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The map label for this gene is lysS

Identifier: 41406557

GI number: 41406557

Start: 485980

End: 487479

Strand: Direct

Name: lysS

Synonym: MAP0459

Alternate gene names: 41406557

Gene position: 485980-487479 (Clockwise)

Preceding gene: 41406556

Following gene: 41406558

Centisome position: 10.06

GC content: 66.8

Gene sequence:

>1500_bases
GTGAGTCCCGCCCCAGACGCCGACATTCCCGAGCAGTACCGGATCCGCCGGGACAAGCGCGCGCGGCTGCTCGCCGAGGG
GCACGACCCGTACCCGGTCGCCGTCGAACGCACCCACACGCTGGCGCAGGTGCGCGCCGCCCACCCCGACCTGCCCGTCG
ACACCGCGACCGACGACGTGGTCGGCGTCGCCGGCCGGGTGATCTTCGCGCGCAACTCCGGCAAGCTGTGCTTCGCCACG
CTGCAGGAGGGCGACGGCACCACCCTGCAGGTGATGGTCAGCCTGGACAAGGTGGGCCGCGAAGCCCTGGACGCGTGGAA
GGCCGACGTCGACCTGGGCGACATCGTCTCCGTGCACGGCACCGTGATCAGCTCCCGGCGCGGCGAACTGTCCGTGCTCG
CCGACTCGTGGCGGATGGCGTCCAAGTCGTTGCGTCCGCTGCCCGTCGCGCACAAGGAGATGAGCGAAGAGGCGCGGGTG
CGGCAGCGCTACGTCGACCTGATCGTGCGCCCGCAGGCGCGCACGGTCGCCCGGCAGCGGATCGCGGTGATCCGGGCGAT
CCGCAACGCCTTGGAACGGCGCGGGTTTCTCGAAGTCGAAACCCCAATGTTGCAGACGCTGGCCGGGGGCGCGGCCGCGC
GGCCGTTCGTCACCCATTCCAATGCGCTCGACATCGATCTCTACCTGCGGATCGCTCCGGAACTGTTCCTCAAGCGCTGC
GTCGTCGGCGGTTTCGATAAGGTTTTCGAACTAAATCGCGTGTTCCGAAACGAAGGTGCGGATTCCACCCATTCTCCGGA
ATTCTCCATGCTGGAGACCTACCAGGCCTACGGAACGTATGACGATTCGGCGGTGGTGACGCGCGAGCTTATTCAAGAGG
TGGCCGACGAGGCCATCGGAACCCGACAACTACCGCTGCCCGACGGCAGCGTCTACGACATAGACGGAGAATGGGCTTCT
ATAGAAATGTACCCGTCGCTGTCGGCGGCGCTCGGTGAGGAGATCACCCCGCAGACCACGGTGGAAAAGCTGCTCGGCAT
CGCCGAGCGGCTGGGCGTCGAGATCCCCCGCGACCGCGGCTACGGGCACGGCAAGATCGTCGAGGAGCTGTGGGAGCACA
CCGTGGGCGACACGCTCACCGCGCCCACGTTCGTGCGGGATTTCCCGGTCGAGACAACGCCTTTGACGCGTCAGCACCGC
AGCATCGACGGCGTCACCGAGAAATGGGACCTGTACGTGCGTGGGGTCGAGCTGGCCACCGGCTACTCGGAGTTGACCGA
TCCCGTGGTGCAGCGCGAGCGGTTCGCCGCCCAGGCGCGTGCGGCGGCCGCCGGCGACGACGAGGCCATGGTGCTCGACG
AAGACTTTCTCGCCGCGCTCGAGTATGGGATGCCGCCCTGCACCGGAACGGGAATGGGTATCGACCGGTTGTTGATGACA
TTGACCGGGCTGTCAATTCGGGAGACAGTTTTGTTCCCCATCGTTCGGCCGCATTCGTAG

Upstream 100 bases:

>100_bases
TTTTCGAACGCAACCGGGATGCGCAGCGCGGGCGGCTGAAAACGGCCCGGTGACGGCGTCGGGCGCGGCCTGGCGGCCGC
GTCATTTAAGCTGGCACGTC

Downstream 100 bases:

>100_bases
TTGAAAGTGCAACGCCGCAGAAAGTCCAACCCTGCGGCGTCCGTACAGATTGAGTATGCTGCGCTGTTATGGCAGATTGG
TGACCGGGGAGGTCGATCCA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase 1; LysRS 1 [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSPAPDADIPEQYRIRRDKRARLLAEGHDPYPVAVERTHTLAQVRAAHPDLPVDTATDDVVGVAGRVIFARNSGKLCFAT
LQEGDGTTLQVMVSLDKVGREALDAWKADVDLGDIVSVHGTVISSRRGELSVLADSWRMASKSLRPLPVAHKEMSEEARV
RQRYVDLIVRPQARTVARQRIAVIRAIRNALERRGFLEVETPMLQTLAGGAAARPFVTHSNALDIDLYLRIAPELFLKRC
VVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQAYGTYDDSAVVTRELIQEVADEAIGTRQLPLPDGSVYDIDGEWAS
IEMYPSLSAALGEEITPQTTVEKLLGIAERLGVEIPRDRGYGHGKIVEELWEHTVGDTLTAPTFVRDFPVETTPLTRQHR
SIDGVTEKWDLYVRGVELATGYSELTDPVVQRERFAAQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGIDRLLMT
LTGLSIRETVLFPIVRPHS

Sequences:

