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The map label for this gene is polA [C]

Identifier: 41407923

GI number: 41407923

Start: 2004157

End: 2005113

Strand: Direct

Name: polA [C]

Synonym: MAP1825

Alternate gene names: 41407923

Gene position: 2004157-2005113 (Clockwise)

Preceding gene: 41407918

Following gene: 41407936

Centisome position: 41.5

GC content: 71.79

Gene sequence:

>957_bases
ATGCGATTGCCACTGGTGCTGCTCGACGGCGCCAGCATGTGGTTCCGCTCCTATTTCGGGGTGCCGTCCTCGATCACCGC
CCCCGACGGCCGGCCGGTAAACGCGGTACGCGGATTCCTCGATTCGCTCGCCGTGGTGATCACCCAGCAACGGCCCAGCC
GTCTCGTCGTCTGCCTGGACCTGGACTGGCGCCCGCAATTCCGGGTGGACCTGGTCCCGTCCTACAAGGCCCACCGGGTG
CCCGAGGCGGAGCCGGCGGGCGAGCCGGACGTCGAGGAGGTGCCGGACGATCTGACGCCCCAGATCGACATGATCGCCGA
ACTCCTTGAGGCGTACGGGATCCCGACGGCCGGGGCCGAGGGATTCGAGGCCGACGACGTGCTGGGCACCTTGGCGGCCC
GCGAGCGCGACGACCCGGTGGTGGTGGTCAGCGGCGATCGCGACCTGCTGCAGGTGGTGTCCGACGATCCCGTGCCGGTG
CGGGTGCTTTACCTCGGCCGCGGCCTGAGCAAAGCCACCCTGTTCGGCCCCGTCGAGGTGGCCGAACACTACGGCGTGCC
GGTCGACCGGGCCGGGCCGGCCTACGCCGAACTCGCGCTGCTGCGCGGTGACCCCTCCGACGGCCTGCCGGGTGTTCCGG
GCGTCGGCGAGAAGACCGCCGCGACCCTGCTGGCCCAGCACGGTTCGCTGGAGCGGGTGCTGGCCGCCGCCCACGACCCG
AAATCGAAGATGGCCAAGGGCCTGCGGGCCAAGCTGCTGGGGGCGCTGGACTACATCGAGGCGGCGGGCGCGGTGGTGCG
GGTGGCCACCGATGCGCCCGTCAAGCTGTCGACGCCCAGCGATGCAGTGCCGCTGGTCGCCGCCGATCCGCACCGCACCG
CCGAGCTGGCCACCGAGCTCGGCGTCGGCTCGTCGATCGCCCGACTGCAGAAAGCGCTCGACGCGCTGCCCGGCTAA

Upstream 100 bases:

>100_bases
TAGAGGCTGTTGCATCCAGCGCTAGAAGGTAAGCGAGAACGGGCCGCAACAACTCGACGCTCGACGGCCTGGCAACACGC
GGCGTGACAAGATAACCGGC

Downstream 100 bases:

>100_bases
CCGGTCACTGGGGGCGGCCCACCTCGTAGGTGCCCTTGTTGTCCTGGAAGGTCACCGTCACGTGCTTGGGCGCGCCGTCG
ATGCTGACGTCACAGTCGAA

Product: hypothetical protein

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MRLPLVLLDGASMWFRSYFGVPSSITAPDGRPVNAVRGFLDSLAVVITQQRPSRLVVCLDLDWRPQFRVDLVPSYKAHRV
PEAEPAGEPDVEEVPDDLTPQIDMIAELLEAYGIPTAGAEGFEADDVLGTLAARERDDPVVVVSGDRDLLQVVSDDPVPV
RVLYLGRGLSKATLFGPVEVAEHYGVPVDRAGPAYAELALLRGDPSDGLPGVPGVGEKTAATLLAQHGSLERVLAAAHDP
KSKMAKGLRAKLLGALDYIEAAGAVVRVATDAPVKLSTPSDAVPLVAADPHRTAELATELGVGSSIARLQKALDALPG

