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The map label for this gene is cqsA [H]

Identifier: 52842935

GI number: 52842935

Start: 3080710

End: 3081960

Strand: Direct

Name: cqsA [H]

Synonym: lpg2731

Alternate gene names: 52842935

Gene position: 3080710-3081960 (Clockwise)

Preceding gene: 52842930

Following gene: 52842937

Centisome position: 90.67

GC content: 39.25

Gene sequence:

>1251_bases
ATGAGCAATACAGAATACCAAACAATTGATAACAAGACTCAATATATCAATGGAAAAGAAAAACATCAAAACCATGCTTA
TCCTGAGTACATCAATCATTCCATGGAAAAATATTACGAGGGACGAGTCAATAAATCCTGGCAAGGGAAACACATACTTA
AAGGCAAAACCCCGACTGCAAATTCACTGATTTTTTCGAGCAATGATTATTTAAACATCAGTCAACATCCGCAATTAATT
AAAGCGCAAATTGCTGCGATGCAAAAATATGGTAATGGTCAAATGCAATCTGCTGTATTTTTAAATAACAGCTCTTTTTT
GCTCAGCCAATGTGAAAAGAAATTCAGTTCCTTTTTAAATTATTCTGCGGCCCTGCTCACTCAATCAGGCTGGAGCGCTA
ACGTTGGTCTCATACAAACCCTGGCCAAGTACAATACACCAGTGTATCTGGATTTTTATGCCCATATGTCTTTTTGGTCT
GGTGTAAAAGCAGCAAGAGCCAAATCTATACCGTTTCTACATAATTCAGTCGATTCACTAAAAAAACGAATTGAGCACCA
TGGGCCAGGAATAATTGCAGTAGATTCTATTTATAGTACGCTGGGTACGATTAGTCCATTGGCTGACTATGCTGACATTG
CAAGAAAATTCAAATGTTTGCTCATCGTCGATGAATCTCATTCATTAGGTACGCATGGTTCGCAAGGCAAGGGGATGGTG
GCTAAACTGGGATTATCAAGCCAAATAGACATCATAACAGCAAGCCTGGCTAAAGCCTTATCAGGACGGGGAGGCATCAT
TACAGGAGATAGGCAACTTATAGAATATATCAGATATTCATCTCTACCCTCTATTTTTAGTTCTGCTTTAATGCCTCATG
ATTTAGCAGGATTTAGCGCATCATTAGAAATTATTACTCAAGAAGAATGGAGAAGAGCAAAATTACAAACCAATGCTGAT
TTTTTACGAGAAGCCTTATCATGCGAAGGAATTAATATAGGAAGTAGTGGCTCTCAAATAGTCCCTTTAGTTACAGGCAG
CGAGGCTAACACGCTTTGGTTAAGGGATGAGCTAGAAAAAGAAGACATCTTTGGAGCCGTGTTCTGTTCACCCGCCACTC
CCAAAAATAAATGCCTGATTCGTTTATCCATCAGCGCGCATCACGAAAAATCGGACTTGCTCAGAGTGATTGAGTGCCTG
GTCAGACTTGCAAGAAAAAGACCCGATATTCCTTTGTTTAAGGGTAATTAA

Upstream 100 bases:

>100_bases
TAAAATCATTAGCTGAATACTTAGACTTTTACTGAAAAGATCCTTCCACTATCAGCCATTGTGCTAAACTGCACACAAGG
GAAAAGAATCTGAGTTATGG

Downstream 100 bases:

>100_bases
CACGACTTCTAATTATTGGCGGCTCATTTGAACAGTTAACATTTCAAATAAGAATTATGTGCTCTTTATATAGAATTCCT
ATTAATTCAGAAACATTTCC

Product: hypothetical protein

Products: Acetyl-CoA; Glycine [C]

Alternate protein names: Cholerae quorum-sensing autoinducer [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSNTEYQTIDNKTQYINGKEKHQNHAYPEYINHSMEKYYEGRVNKSWQGKHILKGKTPTANSLIFSSNDYLNISQHPQLI
KAQIAAMQKYGNGQMQSAVFLNNSSFLLSQCEKKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWS
GVKAARAKSIPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLADYADIARKFKCLLIVDESHSLGTHGSQGKGMV
AKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEYIRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNAD
FLREALSCEGINIGSSGSQIVPLVTGSEANTLWLRDELEKEDIFGAVFCSPATPKNKCLIRLSISAHHEKSDLLRVIECL
VRLARKRPDIPLFKGN

Sequences:

>Translated_416_residues
MSNTEYQTIDNKTQYINGKEKHQNHAYPEYINHSMEKYYEGRVNKSWQGKHILKGKTPTANSLIFSSNDYLNISQHPQLI
KAQIAAMQKYGNGQMQSAVFLNNSSFLLSQCEKKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWS
GVKAARAKSIPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLADYADIARKFKCLLIVDESHSLGTHGSQGKGMV
AKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEYIRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNAD
FLREALSCEGINIGSSGSQIVPLVTGSEANTLWLRDELEKEDIFGAVFCSPATPKNKCLIRLSISAHHEKSDLLRVIECL
VRLARKRPDIPLFKGN
>Mature_415_residues
SNTEYQTIDNKTQYINGKEKHQNHAYPEYINHSMEKYYEGRVNKSWQGKHILKGKTPTANSLIFSSNDYLNISQHPQLIK
AQIAAMQKYGNGQMQSAVFLNNSSFLLSQCEKKFSSFLNYSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWSG
VKAARAKSIPFLHNSVDSLKKRIEHHGPGIIAVDSIYSTLGTISPLADYADIARKFKCLLIVDESHSLGTHGSQGKGMVA
KLGLSSQIDIITASLAKALSGRGGIITGDRQLIEYIRYSSLPSIFSSALMPHDLAGFSASLEIITQEEWRRAKLQTNADF
LREALSCEGINIGSSGSQIVPLVTGSEANTLWLRDELEKEDIFGAVFCSPATPKNKCLIRLSISAHHEKSDLLRVIECLV
RLARKRPDIPLFKGN

Specific function: Required for the synthesis of the quorum-sensing autoinducer CAI-1 ((S)-3-hydroxytridecan-4-one) which probably functions as an intragenus signal [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI83977444, Length=404, Percent_Identity=30.1980198019802, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI83977440, Length=404, Percent_Identity=30.1980198019802, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI7657118, Length=374, Percent_Identity=28.8770053475936, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI83977442, Length=404, Percent_Identity=30.1980198019802, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI284448556, Length=357, Percent_Identity=28.5714285714286, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI4502025, Length=333, Percent_Identity=29.4294294294294, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI40316939, Length=333, Percent_Identity=29.4294294294294, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI119220554, Length=311, Percent_Identity=27.0096463022508, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI4758668, Length=333, Percent_Identity=27.027027027027, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI5454084, Length=385, Percent_Identity=24.1558441558442, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1790046, Length=348, Percent_Identity=31.6091954022989, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786993, Length=342, Percent_Identity=26.0233918128655, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71994529, Length=372, Percent_Identity=28.7634408602151, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI32566772, Length=366, Percent_Identity=24.5901639344262, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560912, Length=360, Percent_Identity=25.8333333333333, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17560914, Length=358, Percent_Identity=25.9776536312849, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71982625, Length=245, Percent_Identity=25.3061224489796, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71982617, Length=245, Percent_Identity=25.3061224489796, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320438, Length=361, Percent_Identity=24.6537396121884, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320267, Length=352, Percent_Identity=25.2840909090909, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323954, Length=261, Percent_Identity=22.9885057471264, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24662918, Length=346, Percent_Identity=28.6127167630058, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI17137420, Length=365, Percent_Identity=28.7671232876712, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24653280, Length=265, Percent_Identity=25.6603773584906, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24653276, Length=265, Percent_Identity=25.6603773584906, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24653278, Length=265, Percent_Identity=25.6603773584906, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17136286, Length=324, Percent_Identity=25, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.3.1.29 [C]

Molecular weight: Translated: 46125; Mature: 45994

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTEYQTIDNKTQYINGKEKHQNHAYPEYINHSMEKYYEGRVNKSWQGKHILKGKTPTA
CCCCCCCCCCCCHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCC
NSLIFSSNDYLNISQHPQLIKAQIAAMQKYGNGQMQSAVFLNNSSFLLSQCEKKFSSFLN
CEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
YSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWSGVKAARAKSIPFLHNSVDSL
HHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KKRIEHHGPGIIAVDSIYSTLGTISPLADYADIARKFKCLLIVDESHSLGTHGSQGKGMV
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEE
AKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEYIRYSSLPSIFSSALMPHDLAGFSA
EECCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCHHHHHHHCCCCHHCCCCC
SLEIITQEEWRRAKLQTNADFLREALSCEGINIGSSGSQIVPLVTGSEANTLWLRDELEK
EEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEHHHCCH
EDIFGAVFCSPATPKNKCLIRLSISAHHEKSDLLRVIECLVRLARKRPDIPLFKGN
HHCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SNTEYQTIDNKTQYINGKEKHQNHAYPEYINHSMEKYYEGRVNKSWQGKHILKGKTPTA
CCCCCCCCCCCHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCC
NSLIFSSNDYLNISQHPQLIKAQIAAMQKYGNGQMQSAVFLNNSSFLLSQCEKKFSSFLN
CEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHH
YSAALLTQSGWSANVGLIQTLAKYNTPVYLDFYAHMSFWSGVKAARAKSIPFLHNSVDSL
HHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KKRIEHHGPGIIAVDSIYSTLGTISPLADYADIARKFKCLLIVDESHSLGTHGSQGKGMV
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCEE
AKLGLSSQIDIITASLAKALSGRGGIITGDRQLIEYIRYSSLPSIFSSALMPHDLAGFSA
EECCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCHHHHHHHCCCCHHCCCCC
SLEIITQEEWRRAKLQTNADFLREALSCEGINIGSSGSQIVPLVTGSEANTLWLRDELEK
EEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEHHHCCH
EDIFGAVFCSPATPKNKCLIRLSISAHHEKSDLLRVIECLVRLARKRPDIPLFKGN
HHCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5-phosphate mol per mol subunit, enzyme, [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 12 {butyryl-CoA}} 0.08 {propionyl-CoA}} 0.059 {acetyl-CoA}} [C]

Substrates: L-2-Amino-3-oxobutanoate; Coenzyme A [C]

Specific reaction: L-2-Amino-3-oxobutanoate + Coenzyme A --> Acetyl-CoA + Glycine [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 2-Cyclohexanedione; 2, 3-Butanedione; 4-(Oxoacetyl) phenoxyacetic acid; Aminomalonic acid; Aminomethyl phosphonic acid; Aminooxyacetate; Ba2+; Ca2+; Cd2+; CoA; Cu2+; DTT; Glutathione; Hg2+; Hydroxy l amine; IAA; L-Cysteine; Mn2+; PCMB, NEM, glycinemeth

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301; 12176318 [H]