>Translated_499_residues
MSPAPDADIPEQYRIRRDKRARLLAEGHDPYPVAVERTHTLAQVRAAHPDLPVDTATDDVVGVAGRVIFARNSGKLCFAT
LQEGDGTTLQVMVSLDKVGREALDAWKADVDLGDIVSVHGTVISSRRGELSVLADSWRMASKSLRPLPVAHKEMSEEARV
RQRYVDLIVRPQARTVARQRIAVIRAIRNALERRGFLEVETPMLQTLAGGAAARPFVTHSNALDIDLYLRIAPELFLKRC
VVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQAYGTYDDSAVVTRELIQEVADEAIGTRQLPLPDGSVYDIDGEWAS
IEMYPSLSAALGEEITPQTTVEKLLGIAERLGVEIPRDRGYGHGKIVEELWEHTVGDTLTAPTFVRDFPVETTPLTRQHR
SIDGVTEKWDLYVRGVELATGYSELTDPVVQRERFAAQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGIDRLLMT
LTGLSIRETVLFPIVRPHS
>Mature_498_residues
SPAPDADIPEQYRIRRDKRARLLAEGHDPYPVAVERTHTLAQVRAAHPDLPVDTATDDVVGVAGRVIFARNSGKLCFATL
QEGDGTTLQVMVSLDKVGREALDAWKADVDLGDIVSVHGTVISSRRGELSVLADSWRMASKSLRPLPVAHKEMSEEARVR
QRYVDLIVRPQARTVARQRIAVIRAIRNALERRGFLEVETPMLQTLAGGAAARPFVTHSNALDIDLYLRIAPELFLKRCV
VGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQAYGTYDDSAVVTRELIQEVADEAIGTRQLPLPDGSVYDIDGEWASI
EMYPSLSAALGEEITPQTTVEKLLGIAERLGVEIPRDRGYGHGKIVEELWEHTVGDTLTAPTFVRDFPVETTPLTRQHRS
IDGVTEKWDLYVRGVELATGYSELTDPVVQRERFAAQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGIDRLLMTL
TGLSIRETVLFPIVRPHS

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=516, Percent_Identity=39.3410852713178, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI194272210, Length=516, Percent_Identity=39.3410852713178, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI45439306, Length=477, Percent_Identity=23.2704402515723, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1790571, Length=498, Percent_Identity=39.7590361445783, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1789256, Length=499, Percent_Identity=39.6793587174349, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI87082379, Length=334, Percent_Identity=32.0359281437126, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1788173, Length=277, Percent_Identity=27.7978339350181, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=514, Percent_Identity=37.7431906614786, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17535925, Length=515, Percent_Identity=37.4757281553398, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI71994340, Length=492, Percent_Identity=38.2113821138211, Blast_Score=317, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17551876, Length=490, Percent_Identity=25.1020408163265, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6320242, Length=517, Percent_Identity=38.1044487427466, Blast_Score=306, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6324256, Length=477, Percent_Identity=29.559748427673, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24640849, Length=488, Percent_Identity=39.344262295082, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24640851, Length=488, Percent_Identity=39.344262295082, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24584738, Length=270, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 55141; Mature: 55010

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPAPDADIPEQYRIRRDKRARLLAEGHDPYPVAVERTHTLAQVRAAHPDLPVDTATDDV
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCHH
VGVAGRVIFARNSGKLCFATLQEGDGTTLQVMVSLDKVGREALDAWKADVDLGDIVSVHG
HHCCCEEEEEECCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHH
TVISSRRGELSVLADSWRMASKSLRPLPVAHKEMSEEARVRQRYVDLIVRPQARTVARQR
HHHHCCCCCEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IAVIRAIRNALERRGFLEVETPMLQTLAGGAAARPFVTHSNALDIDLYLRIAPELFLKRC
HHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCCCEEECCCCEEEEEEEHHHHHHHHHHH
VVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQAYGTYDDSAVVTRELIQEVADEAIG
HHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
TRQLPLPDGSVYDIDGEWASIEMYPSLSAALGEEITPQTTVEKLLGIAERLGVEIPRDRG
CCCCCCCCCCEEECCCCEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
YGHGKIVEELWEHTVGDTLTAPTFVRDFPVETTPLTRQHRSIDGVTEKWDLYVRGVELAT
CCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCCEEEEEEEEEHHC
GYSELTDPVVQRERFAAQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGIDRLLMT
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHHHHH
LTGLSIRETVLFPIVRPHS
HCCCCHHHHHEECCCCCCC
>Mature Secondary Structure 
SPAPDADIPEQYRIRRDKRARLLAEGHDPYPVAVERTHTLAQVRAAHPDLPVDTATDDV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCHH
VGVAGRVIFARNSGKLCFATLQEGDGTTLQVMVSLDKVGREALDAWKADVDLGDIVSVHG
HHCCCEEEEEECCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHH
TVISSRRGELSVLADSWRMASKSLRPLPVAHKEMSEEARVRQRYVDLIVRPQARTVARQR
HHHHCCCCCEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IAVIRAIRNALERRGFLEVETPMLQTLAGGAAARPFVTHSNALDIDLYLRIAPELFLKRC
HHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCCCCEEECCCCEEEEEEEHHHHHHHHHHH
VVGGFDKVFELNRVFRNEGADSTHSPEFSMLETYQAYGTYDDSAVVTRELIQEVADEAIG
HHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
TRQLPLPDGSVYDIDGEWASIEMYPSLSAALGEEITPQTTVEKLLGIAERLGVEIPRDRG
CCCCCCCCCCEEECCCCEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
YGHGKIVEELWEHTVGDTLTAPTFVRDFPVETTPLTRQHRSIDGVTEKWDLYVRGVELAT
CCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCCEEEEEEEEEHHC
GYSELTDPVVQRERFAAQARAAAAGDDEAMVLDEDFLAALEYGMPPCTGTGMGIDRLLMT
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHHHHH
LTGLSIRETVLFPIVRPHS
HCCCCHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]