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The map label for this gene is mbtA

Identifier: 41408276

GI number: 41408276

Start: 2421552

End: 2422754

Strand: Direct

Name: mbtA

Synonym: MAP2178

Alternate gene names: 41408276

Gene position: 2421552-2422754 (Clockwise)

Preceding gene: 41408264

Following gene: 41408277

Centisome position: 50.14

GC content: 70.66

Gene sequence:

>1203_bases
ATGAGCCCCAACACCACGCCGCCGGCCGGAGTCCTCGACGGGTTCGTGCCGTTTCCCGCCGAGCGCGCCGCCGCCTACCG
GGCGGCCGGTTTGTGGACGGGGCGAGCCCTGGACACGATCCTGACCGACGCGGCCCGGCGGTGGCCCGACCGAACCGCCG
TCCTCGACGCCTCCGGCGGCACCGGTTTCAGCTACGCGGGCCTCGACGAGCAGGCTAACCGCGCCGCCGCAGGTTTGGCG
GACGCGGGTATCGCCCCCGGCGACCGGGTGCTGCTGCAGCTGCCGAACGGCTGCCAGTTCGCGGTGGCGCTGTTCGCGCT
GTTACGGGCGGGGGCGATCCCGGTGATGTGCCTGCCCGGCCACCGCGCCGCCGAGCTTGGGCATTTCGCCGCGCTCAGCC
AGGCCACCGCACTGCTGATCGCCGACACCGCAGCGGGTTTCGATTATCGAACGATGGCCGCGGGGCTCATCGAGGAGCAT
GAAGCGCTCGCCCACGTGATCGTCGACGGCGACCCGGGGCCGTTTCTGTCCTGGGCGCAGCTGTGCGAGCGTGCGCCCGC
GGGGCGGCCGGCGACGCCGGTCGATCCGGGATCGCCTGCGCTGCTTCTGGTTTCCGGTGGCACCACCGGCACACCAAAGC
TCATCCCGCGCACCCACAACGATTACGTCTTCAACGCCACCGCCAGCGCCGAACTGTGCGGGCTCACCCGCGACGACGTC
TATCTGGCGGTGCTGTCGGCCGGCCACAATTTCCCGCTCGCCTGCCCCGGCCTGCTCGGCGCGATGACGGTGGGCGCGAC
CACGGTGTTCGGCACCGACCCCAGCCCCGAGGCGGCCTTTGCCACCATCGCGCGCCACGGCGTCACGGTGACCGCCCTGG
TCCCGGCCCTGGCCAAACTATGGGCGCAGGCCTGCGAATGGGAGGACAATCCACCGAAGTCCTTGCGGCTCTTGCAAGTC
GGCGGCGCCAAGTTGGAGGCCGACGACGCCCGTGTGATCCGCAGCGCGTTGAGGGTGCGGCCGGCGAAGACGGCGTCCAG
CTCGGCCACGCAGGCCGCGCAGTTGATGCGCCGGGCCGAAACCCCCGAACCGCAAGGCACCTCCCGCAACTCGGTCACGG
CCATGCCGCAAGCATTCCAGAACCGCGCAGGGATGTCAGCAACTTTCCCGCACCTTCTATCGAAAAAAGCGTCGTGCAAG
TAG

Upstream 100 bases:

>100_bases
CCCTGAATTAGCACAGGCTGCCCTTAAATGATTTGGTTACCCTATCCTTACTCCCTGCGTCCCCGCTACTAGCCCCGTTC
GAAGCCCGCTTAGAGGAGCC

Downstream 100 bases:

>100_bases
AATCGACTTCTCGAACCGTAAGAAACGAAGATCTCCGAGGAACGTCATGGGTTCTGCGCGACGGCTCGCCCGGAAGACCT
CGACGCTCGCCGGCGCTCTG

Product: MbtA

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSPNTTPPAGVLDGFVPFPAERAAAYRAAGLWTGRALDTILTDAARRWPDRTAVLDASGGTGFSYAGLDEQANRAAAGLA
DAGIAPGDRVLLQLPNGCQFAVALFALLRAGAIPVMCLPGHRAAELGHFAALSQATALLIADTAAGFDYRTMAAGLIEEH
EALAHVIVDGDPGPFLSWAQLCERAPAGRPATPVDPGSPALLLVSGGTTGTPKLIPRTHNDYVFNATASAELCGLTRDDV
YLAVLSAGHNFPLACPGLLGAMTVGATTVFGTDPSPEAAFATIARHGVTVTALVPALAKLWAQACEWEDNPPKSLRLLQV
GGAKLEADDARVIRSALRVRPAKTASSSATQAAQLMRRAETPEPQGTSRNSVTAMPQAFQNRAGMSATFPHLLSKKASCK

