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The map label for this gene is rnt

Identifier: 52843158

GI number: 52843158

Start: 3354133

End: 3354756

Strand: Direct

Name: rnt

Synonym: lpg2964

Alternate gene names: 52843158

Gene position: 3354133-3354756 (Clockwise)

Preceding gene: 52843157

Following gene: 52843161

Centisome position: 98.72

GC content: 39.58

Gene sequence:

>624_bases
ATGGTTTCTCGCAATAATATTCTAAGTAACACCATTAAAAATCGCTTTCGTGGCTTTTTGCCTGTTGTTGTTGATGTAGA
AACTGCGGGAATAGACCCTCAAAAAAATGCTCTCCTGGAAATGTGTATTGTTTTATTGGATATGGATGAGCAAGGCTTGT
TAAAAAAGGATAGTAGTTATTTTGAACATATTTTACCTTTCCCGGGTGCAGAACTGGATCAAAAATCACTGGAATTCAAC
CAAATAGATCCTTACCAGCCTCTTCGTTTTGCAGTAGATGAACAAACTGCCCTTGAACGATTATTCAAACCCATCCATGA
CTTGCTAAAAAAAACCCGTTGCCAAAGAGCTGTTTTAGTAGGGCATAATGCCTGGTTTGATTTGTTATTCATCAAAGCGG
CAATCAAAAGAACCGGGATAAAATCACCTTTCCATGCCTTCACTTGCTTTGATACAGCAACATTAGCGGGTTTGATTTTT
GGAGAAACCGTATTGGCCAAAGCAGCTCAAGCAGCCGGATTACATTTTGACAGCCAGGAGGCGCATTCTGCTATTTATGA
TGCGGAAAAAACAGCTGATTTGTTTTGCCTTATGATAAACAAGTGGCGAATGCTGAACATCTGA

Upstream 100 bases:

>100_bases
GAACTCATTAAATCACCTCAAACTATCCCCAACTCAATGCCATTTGGATCAACTCATGTTGTGCCTATTGCTGCTGGAGA
CACTATAAACTGGAGTATTA

Downstream 100 bases:

>100_bases
TATCAGAAAAACATAAATAGCCTGGATTACCTAAAACAGTAACCCAGGAGTTTGATAGAAATTTTCTTGTGTTATGCTTC
ACAATAAAACACAACAACAA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MVSRNNILSNTIKNRFRGFLPVVVDVETAGIDPQKNALLEMCIVLLDMDEQGLLKKDSSYFEHILPFPGAELDQKSLEFN
QIDPYQPLRFAVDEQTALERLFKPIHDLLKKTRCQRAVLVGHNAWFDLLFIKAAIKRTGIKSPFHAFTCFDTATLAGLIF
GETVLAKAAQAAGLHFDSQEAHSAIYDAEKTADLFCLMINKWRMLNI

Sequences:

>Translated_207_residues
MVSRNNILSNTIKNRFRGFLPVVVDVETAGIDPQKNALLEMCIVLLDMDEQGLLKKDSSYFEHILPFPGAELDQKSLEFN
QIDPYQPLRFAVDEQTALERLFKPIHDLLKKTRCQRAVLVGHNAWFDLLFIKAAIKRTGIKSPFHAFTCFDTATLAGLIF
GETVLAKAAQAAGLHFDSQEAHSAIYDAEKTADLFCLMINKWRMLNI
>Mature_207_residues
MVSRNNILSNTIKNRFRGFLPVVVDVETAGIDPQKNALLEMCIVLLDMDEQGLLKKDSSYFEHILPFPGAELDQKSLEFN
QIDPYQPLRFAVDEQTALERLFKPIHDLLKKTRCQRAVLVGHNAWFDLLFIKAAIKRTGIKSPFHAFTCFDTATLAGLIF
GETVLAKAAQAAGLHFDSQEAHSAIYDAEKTADLFCLMINKWRMLNI

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=194, Percent_Identity=52.0618556701031, Blast_Score=207, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.- [C]

Molecular weight: Translated: 23399; Mature: 23399

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSRNNILSNTIKNRFRGFLPVVVDVETAGIDPQKNALLEMCIVLLDMDEQGLLKKDSSY
CCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHCCHHH
FEHILPFPGAELDQKSLEFNQIDPYQPLRFAVDEQTALERLFKPIHDLLKKTRCQRAVLV
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEE
GHNAWFDLLFIKAAIKRTGIKSPFHAFTCFDTATLAGLIFGETVLAKAAQAAGLHFDSQE
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AHSAIYDAEKTADLFCLMINKWRMLNI
HHHHHHCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVSRNNILSNTIKNRFRGFLPVVVDVETAGIDPQKNALLEMCIVLLDMDEQGLLKKDSSY
CCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHCCHHH
FEHILPFPGAELDQKSLEFNQIDPYQPLRFAVDEQTALERLFKPIHDLLKKTRCQRAVLV
HHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEE
GHNAWFDLLFIKAAIKRTGIKSPFHAFTCFDTATLAGLIFGETVLAKAAQAAGLHFDSQE
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AHSAIYDAEKTADLFCLMINKWRMLNI
HHHHHHCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA