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The map label for this gene is amiC

Identifier: 52843048

GI number: 52843048

Start: 3227502

End: 3228890

Strand: Reverse

Name: amiC

Synonym: lpg2852

Alternate gene names: 52843048

Gene position: 3228890-3227502 (Counterclockwise)

Preceding gene: 52843049

Following gene: 52843047

Centisome position: 95.03

GC content: 41.11

Gene sequence:

>1389_bases
TTGAGTATGCATGTTAATGAGTATATCCACTATGATGTGCAGGAGCTTGCTGAACTGATCAAAAACCATCAGGTTGGTGC
TGAGGAAGTTTTGCGGTGTGCGCAAGAACGCCTATATCAAGTGAATCCTTCATTAAATGCCGTAGTTACAGATTGTTTTG
AATATGCGCATCAATGCCTTGCTAAAATGAAAGGTAACGAGCCCTATTATGGTGTTCCGTTATTAGTCAAGGATCTGGGC
CATGCCATAGAGGGCATTCGACTAACGGAAGGGTCAAGGTTTTTTTCAAATAATCTGGCTGAGACAAACAGTGATTTGAT
TAATAAATTGATTGCTCTTGGTTTTGTACCATTTGCAAAAACAAATACTTCAGAATTAGGCCTTTCCTGCGTTACTGACT
CTAATTTATTTGGGCCTTGCAGAAATCCTTTTGATACTATGAGAACGGCAGGCGGATCTTCTGGTGGTTCTGCTGCTGCC
ATAATGGCAGGGATAGCGCCTGTGGCTACGGCAAGCGATGGAGGCGGGTCGATTCGTATTCCAGCGGCTTGTTGTGGTTT
GTTTGGATTCAAACCGACACCAGGTCTGGTTCCCTCAGGGCCATTGGTTGATGAAGCATGGTCCGGGATGGCTACCAGTT
TTATACTAACTCGTTCCATGAGTGATGCTATTGCATTGTTTAAAAATGTAATAAACCCCATGCGACTTAACAGACTATCG
AGAAAGAAAAAAAAATTACTTATTACCGAGTTGACAGGCGCTTTTGCAGAGGTACCAATTGCAAGGGAATGCCAGGAGGC
GGTAGAGAAAATAAAAGAAATCCTGAAAAATTCAGGTCACAGATTAAATGAAGTGCACTTGCCATTGGATCTGCAAGTTA
TCAGTTCATGCGCCCATACTTTAATTGCAGCGAGCACGTATGCGAAAATAAAAACTCAAGAACAAATCAGTGGCAGAAAA
CCAATGATTCATGAATTGGAACCCATAACATGGGAATTTTATTACAGAGGAAAAATGGTCTCCGCTTATGAATTCATTAT
TGCCAAAAACAAACTATATCAATCACTAAGACCTCTGCATCAATTATTGGAAAAAACAGACATTGTATTAACTCCTGCAT
TGGCTCAATTACCCCTGTTAATCGGTCATTTGCGCACTGATGAGGAATTCAATAAGTATCTACAAAAAAAGAAAGAGTTT
TCACCTTTTACTTCCCTGTTTAATCAAGGTGGCTTACCCGCTATGACTGTACCAGTTATGTTCCACCATCATTTGCCTGT
CTCTGTGCAAATGGGTTCAGCAAAAGGCAATGATTTGCTCTTATTGGAGTTGGCTCATGAATTACAGTCTGTGCTGCCAA
AAAATATCACGCCTGCATCAATTCCTTGA

Upstream 100 bases:

>100_bases
GCTGGCTCAGGCGACTATGATAACAACAGCTATAATGTTATAGATAACCTTGTAAAGAGTAATGTTCTCTCTTTATAAAG
CTCTCTGGCTATTGTAGGAA

Downstream 100 bases:

>100_bases
TGCTGATTTTTTGTAAGTTTTCATTTATCGAACCTTGTGTTAAGTTAACTGTAGCTTTTCATAAAATTAATAATAAGTGA
TTGTTTTTTTATAGCTATAG

Product: amidase, 6-aminohexanoate-cyclic-dimer hydrolase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSMHVNEYIHYDVQELAELIKNHQVGAEEVLRCAQERLYQVNPSLNAVVTDCFEYAHQCLAKMKGNEPYYGVPLLVKDLG
HAIEGIRLTEGSRFFSNNLAETNSDLINKLIALGFVPFAKTNTSELGLSCVTDSNLFGPCRNPFDTMRTAGGSSGGSAAA
IMAGIAPVATASDGGGSIRIPAACCGLFGFKPTPGLVPSGPLVDEAWSGMATSFILTRSMSDAIALFKNVINPMRLNRLS
RKKKKLLITELTGAFAEVPIARECQEAVEKIKEILKNSGHRLNEVHLPLDLQVISSCAHTLIAASTYAKIKTQEQISGRK
PMIHELEPITWEFYYRGKMVSAYEFIIAKNKLYQSLRPLHQLLEKTDIVLTPALAQLPLLIGHLRTDEEFNKYLQKKKEF
SPFTSLFNQGGLPAMTVPVMFHHHLPVSVQMGSAKGNDLLLLELAHELQSVLPKNITPASIP

Sequences:

>Translated_462_residues
MSMHVNEYIHYDVQELAELIKNHQVGAEEVLRCAQERLYQVNPSLNAVVTDCFEYAHQCLAKMKGNEPYYGVPLLVKDLG
HAIEGIRLTEGSRFFSNNLAETNSDLINKLIALGFVPFAKTNTSELGLSCVTDSNLFGPCRNPFDTMRTAGGSSGGSAAA
IMAGIAPVATASDGGGSIRIPAACCGLFGFKPTPGLVPSGPLVDEAWSGMATSFILTRSMSDAIALFKNVINPMRLNRLS
RKKKKLLITELTGAFAEVPIARECQEAVEKIKEILKNSGHRLNEVHLPLDLQVISSCAHTLIAASTYAKIKTQEQISGRK
PMIHELEPITWEFYYRGKMVSAYEFIIAKNKLYQSLRPLHQLLEKTDIVLTPALAQLPLLIGHLRTDEEFNKYLQKKKEF
SPFTSLFNQGGLPAMTVPVMFHHHLPVSVQMGSAKGNDLLLLELAHELQSVLPKNITPASIP
>Mature_461_residues
SMHVNEYIHYDVQELAELIKNHQVGAEEVLRCAQERLYQVNPSLNAVVTDCFEYAHQCLAKMKGNEPYYGVPLLVKDLGH
AIEGIRLTEGSRFFSNNLAETNSDLINKLIALGFVPFAKTNTSELGLSCVTDSNLFGPCRNPFDTMRTAGGSSGGSAAAI
MAGIAPVATASDGGGSIRIPAACCGLFGFKPTPGLVPSGPLVDEAWSGMATSFILTRSMSDAIALFKNVINPMRLNRLSR
KKKKLLITELTGAFAEVPIARECQEAVEKIKEILKNSGHRLNEVHLPLDLQVISSCAHTLIAASTYAKIKTQEQISGRKP
MIHELEPITWEFYYRGKMVSAYEFIIAKNKLYQSLRPLHQLLEKTDIVLTPALAQLPLLIGHLRTDEEFNKYLQKKKEFS
PFTSLFNQGGLPAMTVPVMFHHHLPVSVQMGSAKGNDLLLLELAHELQSVLPKNITPASIP

