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The map label for this gene is gph [C]

Identifier: 41408082

GI number: 41408082

Start: 2190532

End: 2191200

Strand: Direct

Name: gph [C]

Synonym: MAP1984

Alternate gene names: 41408082

Gene position: 2190532-2191200 (Clockwise)

Preceding gene: 41408077

Following gene: 41408083

Centisome position: 45.35

GC content: 72.8

Gene sequence:

>669_bases
GTGACAGGCACAACGTCAGCCGATCCCGCCGCCCCCGCGGCGCAGCTGGTGATCTTCGATCTGGACGGCACGCTGACCGA
CTCCGCGGAGGGCATCGTGGCCAGCTTCCTGCATGCGCTCGAGTCCATCGGCGCGCCGGTGCCCCGGGGCGACCTCGTCG
CGCAGATCGTCGGCCCGCCGATGGACGACACGTTCCGGTCCATGCAACTCGGCGAGGACGCCGAGGCGGCGATCGCGGCG
TTCCGCGCCGAGTACGGCGCCCGCGGCTGGGCGATGAACACGCTGTTCGACGGGATCGAGGCGCTGCTGGCCGATCTGCG
CGCCGCCGGGATGCGGCTGGCGGTGGCCACCTCCAAGCTGGAGCCGACGGCGCGGCGCATCCTCGCCCATTTCGGGCTCG
ACCATCACTTCGAGGTGATCGCCGGGGCCAGCCCGGACCGTTCCCGCAAGGCCAAGGTCGAGGTGCTCGCGCACGCGCTG
GCGCAGCTGGAACCGCTGCCCGAGCGGGTGCTGATGGTCGGTGACCGCAGCCACGACGTGCACGGCGCGGCCGCGCACGG
CATCGACACCGTGGTGGTCGGCTGGGGCTACGGGCACGCCGACTCGCACCCCGACGGCGTCACCCGCGTCGCCACCATCG
ACGAGCTGCGGAGGGCGCTGGGTGTCTGA

Upstream 100 bases:

>100_bases
GACTCGCCATCCGTGAAACATTAGTGGCCGGCCCGGCGGCCCGGAGCAGCACCGAGCAACACCGTCCCGGCCCGCCGCGG
CCATCGAGGACAATGGTGAG

Downstream 100 bases:

>100_bases
ACCGCTGCACGTCACGTTCGTCTGCACCGGCAACATCTGCCGGTCGGTGATGGCCGAGAAGATGTTCGCCGACCAGCTGC
GCCGCCGCGGCTTGGCCGAT

Product: hypothetical protein

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MTGTTSADPAAPAAQLVIFDLDGTLTDSAEGIVASFLHALESIGAPVPRGDLVAQIVGPPMDDTFRSMQLGEDAEAAIAA
FRAEYGARGWAMNTLFDGIEALLADLRAAGMRLAVATSKLEPTARRILAHFGLDHHFEVIAGASPDRSRKAKVEVLAHAL
AQLEPLPERVLMVGDRSHDVHGAAAHGIDTVVVGWGYGHADSHPDGVTRVATIDELRRALGV

Sequences:

>Translated_222_residues
MTGTTSADPAAPAAQLVIFDLDGTLTDSAEGIVASFLHALESIGAPVPRGDLVAQIVGPPMDDTFRSMQLGEDAEAAIAA
FRAEYGARGWAMNTLFDGIEALLADLRAAGMRLAVATSKLEPTARRILAHFGLDHHFEVIAGASPDRSRKAKVEVLAHAL
AQLEPLPERVLMVGDRSHDVHGAAAHGIDTVVVGWGYGHADSHPDGVTRVATIDELRRALGV
>Mature_221_residues
TGTTSADPAAPAAQLVIFDLDGTLTDSAEGIVASFLHALESIGAPVPRGDLVAQIVGPPMDDTFRSMQLGEDAEAAIAAF
RAEYGARGWAMNTLFDGIEALLADLRAAGMRLAVATSKLEPTARRILAHFGLDHHFEVIAGASPDRSRKAKVEVLAHALA
QLEPLPERVLMVGDRSHDVHGAAAHGIDTVVVGWGYGHADSHPDGVTRVATIDELRRALGV

Specific function: Unknown

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=237, Percent_Identity=26.5822784810127, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 23338; Mature: 23206

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTTSADPAAPAAQLVIFDLDGTLTDSAEGIVASFLHALESIGAPVPRGDLVAQIVGPP
CCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
MDDTFRSMQLGEDAEAAIAAFRAEYGARGWAMNTLFDGIEALLADLRAAGMRLAVATSKL
CCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEHHCCC
EPTARRILAHFGLDHHFEVIAGASPDRSRKAKVEVLAHALAQLEPLPERVLMVGDRSHDV
CHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCC
HGAAAHGIDTVVVGWGYGHADSHPDGVTRVATIDELRRALGV
CCHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGTTSADPAAPAAQLVIFDLDGTLTDSAEGIVASFLHALESIGAPVPRGDLVAQIVGPP
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
MDDTFRSMQLGEDAEAAIAAFRAEYGARGWAMNTLFDGIEALLADLRAAGMRLAVATSKL
CCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEHHCCC
EPTARRILAHFGLDHHFEVIAGASPDRSRKAKVEVLAHALAQLEPLPERVLMVGDRSHDV
CHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCC
HGAAAHGIDTVVVGWGYGHADSHPDGVTRVATIDELRRALGV
CCHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12368430; 12218036 [H]