPFIEWLRDAYPDQLISADTWRAEVAKVACAAGADLINDTWGGVDPALPEVAAEFGAGLVCSHTGNA
LPRTRPFRVSYGTSTRGVVDDVIAQVTAAAERAVGCGVAPDRVLIDPTHDFGKNTFHGLLLLRHVGDLVNTGWPVLMALS
NKDFVGETLGVELTERLEGTLAATALAAAAGARMFRVHEVAPTRRVLEMVASIQGTRPPTRTVRGLA
>Mature_307_residues
MRPGPARPRPTRHHRPVQSTFCGRPVAGDRALIMAIINRTPDSFYDRGATFSDDAARAAVHRAVAEGADVIDVGGVKAGP
GQDVDADTEIARLVPFIEWLRDAYPDQLISADTWRAEVAKVACAAGADLINDTWGGVDPALPEVAAEFGAGLVCSHTGNA
LPRTRPFRVSYGTSTRGVVDDVIAQVTAAAERAVGCGVAPDRVLIDPTHDFGKNTFHGLLLLRHVGDLVNTGWPVLMALS
NKDFVGETLGVELTERLEGTLAATALAAAAGARMFRVHEVAPTRRVLEMVASIQGTRPPTRTVRGLA

Specific function: Has very low affinity for the DHPS substrate 6- hydroxymethyl-7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism [H]

COG id: COG0294

COG function: function code H; Dihydropteroate synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082224, Length=275, Percent_Identity=33.4545454545455, Blast_Score=132, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324073, Length=314, Percent_Identity=28.9808917197452, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000489 [H]

Pfam domain/function: PF00809 Pterin_bind [H]

EC number: 2.5.1.15

Molecular weight: Translated: 32578; Mature: 32578

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50972 PTERIN_BINDING ; PS00792 DHPS_1 ; PS00793 DHPS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPGPARPRPTRHHRPVQSTFCGRPVAGDRALIMAIINRTPDSFYDRGATFSDDAARAAV
CCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHH
HRAVAEGADVIDVGGVKAGPGQDVDADTEIARLVPFIEWLRDAYPDQLISADTWRAEVAK
HHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHH
VACAAGADLINDTWGGVDPALPEVAAEFGAGLVCSHTGNALPRTRPFRVSYGTSTRGVVD
HHHHHCCHHHHCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCCCHHHH
DVIAQVTAAAERAVGCGVAPDRVLIDPTHDFGKNTFHGLLLLRHVGDLVNTGWPVLMALS
HHHHHHHHHHHHHHCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEC
NKDFVGETLGVELTERLEGTLAATALAAAAGARMFRVHEVAPTRRVLEMVASIQGTRPPT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCH
RTVRGLA
HHHCCCC
>Mature Secondary Structure
MRPGPARPRPTRHHRPVQSTFCGRPVAGDRALIMAIINRTPDSFYDRGATFSDDAARAAV
CCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHH
HRAVAEGADVIDVGGVKAGPGQDVDADTEIARLVPFIEWLRDAYPDQLISADTWRAEVAK
HHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHH
VACAAGADLINDTWGGVDPALPEVAAEFGAGLVCSHTGNALPRTRPFRVSYGTSTRGVVD
HHHHHCCHHHHCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCCCHHHH
DVIAQVTAAAERAVGCGVAPDRVLIDPTHDFGKNTFHGLLLLRHVGDLVNTGWPVLMALS
HHHHHHHHHHHHHHCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEC
NKDFVGETLGVELTERLEGTLAATALAAAAGARMFRVHEVAPTRRVLEMVASIQGTRPPT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCH
RTVRGLA
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0015 {4-aminobenzoate}} [C]

Substrates: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate; 4-aminobenzoate

Specific reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

General reaction: Aryl group transfer [C]

Inhibitor: Sulfaguanidine; Sulfanilamide; Sulfathiazole; Sulfonamides [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]