Click here to switch to the map view.

The map label for this gene is folC

Identifier: 41408368

GI number: 41408368

Start: 2534928

End: 2536385

Strand: Reverse

Name: folC

Synonym: MAP2270c

Alternate gene names: 41408368

Gene position: 2536385-2534928 (Counterclockwise)

Preceding gene: 41408369

Following gene: 41408367

Centisome position: 52.52

GC content: 71.88

Gene sequence:

>1458_bases
ATGAGCGAGCCGCCCGACTGGGCGCCGGAGCACGACGTTCCCGACCCGGTCCCCACCCCGGACGAGATCGCCGCGCTGCT
GCAGGTCGAACACCTGCTGGACCAGCGCTGGCCGGAGACCAAGATCGAGCCCAGCCTGACCCGGATCAGCGCACTGCTGG
ACCTGTTGGGCTCGCCGCAGCGCGCCTACCCGTCCATCCACGTCGCGGGCACCAACGGCAAGACCTCGGTGGCCCGGATG
ATCGACGCGCTGGTCACCGCGCTGCACCGGCGCACCGGGCGCACCACCAGCCCGCACCTGCAATCCGCGGTGGAGCGCAT
CTCGATCGACGCCAAGCCGATCAGCCCGGCCCGGTATGTCGACACCTACCGCGAAATCGAGCCGTTCGTGCAGATGGTCG
ATCAGCAATCGCAAGCCGCCGGGGGACCGGCGATGAGCAAGTTCGAGGTGCTCACCGCGATGGCGTTCGCCGCGTTCGCC
GACGCCCCGGTCGACGTCGCCGTGGTCGAGGTGGGCATGGGGGGACGCTGGGACGCCACCAACGTGGTCAACGCCCCGGT
CGCGGTCATCACCCCGATCTCCATCGACCACGTCGACTACCTGGGCCACGATCTCGCCGGGATCGCCGGGGAGAAGGCCG
GCATCATCACCCGGGCGCCCGCGGGGGCACCCGACACCGTCGCGGTCATCGCCCAGCAGCCGCCGGAGGTGATGGAGGTG
CTGTTGGCGCAGTGCGTGCAGGCCGACGCCGCGGTGGCCCGCGCGGGCTCGGAGTTCGCGGTGCTGGGCCGGCAGGTCGC
GATCGGCGGGCAGCTGCTGCAGCTGCAGGGGCTGGGCGGGGTGTATTCCGACGTCTACCTGCCCCTGCACGGCGAGCACC
AGGCGCACAACGCCGCGCTGGCCCTGGCGGCGGTCGAGGCGTTCTTCGGCGCCGGCGCCCAGCGCCAGCTCGACGTCGAC
GCCGTTCGTGCCGGTTTCGCCGCCGTCACAAGCCCCGGCCGGCTTGAGCGAATGCGCAGCGCCCCAACGGTTTTCATCGA
CGCCGCGCACAATCCCGCCGGCGCGGCGGCACTGGCGCGCACGCTGGCCGACGAATTCGACTTCCGCTTCCTGGTCGGCG
TGCTCAGCGTCCTCGCCGACAAGGACGTCGACGGCATCCTGGCCGCGCTGCAGCCGGTGTTCCACTCCGTGGTGGTCACC
CACAACGGGTCCCCGCGGGCGCTCGACGTCGAGTCGCTGGCCCTGGCGGCGCGCGAGCAGTTCGGCCCCGATCGGGTGAG
CACCGCCGAGAACCTCAGCGACGCCATCGACGTCGCGACCGCGCTGGTCGACGACGCCGTCCGGGACGGGTTGGTGGACG
GGCAGGCCGAGGACTTCGCCGGGACCGGGATCGTCATCACCGGCTCGGTGGTCACCGCCGGCGCGGCGCGAACCCTGTTC
GGTCGTGATCCGCAATGA

Upstream 100 bases:

>100_bases
TGGCCAAGGCGCCCCCGCACGTCGTCGACAAGATCCGGGACCGACAGCGGCTGGCGCAGGAGGAAAGCGAGCGGATCAAC
GCGCGGCTGGCCGTGCTGCA

Downstream 100 bases:

>100_bases
CCCGGCCCGACCCACCCGAAGGCCCGCAGCAGCGCGGCGCGGCCGACCCGTGGCGCAGCTTCGGCGCGGTGATGGCCGCC
ACCCTGATCCTGGAGGCCAT

Product: hypothetical protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSEPPDWAPEHDVPDPVPTPDEIAALLQVEHLLDQRWPETKIEPSLTRISALLDLLGSPQRAYPSIHVAGTNGKTSVARM
IDALVTALHRRTGRTTSPHLQSAVERISIDAKPISPARYVDTYREIEPFVQMVDQQSQAAGGPAMSKFEVLTAMAFAAFA
DAPVDVAVVEVGMGGRWDATNVVNAPVAVITPISIDHVDYLGHDLAGIAGEKAGIITRAPAGAPDTVAVIAQQPPEVMEV
LLAQCVQADAAVARAGSEFAVLGRQVAIGGQLLQLQGLGGVYSDVYLPLHGEHQAHNAALALAAVEAFFGAGAQRQLDVD
AVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGAAALARTLADEFDFRFLVGVLSVLADKDVDGILAALQPVFHSVVVT
HNGSPRALDVESLALAAREQFGPDRVSTAENLSDAIDVATALVDDAVRDGLVDGQAEDFAGTGIVITGSVVTAGAARTLF
GRDPQ

Sequences:

>Translated_485_residues
MSEPPDWAPEHDVPDPVPTPDEIAALLQVEHLLDQRWPETKIEPSLTRISALLDLLGSPQRAYPSIHVAGTNGKTSVARM
IDALVTALHRRTGRTTSPHLQSAVERISIDAKPISPARYVDTYREIEPFVQMVDQQSQAAGGPAMSKFEVLTAMAFAAFA
DAPVDVAVVEVGMGGRWDATNVVNAPVAVITPISIDHVDYLGHDLAGIAGEKAGIITRAPAGAPDTVAVIAQQPPEVMEV
LLAQCVQADAAVARAGSEFAVLGRQVAIGGQLLQLQGLGGVYSDVYLPLHGEHQAHNAALALAAVEAFFGAGAQRQLDVD
AVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGAAALARTLADEFDFRFLVGVLSVLADKDVDGILAALQPVFHSVVVT
HNGSPRALDVESLALAAREQFGPDRVSTAENLSDAIDVATALVDDAVRDGLVDGQAEDFAGTGIVITGSVVTAGAARTLF
GRDPQ
>Mature_484_residues
SEPPDWAPEHDVPDPVPTPDEIAALLQVEHLLDQRWPETKIEPSLTRISALLDLLGSPQRAYPSIHVAGTNGKTSVARMI
DALVTALHRRTGRTTSPHLQSAVERISIDAKPISPARYVDTYREIEPFVQMVDQQSQAAGGPAMSKFEVLTAMAFAAFAD
APVDVAVVEVGMGGRWDATNVVNAPVAVITPISIDHVDYLGHDLAGIAGEKAGIITRAPAGAPDTVAVIAQQPPEVMEVL
LAQCVQADAAVARAGSEFAVLGRQVAIGGQLLQLQGLGGVYSDVYLPLHGEHQAHNAALALAAVEAFFGAGAQRQLDVDA
VRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGAAALARTLADEFDFRFLVGVLSVLADKDVDGILAALQPVFHSVVVTH
NGSPRALDVESLALAAREQFGPDRVSTAENLSDAIDVATALVDDAVRDGLVDGQAEDFAGTGIVITGSVVTAGAARTLFG
RDPQ

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=387, Percent_Identity=29.1989664082687, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI66932984, Length=387, Percent_Identity=29.1989664082687, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI1788654, Length=376, Percent_Identity=31.6489361702128, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=454, Percent_Identity=24.6696035242291, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17553150, Length=454, Percent_Identity=24.6696035242291, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17553148, Length=454, Percent_Identity=24.8898678414097, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323760, Length=371, Percent_Identity=32.3450134770889, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6324815, Length=317, Percent_Identity=25.2365930599369, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24641571, Length=177, Percent_Identity=35.5932203389831, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24581568, Length=195, Percent_Identity=31.2820512820513, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 50785; Mature: 50654

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPPDWAPEHDVPDPVPTPDEIAALLQVEHLLDQRWPETKIEPSLTRISALLDLLGSPQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
RAYPSIHVAGTNGKTSVARMIDALVTALHRRTGRTTSPHLQSAVERISIDAKPISPARYV
HHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
DTYREIEPFVQMVDQQSQAAGGPAMSKFEVLTAMAFAAFADAPVDVAVVEVGMGGRWDAT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCC
NVVNAPVAVITPISIDHVDYLGHDLAGIAGEKAGIITRAPAGAPDTVAVIAQQPPEVMEV
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHH
LLAQCVQADAAVARAGSEFAVLGRQVAIGGQLLQLQGLGGVYSDVYLPLHGEHQAHNAAL
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHCEEEECCCCCCHHHHHH
ALAAVEAFFGAGAQRQLDVDAVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGAAALAR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCEEEEECCCCCHHHHHHHH
TLADEFDFRFLVGVLSVLADKDVDGILAALQPVFHSVVVTHNGSPRALDVESLALAAREQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEECCCCCEECHHHHHHHHHHH
FGPDRVSTAENLSDAIDVATALVDDAVRDGLVDGQAEDFAGTGIVITGSVVTAGAARTLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHCCCHHHCC
GRDPQ
CCCCC
>Mature Secondary Structure 
SEPPDWAPEHDVPDPVPTPDEIAALLQVEHLLDQRWPETKIEPSLTRISALLDLLGSPQ
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC
RAYPSIHVAGTNGKTSVARMIDALVTALHRRTGRTTSPHLQSAVERISIDAKPISPARYV
HHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
DTYREIEPFVQMVDQQSQAAGGPAMSKFEVLTAMAFAAFADAPVDVAVVEVGMGGRWDAT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCC
NVVNAPVAVITPISIDHVDYLGHDLAGIAGEKAGIITRAPAGAPDTVAVIAQQPPEVMEV
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHH
LLAQCVQADAAVARAGSEFAVLGRQVAIGGQLLQLQGLGGVYSDVYLPLHGEHQAHNAAL
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHCEEEECCCCCCHHHHHH
ALAAVEAFFGAGAQRQLDVDAVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGAAALAR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCEEEEECCCCCHHHHHHHH
TLADEFDFRFLVGVLSVLADKDVDGILAALQPVFHSVVVTHNGSPRALDVESLALAAREQ
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEECCCCCEECHHHHHHHHHHH
FGPDRVSTAENLSDAIDVATALVDDAVRDGLVDGQAEDFAGTGIVITGSVVTAGAARTLF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHCCCHHHCC
GRDPQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]