Click here to switch to the map view.

The map label for this gene is pyrC

Identifier: 52843157

GI number: 52843157

Start: 3353070

End: 3354149

Strand: Direct

Name: pyrC

Synonym: lpg2963

Alternate gene names: 52843157

Gene position: 3353070-3354149 (Clockwise)

Preceding gene: 52843149

Following gene: 52843158

Centisome position: 98.68

GC content: 40.19

Gene sequence:

>1080_bases
ATGTTATTCTCTTTTATCGGCAATAATGCAGGAACCTTTATTATGCAAACTCTGATAATTAATCGCCCTGACGATTGGCA
TCTCCATCTCAGGGATGGAGAGTTTCTAACTCATACCGTCCAGGCGTCTGCCCAACATTTTGCCAGAGCGTTAGTGATGC
CCAATCTCAAACCAGCACTAACCAATTTATCTGCAATTGAGAGTTATAGAAACAGAATCATTTCGGCCATTCCCAAACAA
AACTCCTTTATTCCTTATATGACTTTTTATTTAAATGAGTCCGTCAAACCAGAAGAATTACAGCAAGCCGCCTCTCTTTC
TTATATAGTTGGAGCAAAACTTTATCCAGCTGGAGCGACGACAAATTCTGAAGAGGGTGCTAAATCTTTGAAAGCACTCT
ACCCCCTTTTTGAAACATTGCAAGCGAATAATTTAGTCTTGCAAATTCATGGCGAAGTCACTCATAGCGATATTTTCGAG
CGTGAAGCATTATTTATTGATGAATACCTGAAACCTATCGCCAAACATTTCCCCAAATTAAGAATCGTGCTGGAGCACAT
ATCGACTAAAACTGCCGTGGATTATGTTTCTGAAGGGCCTGCTAACCTTACAGCAACAATAACTCCTCACCATTTGCTTT
ATAATCGCAATAAGCTACTCGCAGGGGGCATTAGACCTCATTATTATTGTTTGCCTATTTTAAAACACGAAAAAGACCAA
AAGGCCTTACAAATTGCAGCGACAAGCGGAAATCCAAAATTTTTTGCCGGCACTGACAGTGCCCCTCATGCCATCAATGC
CAAGGAAAATGCATGTGGATGCGCCGGTATTTATTCAGCTCCATATGCGGTAGCGCTCTACACGCAGATCTTTGATGAAC
TTAACCAATTAAAAAAACTTAATGATTTTCTTAGTCGATTTGGTGCAGAGTTTTACCAACTCCCTATTAATCAACAGCAA
TTGGAACTCATTAAATCACCTCAAACTATCCCCAACTCAATGCCATTTGGATCAACTCATGTTGTGCCTATTGCTGCTGG
AGACACTATAAACTGGAGTATTAATGGTTTCTCGCAATAA

Upstream 100 bases:

>100_bases
CGACAGTAGTAATGAATAAAGAACTCATTGCAATAATTTTTGCCTTCTTGCATATAAAATGCTCTGAAATCATTTTTTGC
TCATCCAAATTGCCTAATTC

Downstream 100 bases:

>100_bases
TATTCTAAGTAACACCATTAAAAATCGCTTTCGTGGCTTTTTGCCTGTTGTTGTTGATGTAGAAACTGCGGGAATAGACC
CTCAAAAAAATGCTCTCCTG

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MLFSFIGNNAGTFIMQTLIINRPDDWHLHLRDGEFLTHTVQASAQHFARALVMPNLKPALTNLSAIESYRNRIISAIPKQ
NSFIPYMTFYLNESVKPEELQQAASLSYIVGAKLYPAGATTNSEEGAKSLKALYPLFETLQANNLVLQIHGEVTHSDIFE
REALFIDEYLKPIAKHFPKLRIVLEHISTKTAVDYVSEGPANLTATITPHHLLYNRNKLLAGGIRPHYYCLPILKHEKDQ
KALQIAATSGNPKFFAGTDSAPHAINAKENACGCAGIYSAPYAVALYTQIFDELNQLKKLNDFLSRFGAEFYQLPINQQQ
LELIKSPQTIPNSMPFGSTHVVPIAAGDTINWSINGFSQ