Sequences:

>Translated_400_residues
MSPNTTPPAGVLDGFVPFPAERAAAYRAAGLWTGRALDTILTDAARRWPDRTAVLDASGGTGFSYAGLDEQANRAAAGLA
DAGIAPGDRVLLQLPNGCQFAVALFALLRAGAIPVMCLPGHRAAELGHFAALSQATALLIADTAAGFDYRTMAAGLIEEH
EALAHVIVDGDPGPFLSWAQLCERAPAGRPATPVDPGSPALLLVSGGTTGTPKLIPRTHNDYVFNATASAELCGLTRDDV
YLAVLSAGHNFPLACPGLLGAMTVGATTVFGTDPSPEAAFATIARHGVTVTALVPALAKLWAQACEWEDNPPKSLRLLQV
GGAKLEADDARVIRSALRVRPAKTASSSATQAAQLMRRAETPEPQGTSRNSVTAMPQAFQNRAGMSATFPHLLSKKASCK
>Mature_399_residues
SPNTTPPAGVLDGFVPFPAERAAAYRAAGLWTGRALDTILTDAARRWPDRTAVLDASGGTGFSYAGLDEQANRAAAGLAD
AGIAPGDRVLLQLPNGCQFAVALFALLRAGAIPVMCLPGHRAAELGHFAALSQATALLIADTAAGFDYRTMAAGLIEEHE
ALAHVIVDGDPGPFLSWAQLCERAPAGRPATPVDPGSPALLLVSGGTTGTPKLIPRTHNDYVFNATASAELCGLTRDDVY
LAVLSAGHNFPLACPGLLGAMTVGATTVFGTDPSPEAAFATIARHGVTVTALVPALAKLWAQACEWEDNPPKSLRLLQVG
GAKLEADDARVIRSALRVRPAKTASSSATQAAQLMRRAETPEPQGTSRNSVTAMPQAFQNRAGMSATFPHLLSKKASCK

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786810, Length=316, Percent_Identity=39.5569620253165, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI145693145, Length=283, Percent_Identity=30.0353356890459, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI221142682, Length=276, Percent_Identity=31.1594202898551, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24581924, Length=328, Percent_Identity=22.2560975609756, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.-

Molecular weight: Translated: 41459; Mature: 41327

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPNTTPPAGVLDGFVPFPAERAAAYRAAGLWTGRALDTILTDAARRWPDRTAVLDASGG
CCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
TGFSYAGLDEQANRAAAGLADAGIAPGDRVLLQLPNGCQFAVALFALLRAGAIPVMCLPG
CCCEECCCCHHHHHHHHCHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEECC
HRAAELGHFAALSQATALLIADTAAGFDYRTMAAGLIEEHEALAHVIVDGDPGPFLSWAQ
CHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHH
LCERAPAGRPATPVDPGSPALLLVSGGTTGTPKLIPRTHNDYVFNATASAELCGLTRDDV
HHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCE
YLAVLSAGHNFPLACPGLLGAMTVGATTVFGTDPSPEAAFATIARHGVTVTALVPALAKL
EEEEEECCCCCCCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
WAQACEWEDNPPKSLRLLQVGGAKLEADDARVIRSALRVRPAKTASSSATQAAQLMRRAE
HHHHHCCCCCCCHHEEEEEECCCEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
TPEPQGTSRNSVTAMPQAFQNRAGMSATFPHLLSKKASCK
CCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SPNTTPPAGVLDGFVPFPAERAAAYRAAGLWTGRALDTILTDAARRWPDRTAVLDASGG
CCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
TGFSYAGLDEQANRAAAGLADAGIAPGDRVLLQLPNGCQFAVALFALLRAGAIPVMCLPG
CCCEECCCCHHHHHHHHCHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEECC
HRAAELGHFAALSQATALLIADTAAGFDYRTMAAGLIEEHEALAHVIVDGDPGPFLSWAQ
CHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHH
LCERAPAGRPATPVDPGSPALLLVSGGTTGTPKLIPRTHNDYVFNATASAELCGLTRDDV
HHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCE
YLAVLSAGHNFPLACPGLLGAMTVGATTVFGTDPSPEAAFATIARHGVTVTALVPALAKL
EEEEEECCCCCCCCCHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHH
WAQACEWEDNPPKSLRLLQVGGAKLEADDARVIRSALRVRPAKTASSSATQAAQLMRRAE
HHHHHCCCCCCCHHEEEEEECCCEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
TPEPQGTSRNSVTAMPQAFQNRAGMSATFPHLLSKKASCK
CCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]