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The map label for this gene is glnA2

Identifier: 41408064

GI number: 41408064

Start: 2171350

End: 2172690

Strand: Reverse

Name: glnA2

Synonym: MAP1966c

Alternate gene names: 41408064

Gene position: 2172690-2171350 (Counterclockwise)

Preceding gene: 41408065

Following gene: 41408063

Centisome position: 44.99

GC content: 68.01

Gene sequence:

>1341_bases
ATGGATCGACAGAAGGAATTCGTCCTGCGCACCCTCGAGGAACGGGACATCCGCTTCGTTCGGCTGTGGTTCACCGATGT
GCTCGGGTTCCTCAAGTCGGTCGCCATCGCCCCGGCCGAACTCGAAGGCGCCTTCGAGGAGGGCATCGGCTTCGACGGCT
CCTCGATCGAGGGCTTCTCCCGGGTGTCGGAATCCGACACCGTCGCCAACCCCGACCCGTCCACCTTCCAGGTGTTGCCA
TGGGCCTCGCCCAACGGCCACCACCACTCGGCGCGAATGTTCTGCGACATCACCATGCCGGACGGGTCGCCGTCCTGGGC
CGACCCGCGGCACGTGCTGCGCCGCCAGCTGCAGAAGGCCAACGACCTGGGCTTCTCCTGCTACGTGCACCCCGAGATCG
AGTTCTTCCTGCTCAAGCCCGGGCCCGACGACGGCACGCCGCCCGTCCCCGTCGACACCGCCGGCTACTTCGACCAGGCC
ATCCACGACTCCGCCTCCAACTTCCGCAGGCACGCCATCGAAGCGCTGGAGTTCATGGGCATCTCGGTGGAGTTCAGCCA
CCACGAGGGCGCGCCCGGCCAGCAGGAGATCGACCTGCGCTTCGCCGACGCCCTGTCGATGGCCGACAACGTGATGACGT
TCCGCTACGTCATCAAGGAGGTCGCGATCGAGAACGGCGCCCGCGCCTCGTTCATGCCCAAGCCGTTCGGGCAGCATCCC
GGCTCGGCGATGCACACCCACATGAGCCTGTTCGAGGGCGACGTCAACGCGTTCCACAGCCCCGACGACCCGCTGCAACT
CTCGGACGTGGGCAAGTCGTTCATCGCCGGCATCCTCGAGCACGCCTCCGAGATCAGCGCCGTCACCAACCAATGGGTGA
ACTCCTACAAGCGGCTGGTGGGCGGCGGCGAGGCCCCCACCGCCGCGTCCTGGGGGGCGGCCAACCGCTCGGCGCTGGTG
CGGGTGCCGATGTACACGCCGCACAAGACGTCGTCGCGGCGCGTCGAGGTGCGCAGCCCCGACTCGGCGTGCAACCCCTA
CCTGACCTTCGCCGTGCTGCTGGCCGCCGGCCTGCGCGGGGTGGAGAAGGGCTACGTGCTGGGGCCGCAGGCCGAGGACA
ACGTGTGGGACCTGACCCCCGAGGAGCGGATCGCGATGGGCTATCGCGAACTGCCCACCAGCCTCGACAGCGCGCTGCGC
GCCATGGAGTCCTCGGAGCTGGTCGCGGAGACGTTGGGGGAGCACGTCTTTGACTTCTTCCTGCGCAACAAGCGCACCGA
GTGGGCGACCTACCGCAGCCACGTCACCCCGTACGAACTGCGCACCTACCTGTCGCTGTAG

Upstream 100 bases:

>100_bases
TATCGCTGCCAGTCCTGACCAGCGCCGCACGCCCCACCGCAACGGTTCTGAGCGGGCATCGGTAACACAGAATTAACAGC
GGTTGCCTACTGTTCGAGAT

Downstream 100 bases:

>100_bases
CCCCGGCGCCGTAGCGCATTTCCCGTCCCGGGTGCGCCGGCGCGGCGTCGGGTTGCGCTACCGTCGTGGTCGTGACCAAA
CCCGCGACGCAGCGCCCCCG

Product: GlnA2

Products: NA

Alternate protein names: Glutamate--ammonia ligase 2 [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIGFDGSSIEGFSRVSESDTVANPDPSTFQVLP
WASPNGHHHSARMFCDITMPDGSPSWADPRHVLRRQLQKANDLGFSCYVHPEIEFFLLKPGPDDGTPPVPVDTAGYFDQA
IHDSASNFRRHAIEALEFMGISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVIKEVAIENGARASFMPKPFGQHP
GSAMHTHMSLFEGDVNAFHSPDDPLQLSDVGKSFIAGILEHASEISAVTNQWVNSYKRLVGGGEAPTAASWGAANRSALV
RVPMYTPHKTSSRRVEVRSPDSACNPYLTFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERIAMGYRELPTSLDSALR
AMESSELVAETLGEHVFDFFLRNKRTEWATYRSHVTPYELRTYLSL

Sequences:

>Translated_446_residues
MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIGFDGSSIEGFSRVSESDTVANPDPSTFQVLP
WASPNGHHHSARMFCDITMPDGSPSWADPRHVLRRQLQKANDLGFSCYVHPEIEFFLLKPGPDDGTPPVPVDTAGYFDQA
IHDSASNFRRHAIEALEFMGISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVIKEVAIENGARASFMPKPFGQHP
GSAMHTHMSLFEGDVNAFHSPDDPLQLSDVGKSFIAGILEHASEISAVTNQWVNSYKRLVGGGEAPTAASWGAANRSALV
RVPMYTPHKTSSRRVEVRSPDSACNPYLTFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERIAMGYRELPTSLDSALR
AMESSELVAETLGEHVFDFFLRNKRTEWATYRSHVTPYELRTYLSL
>Mature_446_residues
MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIGFDGSSIEGFSRVSESDTVANPDPSTFQVLP
WASPNGHHHSARMFCDITMPDGSPSWADPRHVLRRQLQKANDLGFSCYVHPEIEFFLLKPGPDDGTPPVPVDTAGYFDQA
IHDSASNFRRHAIEALEFMGISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVIKEVAIENGARASFMPKPFGQHP
GSAMHTHMSLFEGDVNAFHSPDDPLQLSDVGKSFIAGILEHASEISAVTNQWVNSYKRLVGGGEAPTAASWGAANRSALV
RVPMYTPHKTSSRRVEVRSPDSACNPYLTFAVLLAAGLRGVEKGYVLGPQAEDNVWDLTPEERIAMGYRELPTSLDSALR
AMESSELVAETLGEHVFDFFLRNKRTEWATYRSHVTPYELRTYLSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=447, Percent_Identity=27.0693512304251, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1790301, Length=478, Percent_Identity=35.3556485355649, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87081870, Length=398, Percent_Identity=27.8894472361809, Blast_Score=124, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17540008, Length=220, Percent_Identity=28.6363636363636, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49595; Mature: 49595

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIGFDGSSIEGFS
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHH
RVSESDTVANPDPSTFQVLPWASPNGHHHSARMFCDITMPDGSPSWADPRHVLRRQLQKA
HHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
NDLGFSCYVHPEIEFFLLKPGPDDGTPPVPVDTAGYFDQAIHDSASNFRRHAIEALEFMG
CCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVIKEVAIENGARASFMPKPFGQHP
CEEEECCCCCCCCCHHEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GSAMHTHMSLFEGDVNAFHSPDDPLQLSDVGKSFIAGILEHASEISAVTNQWVNSYKRLV
CHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGGEAPTAASWGAANRSALVRVPMYTPHKTSSRRVEVRSPDSACNPYLTFAVLLAAGLRG
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
VEKGYVLGPQAEDNVWDLTPEERIAMGYRELPTSLDSALRAMESSELVAETLGEHVFDFF
CCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNKRTEWATYRSHVTPYELRTYLSL
HHCCCCHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MDRQKEFVLRTLEERDIRFVRLWFTDVLGFLKSVAIAPAELEGAFEEGIGFDGSSIEGFS
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHH
RVSESDTVANPDPSTFQVLPWASPNGHHHSARMFCDITMPDGSPSWADPRHVLRRQLQKA
HHCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHH
NDLGFSCYVHPEIEFFLLKPGPDDGTPPVPVDTAGYFDQAIHDSASNFRRHAIEALEFMG
CCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ISVEFSHHEGAPGQQEIDLRFADALSMADNVMTFRYVIKEVAIENGARASFMPKPFGQHP
CEEEECCCCCCCCCHHEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GSAMHTHMSLFEGDVNAFHSPDDPLQLSDVGKSFIAGILEHASEISAVTNQWVNSYKRLV
CHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGGEAPTAASWGAANRSALVRVPMYTPHKTSSRRVEVRSPDSACNPYLTFAVLLAAGLRG
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
VEKGYVLGPQAEDNVWDLTPEERIAMGYRELPTSLDSALRAMESSELVAETLGEHVFDFF
CCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNKRTEWATYRSHVTPYELRTYLSL
HHCCCCHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]