Sequences:

>Translated_359_residues
MLFSFIGNNAGTFIMQTLIINRPDDWHLHLRDGEFLTHTVQASAQHFARALVMPNLKPALTNLSAIESYRNRIISAIPKQ
NSFIPYMTFYLNESVKPEELQQAASLSYIVGAKLYPAGATTNSEEGAKSLKALYPLFETLQANNLVLQIHGEVTHSDIFE
REALFIDEYLKPIAKHFPKLRIVLEHISTKTAVDYVSEGPANLTATITPHHLLYNRNKLLAGGIRPHYYCLPILKHEKDQ
KALQIAATSGNPKFFAGTDSAPHAINAKENACGCAGIYSAPYAVALYTQIFDELNQLKKLNDFLSRFGAEFYQLPINQQQ
LELIKSPQTIPNSMPFGSTHVVPIAAGDTINWSINGFSQ
>Mature_359_residues
MLFSFIGNNAGTFIMQTLIINRPDDWHLHLRDGEFLTHTVQASAQHFARALVMPNLKPALTNLSAIESYRNRIISAIPKQ
NSFIPYMTFYLNESVKPEELQQAASLSYIVGAKLYPAGATTNSEEGAKSLKALYPLFETLQANNLVLQIHGEVTHSDIFE
REALFIDEYLKPIAKHFPKLRIVLEHISTKTAVDYVSEGPANLTATITPHHLLYNRNKLLAGGIRPHYYCLPILKHEKDQ
KALQIAATSGNPKFFAGTDSAPHAINAKENACGCAGIYSAPYAVALYTQIFDELNQLKKLNDFLSRFGAEFYQLPINQQQ
LELIKSPQTIPNSMPFGSTHVVPIAAGDTINWSINGFSQ

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787301, Length=341, Percent_Identity=46.6275659824047, Blast_Score=344, Evalue=4e-96,
Organism=Saccharomyces cerevisiae, GI6323452, Length=368, Percent_Identity=34.7826086956522, Blast_Score=199, Evalue=5e-52,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 39971; Mature: 39971

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSFIGNNAGTFIMQTLIINRPDDWHLHLRDGEFLTHTVQASAQHFARALVMPNLKPAL
CEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHH
TNLSAIESYRNRIISAIPKQNSFIPYMTFYLNESVKPEELQQAASLSYIVGAKLYPAGAT
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCEEECCCCC
TNSEEGAKSLKALYPLFETLQANNLVLQIHGEVTHSDIFEREALFIDEYLKPIAKHFPKL
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHH
RIVLEHISTKTAVDYVSEGPANLTATITPHHLLYNRNKLLAGGIRPHYYCLPILKHEKDQ
HHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHEECCCCEEECCCCCCEEEEEHHHCCCCC
KALQIAATSGNPKFFAGTDSAPHAINAKENACGCAGIYSAPYAVALYTQIFDELNQLKKL
CEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NDFLSRFGAEFYQLPINQQQLELIKSPQTIPNSMPFGSTHVVPIAAGDTINWSINGFSQ
HHHHHHHCCHHEECCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCC
>Mature Secondary Structure
MLFSFIGNNAGTFIMQTLIINRPDDWHLHLRDGEFLTHTVQASAQHFARALVMPNLKPAL
CEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHH
TNLSAIESYRNRIISAIPKQNSFIPYMTFYLNESVKPEELQQAASLSYIVGAKLYPAGAT
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCEEECCCCC
TNSEEGAKSLKALYPLFETLQANNLVLQIHGEVTHSDIFEREALFIDEYLKPIAKHFPKL
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHH
RIVLEHISTKTAVDYVSEGPANLTATITPHHLLYNRNKLLAGGIRPHYYCLPILKHEKDQ
HHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHEECCCCEEECCCCCCEEEEEHHHCCCCC
KALQIAATSGNPKFFAGTDSAPHAINAKENACGCAGIYSAPYAVALYTQIFDELNQLKKL
CEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NDFLSRFGAEFYQLPINQQQLELIKSPQTIPNSMPFGSTHVVPIAAGDTINWSINGFSQ
HHHHHHHCCHHEECCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA