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The map label for this gene is rho

Identifier: 52843128

GI number: 52843128

Start: 3319576

End: 3320847

Strand: Reverse

Name: rho

Synonym: lpg2934

Alternate gene names: 52843128

Gene position: 3320847-3319576 (Counterclockwise)

Preceding gene: 52843129

Following gene: 52843127

Centisome position: 97.74

GC content: 44.65

Gene sequence:

>1272_bases
GTGAGAAGTATGAATCTTAGTGAACTTAAGCAATTACCTATTGCCGATCTCGTTAATATCGCACAAGAGATGGGTGTGGA
AAATACCTCCCGTATGCGCAAGCAAGACATCATTTTCGCCATCCTTAAAGCCCATGCTTTAAAAGGGGAAGACATCCATG
GCGATGGTGTTTTGGAAGTTTTGACTGATGGTTTTGGTTTCTTGCGTTCCGCTGATGGCTCCTATCTGGCAGGCCCTGAT
GACATTTACGTCTCTCCCAGTCAAATCAGACGTTTCGGTCTTCGTTCGGGGGATACTATTTCTGGTAAAATCCGCCCCCC
CAAAGACAGCGAACGCTATTTTGCTTTATTAAAAGTTGATCAAATTAACTACGATACACCGGATAGCGCAAAGCGTAAGA
TTTTATTTGAAAACTTAACTCCCTTGTTTGCTACCGAGCGTCTGGTTATGGAGCAAGGTAATGGCAGCACAGAAGATCTC
ACGGCAAGAGTAGTTGATTTATGCGCTCCTTTCGGCCGCGGACAGCGTGGTTTGATCGTGTCACCTCCGAAAGCAGGTAA
GACATTAATGTTGCAAAACATTGCTCGCTCTATAGAAAAAAATTATCCTGAATGTTATCTCATTGTTTTGCTGATTGATG
AACGTCCTGAAGAAGTAACGGAAATGCAGCGTTCTGTCAAAGGTGAAGTTGTTGCGAGTACTTTTGACGAGCCCGCCAAT
CGCCATGTTCAGGTTGCCGAAATGGTAATAGAAAAAGCAAAACGTCTGGTTGAGCATAAACGTGATGTGGTTATTCTGCT
GGATTCGATCACTCGCTTGGCCAGAGCTTACAATACGGTTGTACCGGCATCAGGTAAAGTACTGACTGGTGGTGTTGATG
CGAATGCCCTGCAAAGGCCCAAAAGATTGTATGGTGCGGCACGTAACATAGAAGAGGGCGGCAGTTTAACCATTATTGCA
ACGGCTCTGGTAGACACGGGTTCCAAAATGGACGAAGTGATTTACGAAGAGTTCAAAGGTACAGGTAACATGGAAATCCA
CTTGAGTCGTAATATTGCCGAGAGAAGGGTTTTCCCTGCGATTAACATTAATCGCTCTGGTACTCGCCGTGAAGATCTCC
TGTTGAGCCCCGAAGAATTACAGCGCACCTGGATATTGCGTAAAATCCTGCAATCCATGGATGAATGCGATGCGATTGAA
TTCTTATTGGAACGCATGAAGAATCATAAGACAAATGCCGAATTCTTTGATGCAATGAAGCGTCAGGAATAA

Upstream 100 bases:

>100_bases
CTTTAAAACAAGTGAGCCACATAGGCAGCGGATTCCTTAGCTGCTATTCCCTAACATAATTTTTTCTTCGGATATTCAAT
TCATTTAAACAACAAATCAA

Downstream 100 bases:

>100_bases
TTTAAATGATAATTAACCCCGTAATTAGCGGAGCATAATTCGGGGTTCAAATTCGCCTTATTTTCAGGTTTATAATTCAT
GCTCATAGCCTGTATTACGC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MRSMNLSELKQLPIADLVNIAQEMGVENTSRMRKQDIIFAILKAHALKGEDIHGDGVLEVLTDGFGFLRSADGSYLAGPD
DIYVSPSQIRRFGLRSGDTISGKIRPPKDSERYFALLKVDQINYDTPDSAKRKILFENLTPLFATERLVMEQGNGSTEDL
TARVVDLCAPFGRGQRGLIVSPPKAGKTLMLQNIARSIEKNYPECYLIVLLIDERPEEVTEMQRSVKGEVVASTFDEPAN
RHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRLYGAARNIEEGGSLTIIA
TALVDTGSKMDEVIYEEFKGTGNMEIHLSRNIAERRVFPAININRSGTRREDLLLSPEELQRTWILRKILQSMDECDAIE
FLLERMKNHKTNAEFFDAMKRQE

