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The map label for this gene is prfA

Identifier: 41408560

GI number: 41408560

Start: 2766596

End: 2767669

Strand: Reverse

Name: prfA

Synonym: MAP2462c

Alternate gene names: 41408560

Gene position: 2767669-2766596 (Counterclockwise)

Preceding gene: 41408561

Following gene: 41408559

Centisome position: 57.3

GC content: 69.18

Gene sequence:

>1074_bases
ATGACGCAGCCGGTTCAGACCATCGACGTGCTGCTGGCCGAACACGCCGACCTGGAACGCCGGCTGTCCGACCCCGACCT
GCACAGCAATCCCGACGAGGCCCGCAAGGCCGGGCGGCGGTTCGCCCGGCTCGCCCCGATCGTCGGCACCTACCGCAAGC
TGATGGCCGCCCGCGAGGACCTCGAGACCGCGCGCGAACTTGCCGCCGACGACGATTCGTTCACCGCCGAGGTCGCCGAC
CTGGAATCCCGGGTGGCCCAGTTGGACACCCAGCTGACCGACATGCTGGCCCCGCGCGACCCGCACGACGCCGACGACAT
CGTGCTCGAGGTCAAATCCGGCGAAGGCGGCGAAGAGTCGGCGTTGTTCGCCGCCGACCTGGCCCGGATGTACATCCGCT
ACGCCGAGCGGCACGGCTGGACGGTCACGGTGCTGGACGAGATCACCTCGGACCTGGGCGGTTACAAGGACGCGACGCTG
TCCATCCGCAGCAAGGGCGACAGCGCCGACGGGGTGTGGTCGCGGATGAAGTTCGAGGGCGGGGTGCACCGGGTGCAGCG
CGTCCCGGTCACGGAATCCCAAGGCCGCGTGCACACTTCGGCGGCGGGTGTGCTCGTCTATCCCGAACCCGAAGAGGTCG
CCGAGGTGCAGATCGACGAGTCCGATCTGCGCATCGATGTGTTCCGCTCGTCGGGCAAGGGCGGGCAGGGCGTCAACACC
ACGGACTCCGCGGTGCGGATCACCCACCTGCCCACCGGCGTCGTGGTCACCTGCCAGAACGAGCGCTCGCAGCTGCAGAA
CAAGGCCCGCGCGCTGCAGGTGCTGGCGGCCCGGCTGCAGGCCATGGCCGAGGAGCAGGCCTCGGCCGAGGCGTCGGCCG
ATCGGGCCAGCCAGATTCGCACCGTCGACCGCAGCGAACGCATTCGCACCTACAACTTTCCGGAGAACCGGATCACCGAC
CACCGCATCGGCTTCAAGGCGCACAACCTCGACCAGGTGCTCGACGGCGACCTGGACGCGTTGTTCGACGCGCTGGCCGC
CGCCGACAAGCAGTCGCGGTTACAGCAGGCATGA

Upstream 100 bases:

>100_bases
ATAGCTCGGTTCCCGACGCCCGAACTGTGCGCGCAGTCGCACAGGCCGGGCGTCGGTTCACGTTTGAGGTCGTGGCGCGG
GGCAGGGCAGGAGGTGTGAG

Downstream 100 bases:

>100_bases
CCTCCCGGCGACGATGCACACGATGAGCAGCGAGACGATCCGGCTCAGCCTGCGGCGCGCGATCGACGACGCGACGGCCA
CCCTCGCCGAAGCCGGAATC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTQPVQTIDVLLAEHADLERRLSDPDLHSNPDEARKAGRRFARLAPIVGTYRKLMAAREDLETARELAADDDSFTAEVAD
LESRVAQLDTQLTDMLAPRDPHDADDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWTVTVLDEITSDLGGYKDATL
SIRSKGDSADGVWSRMKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVAEVQIDESDLRIDVFRSSGKGGQGVNT
TDSAVRITHLPTGVVVTCQNERSQLQNKARALQVLAARLQAMAEEQASAEASADRASQIRTVDRSERIRTYNFPENRITD
HRIGFKAHNLDQVLDGDLDALFDALAAADKQSRLQQA

