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The map label for this gene is rsmE [H]

Identifier: 52843130

GI number: 52843130

Start: 3321700

End: 3322434

Strand: Reverse

Name: rsmE [H]

Synonym: lpg2936

Alternate gene names: 52843130

Gene position: 3322434-3321700 (Counterclockwise)

Preceding gene: 52843132

Following gene: 52843129

Centisome position: 97.78

GC content: 45.71

Gene sequence:

>735_bases
TTGGCTGTGAGAACAATACGTATCTATCAACCTGGCGAATACCAGCCGGGGCAACTTCTGGAATTATCGCCTGAGGCGGG
GCAGCATGTTGGCGTAGTCTTGCGCATGGAGCAAGGTGAACAACTGACACTGTTTAATGGGGACAACAAAGAGTTTACAG
CATCTATTGAGAGGGTCAAAAAAAAGCAGGTATTCGTTAGAATTGCCTCTGTTCTTGAGGTGAACAGGGAATCCCCATTA
AAGATACATTTGGCTCAAGCCATTTCCAAGGGCGAGCGGATGGAAATGGTGATGCAAAAATCGGCAGAGTTAGGAGTGGC
ATGCATTACACCTCTCATTACCGAGCGTTGCCAGGTTAAAATAGACAAAGAAAAAATGGCAAAAAAAATGCACCAATGGC
TTAATATTATCATTGGAGCTTGCGAGCAATGTGGGCGTAATCAAATTCCAGAGTTGAGACAGCCAGTTTATTTGGATCAA
TTTGTCAGAGAAGCCAAAGAACATCTCAAGCTCATTTTGCATCCGGCATTTAGCAAGACATGGCGCGATTATCCTGTCCA
ACCTCCTGATGTTGCCTTGATTATTGGCCCGGAAGGTGGTTTCAGTGATGAGGAAATTAGATTGACCAGTGGTCATGGAT
TTCTGCCTTTGTCACTGGGGCCGCGAGTATTGCGTACGGAAACCGCGGCAATAACTGCGTTAAGTGTTTTGCAAGCTGCC
GGAGGTGATCTATAA

Upstream 100 bases:

>100_bases
AGCGAGTTGGGAAAGAATAAAATCAATCGCCAAAATTCTAGCATAGCAGACGTAAATTGGTAATTTTTGTTATAGTATCC
TTTACTAAAACCAAGGATAA

Downstream 100 bases:

>100_bases
TAGACTTCTTTCCAAATCGGTATAAGCTGCGTCTTTTGTTTTATAGCTCTATTACAAGTATTTCTGGAGTATTATATGTA
AACTACTCTTTTTGAAAATC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MAVRTIRIYQPGEYQPGQLLELSPEAGQHVGVVLRMEQGEQLTLFNGDNKEFTASIERVKKKQVFVRIASVLEVNRESPL
KIHLAQAISKGERMEMVMQKSAELGVACITPLITERCQVKIDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQ
FVREAKEHLKLILHPAFSKTWRDYPVQPPDVALIIGPEGGFSDEEIRLTSGHGFLPLSLGPRVLRTETAAITALSVLQAA
GGDL

Sequences:

>Translated_244_residues
MAVRTIRIYQPGEYQPGQLLELSPEAGQHVGVVLRMEQGEQLTLFNGDNKEFTASIERVKKKQVFVRIASVLEVNRESPL
KIHLAQAISKGERMEMVMQKSAELGVACITPLITERCQVKIDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQ
FVREAKEHLKLILHPAFSKTWRDYPVQPPDVALIIGPEGGFSDEEIRLTSGHGFLPLSLGPRVLRTETAAITALSVLQAA
GGDL
>Mature_243_residues
AVRTIRIYQPGEYQPGQLLELSPEAGQHVGVVLRMEQGEQLTLFNGDNKEFTASIERVKKKQVFVRIASVLEVNRESPLK
IHLAQAISKGERMEMVMQKSAELGVACITPLITERCQVKIDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQF
VREAKEHLKLILHPAFSKTWRDYPVQPPDVALIIGPEGGFSDEEIRLTSGHGFLPLSLGPRVLRTETAAITALSVLQAAG
GDL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=242, Percent_Identity=48.3471074380165, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27286; Mature: 27155

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRTIRIYQPGEYQPGQLLELSPEAGQHVGVVLRMEQGEQLTLFNGDNKEFTASIERVK
CCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHH
KKQVFVRIASVLEVNRESPLKIHLAQAISKGERMEMVMQKSAELGVACITPLITERCQVK
HHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHEE
IDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQFVREAKEHLKLILHPAFSKT
ECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHEEHHHHHHHH
WRDYPVQPPDVALIIGPEGGFSDEEIRLTSGHGFLPLSLGPRVLRTETAAITALSVLQAA
CCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHC
GGDL
CCCC
>Mature Secondary Structure 
AVRTIRIYQPGEYQPGQLLELSPEAGQHVGVVLRMEQGEQLTLFNGDNKEFTASIERVK
CEEEEEEECCCCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHH
KKQVFVRIASVLEVNRESPLKIHLAQAISKGERMEMVMQKSAELGVACITPLITERCQVK
HHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHEE
IDKEKMAKKMHQWLNIIIGACEQCGRNQIPELRQPVYLDQFVREAKEHLKLILHPAFSKT
ECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHEEHHHHHHHH
WRDYPVQPPDVALIIGPEGGFSDEEIRLTSGHGFLPLSLGPRVLRTETAAITALSVLQAA
CCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHC
GGDL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]