Sequences:

>Translated_423_residues
MRSMNLSELKQLPIADLVNIAQEMGVENTSRMRKQDIIFAILKAHALKGEDIHGDGVLEVLTDGFGFLRSADGSYLAGPD
DIYVSPSQIRRFGLRSGDTISGKIRPPKDSERYFALLKVDQINYDTPDSAKRKILFENLTPLFATERLVMEQGNGSTEDL
TARVVDLCAPFGRGQRGLIVSPPKAGKTLMLQNIARSIEKNYPECYLIVLLIDERPEEVTEMQRSVKGEVVASTFDEPAN
RHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRLYGAARNIEEGGSLTIIA
TALVDTGSKMDEVIYEEFKGTGNMEIHLSRNIAERRVFPAININRSGTRREDLLLSPEELQRTWILRKILQSMDECDAIE
FLLERMKNHKTNAEFFDAMKRQE
>Mature_423_residues
MRSMNLSELKQLPIADLVNIAQEMGVENTSRMRKQDIIFAILKAHALKGEDIHGDGVLEVLTDGFGFLRSADGSYLAGPD
DIYVSPSQIRRFGLRSGDTISGKIRPPKDSERYFALLKVDQINYDTPDSAKRKILFENLTPLFATERLVMEQGNGSTEDL
TARVVDLCAPFGRGQRGLIVSPPKAGKTLMLQNIARSIEKNYPECYLIVLLIDERPEEVTEMQRSVKGEVVASTFDEPAN
RHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRLYGAARNIEEGGSLTIIA
TALVDTGSKMDEVIYEEFKGTGNMEIHLSRNIAERRVFPAININRSGTRREDLLLSPEELQRTWILRKILQSMDECDAIE
FLLERMKNHKTNAEFFDAMKRQE

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=77.9904306220096, Blast_Score=689, Evalue=0.0,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47488; Mature: 47488

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSMNLSELKQLPIADLVNIAQEMGVENTSRMRKQDIIFAILKAHALKGEDIHGDGVLEV
CCCCCHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LTDGFGFLRSADGSYLAGPDDIYVSPSQIRRFGLRSGDTISGKIRPPKDSERYFALLKVD
HHCCHHHHCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEE
QINYDTPDSAKRKILFENLTPLFATERLVMEQGNGSTEDLTARVVDLCAPFGRGQRGLIV
ECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEE
SPPKAGKTLMLQNIARSIEKNYPECYLIVLLIDERPEEVTEMQRSVKGEVVASTFDEPAN
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCC
RHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRP
CHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHH
KRLYGAARNIEEGGSLTIIATALVDTGSKMDEVIYEEFKGTGNMEIHLSRNIAERRVFPA
HHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCC
ININRSGTRREDLLLSPEELQRTWILRKILQSMDECDAIEFLLERMKNHKTNAEFFDAMK
EECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RQE
CCC
>Mature Secondary Structure
MRSMNLSELKQLPIADLVNIAQEMGVENTSRMRKQDIIFAILKAHALKGEDIHGDGVLEV
CCCCCHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LTDGFGFLRSADGSYLAGPDDIYVSPSQIRRFGLRSGDTISGKIRPPKDSERYFALLKVD
HHCCHHHHCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEE
QINYDTPDSAKRKILFENLTPLFATERLVMEQGNGSTEDLTARVVDLCAPFGRGQRGLIV
ECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEE
SPPKAGKTLMLQNIARSIEKNYPECYLIVLLIDERPEEVTEMQRSVKGEVVASTFDEPAN
CCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCC
RHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRP
CHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHH
KRLYGAARNIEEGGSLTIIATALVDTGSKMDEVIYEEFKGTGNMEIHLSRNIAERRVFPA
HHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCC
ININRSGTRREDLLLSPEELQRTWILRKILQSMDECDAIEFLLERMKNHKTNAEFFDAMK
EECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RQE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]