Sequences:

>Translated_357_residues
MTQPVQTIDVLLAEHADLERRLSDPDLHSNPDEARKAGRRFARLAPIVGTYRKLMAAREDLETARELAADDDSFTAEVAD
LESRVAQLDTQLTDMLAPRDPHDADDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWTVTVLDEITSDLGGYKDATL
SIRSKGDSADGVWSRMKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVAEVQIDESDLRIDVFRSSGKGGQGVNT
TDSAVRITHLPTGVVVTCQNERSQLQNKARALQVLAARLQAMAEEQASAEASADRASQIRTVDRSERIRTYNFPENRITD
HRIGFKAHNLDQVLDGDLDALFDALAAADKQSRLQQA
>Mature_356_residues
TQPVQTIDVLLAEHADLERRLSDPDLHSNPDEARKAGRRFARLAPIVGTYRKLMAAREDLETARELAADDDSFTAEVADL
ESRVAQLDTQLTDMLAPRDPHDADDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWTVTVLDEITSDLGGYKDATLS
IRSKGDSADGVWSRMKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVAEVQIDESDLRIDVFRSSGKGGQGVNTT
DSAVRITHLPTGVVVTCQNERSQLQNKARALQVLAARLQAMAEEQASAEASADRASQIRTVDRSERIRTYNFPENRITDH
RIGFKAHNLDQVLDGDLDALFDALAAADKQSRLQQA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=279, Percent_Identity=48.3870967741936, Blast_Score=247, Evalue=9e-66,
Organism=Homo sapiens, GI34577120, Length=340, Percent_Identity=38.2352941176471, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI166795305, Length=176, Percent_Identity=48.8636363636364, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1787462, Length=348, Percent_Identity=45.9770114942529, Blast_Score=280, Evalue=8e-77,
Organism=Escherichia coli, GI2367172, Length=331, Percent_Identity=38.0664652567976, Blast_Score=207, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=252, Percent_Identity=39.6825396825397, Blast_Score=161, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6321295, Length=357, Percent_Identity=37.8151260504202, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI19921226, Length=330, Percent_Identity=38.4848484848485, Blast_Score=213, Evalue=1e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39314; Mature: 39183

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPVQTIDVLLAEHADLERRLSDPDLHSNPDEARKAGRRFARLAPIVGTYRKLMAARED
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETARELAADDDSFTAEVADLESRVAQLDTQLTDMLAPRDPHDADDIVLEVKSGEGGEES
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHH
ALFAADLARMYIRYAERHGWTVTVLDEITSDLGGYKDATLSIRSKGDSADGVWSRMKFEG
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCC
GVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVAEVQIDESDLRIDVFRSSGKGGQGVNT
CHHHHHCCCCCCCCCCEEECCCEEEEECCCHHHHEEEECCCCCEEEEEECCCCCCCCCCC
TDSAVRITHLPTGVVVTCQNERSQLQNKARALQVLAARLQAMAEEQASAEASADRASQIR
CCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVDRSERIRTYNFPENRITDHRIGFKAHNLDQVLDGDLDALFDALAAADKQSRLQQA
HHCHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQPVQTIDVLLAEHADLERRLSDPDLHSNPDEARKAGRRFARLAPIVGTYRKLMAARED
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETARELAADDDSFTAEVADLESRVAQLDTQLTDMLAPRDPHDADDIVLEVKSGEGGEES
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHH
ALFAADLARMYIRYAERHGWTVTVLDEITSDLGGYKDATLSIRSKGDSADGVWSRMKFEG
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCC
GVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVAEVQIDESDLRIDVFRSSGKGGQGVNT
CHHHHHCCCCCCCCCCEEECCCEEEEECCCHHHHEEEECCCCCEEEEEECCCCCCCCCCC
TDSAVRITHLPTGVVVTCQNERSQLQNKARALQVLAARLQAMAEEQASAEASADRASQIR
CCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVDRSERIRTYNFPENRITDHRIGFKAHNLDQVLDGDLDALFDALAAADKQSRLQQA
HHCHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA