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The map label for this gene is tnpA [H]

Identifier: 52842330

GI number: 52842330

Start: 2361172

End: 2362620

Strand: Direct

Name: tnpA [H]

Synonym: lpg2116

Alternate gene names: 52842330

Gene position: 2361172-2362620 (Clockwise)

Preceding gene: 52842328

Following gene: 52842334

Centisome position: 69.49

GC content: 38.58

Gene sequence:

>1449_bases
ATGATCAAATCACCTCTTTTTGAGGTATGTATGGACTTAGCAGTTGAAGATACTACAGCATGGTCGGAAGCTATTTTTGG
TTCAGTTGCTTTAGGGGATAAACGACTTACTCGTCGGTTAATTCAAATAGGCAAACAATTATCATCGACGCCTGGTGGTT
CTCTTTCAGGAAGTTGTGGAGGGCAGGATGCGCTTATAGAAGGTAGTTATCGTTTTTTACGAAACAAACGAGTCACAGCG
AATCAAATTGCAGAGGGTGGTTATCAAGTAACAAGCTGGTTATCTCAGTCAATCCCCACGCTTTTAGCCATTGAAGACAC
AACCACGCTCTCCTATACCCATCAAGTAAAAGAATCATTAGGTGATTTAGGCGGTCCTAAAGAAAAAAGCAATCGAGGTT
TTCATGTTCACACCACCATGCTCATGGATGCAGAACAAGAGAAAACAATAGGATTAATCGCGCAAGAACGTTGGTGTCGA
GATATCAAAGAAAGAGGCAAAAAAAATCATCGACGAGTAAGGTTATATACAGAGAAAGAAAGCTATAAATGGGAAAAGAA
TACTCGGGAATTAGAGAATCGATTGGGTTCTAAAATGTCTGATGTGATTTCTGTTTGCGATAGAGAAGCTGATATTTTTG
AATACATTCAGTACAAGTTAGACCATGCTCAACGTTTTATTGTTCGAGCGAGCCATAATCGAAAACTAGAAGGAAGTAAC
TGTTATTTATTTCAAATGTTACCTTCAGCAGTAAACTTGGGTATTTATACAATTGAAGTGGCTCAAAAAGCAAATAGAAA
GAAACGCCAAGTGACTCTTGAGTTAAAAACAACATCTGTTACTTTCTCACCTTCTGAACGAAGAGCTAAAGCACGTGAAT
TAAAACCAATCACTTTAAACGTAGTAATCGCTAAAGAGAAAAATCCATCTGAAAGCGATTGCCTTGAATGGATTCTATTA
ACAACAGAAGCCACAACAACATTAGAGTGTACCCGAAAAATAACGCGATATTATGAAATGAGATGGCGCATTGAGGATTT
TCATAAAGCATGGAAATCGGGGGTTGGTGCTGAAGAGCAGCGTATGCAATCAATTGAAAATTTAGAGAAAATGATTGTCA
TTCTCTCATTTGTAGCAATTCGATTACTCCAATTAAAAGAGTATTTTGAATACCCGACTACTCTAGTTATCAATGATAGC
AGTACTTCTTGCGATGAATTGCTCACTGATGCTGAATGGAAAGTCTTATGGAATAGTGTTGAAAGAAAATCATTACCTGA
AAAAATACCTACTGCTGCTTGGGCTTATAAAGCAATAGCCAAGTTGGGCGGTTGGACTGATTCCAAAAGAACTGGGAAAG
CAGCTTGGTCTACTATCTGGAAAGGATGGTTCCGATTACAGGAACGAGTAGAAGGGCTTAGGATAGCTAACGAGTTGATG
GAGATGTGA

Upstream 100 bases:

>100_bases
CGCGCCATTTGGGTCTGGTCTATTCCGAAGACATCGGTTACAAATTGGGTCTTTCCCAAATACCTGAATCTTCTACACAT
CTTGTTTATTCCAAACAAAC

Downstream 100 bases:

>100_bases
TCAAGAGACAGCCCAAATACGGGGGCATTTTATCAAGCACATAATACTCTAACTCTTAATCGGTAGTTTTCAAAGTTTCT
AAACCCATAAGCTCTTCGTT

Product: IS4 family transposase TnpA

Products: NA

Alternate protein names: Tnp [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MIKSPLFEVCMDLAVEDTTAWSEAIFGSVALGDKRLTRRLIQIGKQLSSTPGGSLSGSCGGQDALIEGSYRFLRNKRVTA
NQIAEGGYQVTSWLSQSIPTLLAIEDTTTLSYTHQVKESLGDLGGPKEKSNRGFHVHTTMLMDAEQEKTIGLIAQERWCR
DIKERGKKNHRRVRLYTEKESYKWEKNTRELENRLGSKMSDVISVCDREADIFEYIQYKLDHAQRFIVRASHNRKLEGSN
CYLFQMLPSAVNLGIYTIEVAQKANRKKRQVTLELKTTSVTFSPSERRAKARELKPITLNVVIAKEKNPSESDCLEWILL
TTEATTTLECTRKITRYYEMRWRIEDFHKAWKSGVGAEEQRMQSIENLEKMIVILSFVAIRLLQLKEYFEYPTTLVINDS
STSCDELLTDAEWKVLWNSVERKSLPEKIPTAAWAYKAIAKLGGWTDSKRTGKAAWSTIWKGWFRLQERVEGLRIANELM
EM

Sequences:

>Translated_482_residues
MIKSPLFEVCMDLAVEDTTAWSEAIFGSVALGDKRLTRRLIQIGKQLSSTPGGSLSGSCGGQDALIEGSYRFLRNKRVTA
NQIAEGGYQVTSWLSQSIPTLLAIEDTTTLSYTHQVKESLGDLGGPKEKSNRGFHVHTTMLMDAEQEKTIGLIAQERWCR
DIKERGKKNHRRVRLYTEKESYKWEKNTRELENRLGSKMSDVISVCDREADIFEYIQYKLDHAQRFIVRASHNRKLEGSN
CYLFQMLPSAVNLGIYTIEVAQKANRKKRQVTLELKTTSVTFSPSERRAKARELKPITLNVVIAKEKNPSESDCLEWILL
TTEATTTLECTRKITRYYEMRWRIEDFHKAWKSGVGAEEQRMQSIENLEKMIVILSFVAIRLLQLKEYFEYPTTLVINDS
STSCDELLTDAEWKVLWNSVERKSLPEKIPTAAWAYKAIAKLGGWTDSKRTGKAAWSTIWKGWFRLQERVEGLRIANELM
EM
>Mature_482_residues
MIKSPLFEVCMDLAVEDTTAWSEAIFGSVALGDKRLTRRLIQIGKQLSSTPGGSLSGSCGGQDALIEGSYRFLRNKRVTA
NQIAEGGYQVTSWLSQSIPTLLAIEDTTTLSYTHQVKESLGDLGGPKEKSNRGFHVHTTMLMDAEQEKTIGLIAQERWCR
DIKERGKKNHRRVRLYTEKESYKWEKNTRELENRLGSKMSDVISVCDREADIFEYIQYKLDHAQRFIVRASHNRKLEGSN
CYLFQMLPSAVNLGIYTIEVAQKANRKKRQVTLELKTTSVTFSPSERRAKARELKPITLNVVIAKEKNPSESDCLEWILL
TTEATTTLECTRKITRYYEMRWRIEDFHKAWKSGVGAEEQRMQSIENLEKMIVILSFVAIRLLQLKEYFEYPTTLVINDS
STSCDELLTDAEWKVLWNSVERKSLPEKIPTAAWAYKAIAKLGGWTDSKRTGKAAWSTIWKGWFRLQERVEGLRIANELM
EM

Specific function: Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After n

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559
- InterPro:   IPR003201
- InterPro:   IPR014737
- InterPro:   IPR014736
- InterPro:   IPR014735 [H]

Pfam domain/function: PF01609 Transposase_11; PF02281 Transposase_Tn5 [H]

EC number: NA

Molecular weight: Translated: 55307; Mature: 55307

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSPLFEVCMDLAVEDTTAWSEAIFGSVALGDKRLTRRLIQIGKQLSSTPGGSLSGSCG
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GQDALIEGSYRFLRNKRVTANQIAEGGYQVTSWLSQSIPTLLAIEDTTTLSYTHQVKESL
CCCCEECCHHHHHHCCCCCHHHHHCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
GDLGGPKEKSNRGFHVHTTMLMDAEQEKTIGLIAQERWCRDIKERGKKNHRRVRLYTEKE
HCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
SYKWEKNTRELENRLGSKMSDVISVCDREADIFEYIQYKLDHAQRFIVRASHNRKLEGSN
CCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CYLFQMLPSAVNLGIYTIEVAQKANRKKRQVTLELKTTSVTFSPSERRAKARELKPITLN
EEEEEECHHHHCCCEEEEEHHHHCCCCHHEEEEEEEEEEEEECCHHHHHHHHCCCCEEEE
VVIAKEKNPSESDCLEWILLTTEATTTLECTRKITRYYEMRWRIEDFHKAWKSGVGAEEQ
EEEEECCCCCHHHHHEEHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RMQSIENLEKMIVILSFVAIRLLQLKEYFEYPTTLVINDSSTSCDELLTDAEWKVLWNSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCHHHHHHHHH
ERKSLPEKIPTAAWAYKAIAKLGGWTDSKRTGKAAWSTIWKGWFRLQERVEGLRIANELM
HHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EM
CC
>Mature Secondary Structure
MIKSPLFEVCMDLAVEDTTAWSEAIFGSVALGDKRLTRRLIQIGKQLSSTPGGSLSGSCG
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GQDALIEGSYRFLRNKRVTANQIAEGGYQVTSWLSQSIPTLLAIEDTTTLSYTHQVKESL
CCCCEECCHHHHHHCCCCCHHHHHCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
GDLGGPKEKSNRGFHVHTTMLMDAEQEKTIGLIAQERWCRDIKERGKKNHRRVRLYTEKE
HCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
SYKWEKNTRELENRLGSKMSDVISVCDREADIFEYIQYKLDHAQRFIVRASHNRKLEGSN
CCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CYLFQMLPSAVNLGIYTIEVAQKANRKKRQVTLELKTTSVTFSPSERRAKARELKPITLN
EEEEEECHHHHCCCEEEEEHHHHCCCCHHEEEEEEEEEEEEECCHHHHHHHHCCCCEEEE
VVIAKEKNPSESDCLEWILLTTEATTTLECTRKITRYYEMRWRIEDFHKAWKSGVGAEEQ
EEEEECCCCCHHHHHEEHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RMQSIENLEKMIVILSFVAIRLLQLKEYFEYPTTLVINDSSTSCDELLTDAEWKVLWNSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCHHHHHHHHH
ERKSLPEKIPTAAWAYKAIAKLGGWTDSKRTGKAAWSTIWKGWFRLQERVEGLRIANELM
HHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EM
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6260374; 6271452; 7867940; 6291786; 10884228; 12367522; 11896402 [H]