Sequences:

>Translated_318_residues
MRLPLVLLDGASMWFRSYFGVPSSITAPDGRPVNAVRGFLDSLAVVITQQRPSRLVVCLDLDWRPQFRVDLVPSYKAHRV
PEAEPAGEPDVEEVPDDLTPQIDMIAELLEAYGIPTAGAEGFEADDVLGTLAARERDDPVVVVSGDRDLLQVVSDDPVPV
RVLYLGRGLSKATLFGPVEVAEHYGVPVDRAGPAYAELALLRGDPSDGLPGVPGVGEKTAATLLAQHGSLERVLAAAHDP
KSKMAKGLRAKLLGALDYIEAAGAVVRVATDAPVKLSTPSDAVPLVAADPHRTAELATELGVGSSIARLQKALDALPG
>Mature_318_residues
MRLPLVLLDGASMWFRSYFGVPSSITAPDGRPVNAVRGFLDSLAVVITQQRPSRLVVCLDLDWRPQFRVDLVPSYKAHRV
PEAEPAGEPDVEEVPDDLTPQIDMIAELLEAYGIPTAGAEGFEADDVLGTLAARERDDPVVVVSGDRDLLQVVSDDPVPV
RVLYLGRGLSKATLFGPVEVAEHYGVPVDRAGPAYAELALLRGDPSDGLPGVPGVGEKTAATLLAQHGSLERVLAAAHDP
KSKMAKGLRAKLLGALDYIEAAGAVVRVATDAPVKLSTPSDAVPLVAADPHRTAELATELGVGSSIARLQKALDALPG

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=294, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918
- InterPro:   IPR003583 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 33531; Mature: 33531

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPLVLLDGASMWFRSYFGVPSSITAPDGRPVNAVRGFLDSLAVVITQQRPSRLVVCLD
CCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEE
LDWRPQFRVDLVPSYKAHRVPEAEPAGEPDVEEVPDDLTPQIDMIAELLEAYGIPTAGAE
CCCCCCEEEEECCCCHHCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC
GFEADDVLGTLAARERDDPVVVVSGDRDLLQVVSDDPVPVRVLYLGRGLSKATLFGPVEV
CCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHCCCCCCEEEEEECCCCCHHHCCCHHHH
AEHYGVPVDRAGPAYAELALLRGDPSDGLPGVPGVGEKTAATLLAQHGSLERVLAAAHDP
HHHCCCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCC
KSKMAKGLRAKLLGALDYIEAAGAVVRVATDAPVKLSTPSDAVPLVAADPHRTAELATEL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCEEECCCCHHHHHHHHH
GVGSSIARLQKALDALPG
CCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLPLVLLDGASMWFRSYFGVPSSITAPDGRPVNAVRGFLDSLAVVITQQRPSRLVVCLD
CCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEE
LDWRPQFRVDLVPSYKAHRVPEAEPAGEPDVEEVPDDLTPQIDMIAELLEAYGIPTAGAE
CCCCCCEEEEECCCCHHCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC
GFEADDVLGTLAARERDDPVVVVSGDRDLLQVVSDDPVPVRVLYLGRGLSKATLFGPVEV
CCCHHHHHHHHHHCCCCCCEEEEECCHHHHHHHCCCCCCEEEEEECCCCCHHHCCCHHHH
AEHYGVPVDRAGPAYAELALLRGDPSDGLPGVPGVGEKTAATLLAQHGSLERVLAAAHDP
HHHCCCCCCCCCCHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCC
KSKMAKGLRAKLLGALDYIEAAGAVVRVATDAPVKLSTPSDAVPLVAADPHRTAELATEL
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCCEEECCCCHHHHHHHHH
GVGSSIARLQKALDALPG
CCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]