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=497, Percent_Identity=24.5472837022133, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI222831590, Length=511, Percent_Identity=23.4833659491194, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=192, Percent_Identity=34.8958333333333, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17537465, Length=199, Percent_Identity=31.1557788944724, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=447, Percent_Identity=25.7270693512304, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=306, Percent_Identity=28.4313725490196, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=311, Percent_Identity=27.0096463022508, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=295, Percent_Identity=29.8305084745763, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=306, Percent_Identity=29.0849673202614, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=306, Percent_Identity=29.0849673202614, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=396, Percent_Identity=26.010101010101, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320448, Length=238, Percent_Identity=30.2521008403361, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550774, Length=476, Percent_Identity=26.2605042016807, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648435, Length=476, Percent_Identity=26.2605042016807, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648437, Length=476, Percent_Identity=26.2605042016807, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356731, Length=487, Percent_Identity=26.0780287474333, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24644968, Length=238, Percent_Identity=32.7731092436975, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI161078093, Length=483, Percent_Identity=26.0869565217391, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24648441, Length=409, Percent_Identity=25.4278728606357, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648439, Length=409, Percent_Identity=25.4278728606357, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24652985, Length=482, Percent_Identity=25.3112033195021, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19922090, Length=482, Percent_Identity=25.3112033195021, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24652981, Length=482, Percent_Identity=25.3112033195021, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24652983, Length=482, Percent_Identity=25.3112033195021, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648113, Length=433, Percent_Identity=25.8660508083141, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 50783; Mature: 50651

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMHVNEYIHYDVQELAELIKNHQVGAEEVLRCAQERLYQVNPSLNAVVTDCFEYAHQCL
CCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AKMKGNEPYYGVPLLVKDLGHAIEGIRLTEGSRFFSNNLAETNSDLINKLIALGFVPFAK
HHHCCCCCCCCCHHHHHHHHHHHCCCEECCCCHHHHCCHHHCCHHHHHHHHHHHCCCCCC
TNTSELGLSCVTDSNLFGPCRNPFDTMRTAGGSSGGSAAAIMAGIAPVATASDGGGSIRI
CCCHHCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEH
PAACCGLFGFKPTPGLVPSGPLVDEAWSGMATSFILTRSMSDAIALFKNVINPMRLNRLS
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
RKKKKLLITELTGAFAEVPIARECQEAVEKIKEILKNSGHRLNEVHLPLDLQVISSCAHT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHH
LIAASTYAKIKTQEQISGRKPMIHELEPITWEFYYRGKMVSAYEFIIAKNKLYQSLRPLH
HHHHHHHHHHHCHHHHCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
QLLEKTDIVLTPALAQLPLLIGHLRTDEEFNKYLQKKKEFSPFTSLFNQGGLPAMTVPVM
HHHHHCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCEEEHHHH
FHHHLPVSVQMGSAKGNDLLLLELAHELQSVLPKNITPASIP
HCCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SMHVNEYIHYDVQELAELIKNHQVGAEEVLRCAQERLYQVNPSLNAVVTDCFEYAHQCL
CCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AKMKGNEPYYGVPLLVKDLGHAIEGIRLTEGSRFFSNNLAETNSDLINKLIALGFVPFAK
HHHCCCCCCCCCHHHHHHHHHHHCCCEECCCCHHHHCCHHHCCHHHHHHHHHHHCCCCCC
TNTSELGLSCVTDSNLFGPCRNPFDTMRTAGGSSGGSAAAIMAGIAPVATASDGGGSIRI
CCCHHCCCEEEECCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEH
PAACCGLFGFKPTPGLVPSGPLVDEAWSGMATSFILTRSMSDAIALFKNVINPMRLNRLS
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
RKKKKLLITELTGAFAEVPIARECQEAVEKIKEILKNSGHRLNEVHLPLDLQVISSCAHT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHH
LIAASTYAKIKTQEQISGRKPMIHELEPITWEFYYRGKMVSAYEFIIAKNKLYQSLRPLH
HHHHHHHHHHHCHHHHCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
QLLEKTDIVLTPALAQLPLLIGHLRTDEEFNKYLQKKKEFSPFTSLFNQGGLPAMTVPVM
HHHHHCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCEEEHHHH
FHHHLPVSVQMGSAKGNDLLLLELAHELQSVLPKNITPASIP
HCCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA