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The map label for this gene is carA [H]

Identifier: 52842240

GI number: 52842240

Start: 2263582

End: 2264706

Strand: Reverse

Name: carA [H]

Synonym: lpg2023

Alternate gene names: 52842240

Gene position: 2264706-2263582 (Counterclockwise)

Preceding gene: 52842241

Following gene: 52842239

Centisome position: 66.65

GC content: 40.62

Gene sequence:

>1125_bases
TTGAGTATATTCATGATTAATAAATCAGCAATTTTAGCTTTAGCTGATGGCACTGTTTATGAGGGCCTGTCAGTAGGGGC
CAGTGGTGATTCTGTTGGTGAGTTGGTTTTTAACACGTCAATGACTGGCTATCAGGAAATGCTTACTGATCCTTCGTATG
CCAGACAAATAATTACCTTAACTACTGCTCATGTGGGAAATACCGGTTGTAACATGGAGGACATGGAGTCCACAAAAGTC
TGGGCAGCCGGCCTCGTCATGAGAAACTGCACTGCAATCCCTAGCAATTACAGAGCTGAACAATCCTTCCCAGACTGGTT
AAGTAAAAATGGAGTAGTTGCAATTTCTGGCATTGATACGCGCGCGCTTACACTGAAATTGCGTGAGAAGGGTTCTATCG
GAGCCTGTATTAGCACCAATGTGGAGAAACCTGAAATTGTTGTGGCAAAGGCTAAATCCTTTGCAGGGCTTCAGGGTGCA
GATTTGGCTTTAGAGGTTACAAGACAAACGATAGAGCGTTGGCATGAAGGTCGGGGAATGTGGAGTCTAGACAAACAGCC
TCAACAGCTTCATGTTGTTGCCTATGATTTTGGTGTAAAACATAATATTTTACGTATCTTACATGATAAGGGATGCCATA
TTACTATAGTCCCTGCTAAAACTTCTGCAGAAGAGGTTTTAGCCATGAATCCTAATGGTGTCTTTTTATCCAATGGACCG
GGTGATCCCCAAGCATGCGATTATGCCATCAAAGCAACACAGGTGTTTTTAGACAATAATATTCCTTTATTTGGTATATG
CCTTGGGTTTCAGATTTTGGCTTTGGCATGTGGTGGTATTACTAAAAAAATGAAATTTGGACATCATGGTTCCAATCATC
CTGTAGTTGAAACAACTGGCAAAAAACGTGTTTTTATAACAAGTCAAAATCATGGTTTTGCTGTAGATGAGGATTCATTG
CCCAATTGCCTGGAAGTTACGCATCGTTCATTATTTGATAACAGTTTACAGGGAATAAGGCACAAAACAAAACCGGCATT
TGGTTTTCAAGGCCATCCAGAAGCAAGCCCTGGGCCACATGATATAGATATTTTATTTAATGAATTTTTAAATCTGATGA
AATAA

Upstream 100 bases:

>100_bases
AAGACATGAAATTTTGACTTAATTAAGTTACAATTGGAAGATTTGTCGTTTTCAGCATGGATTGCCTATTTTACGCTGGA
TAAGCATACTTTGAACATAA

Downstream 100 bases:

>100_bases
ATTTTTAGCTTCAGAATTATTATTTTGGCAAGAGTGGCTTGTTTTAAGCCTGCTTACAATGATTGTAATACCCCTAAATG
CTATCCATGGCTATTAAAGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSIFMINKSAILALADGTVYEGLSVGASGDSVGELVFNTSMTGYQEMLTDPSYARQIITLTTAHVGNTGCNMEDMESTKV
WAAGLVMRNCTAIPSNYRAEQSFPDWLSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIVVAKAKSFAGLQGA
DLALEVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTSAEEVLAMNPNGVFLSNGP
GDPQACDYAIKATQVFLDNNIPLFGICLGFQILALACGGITKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSL
PNCLEVTHRSLFDNSLQGIRHKTKPAFGFQGHPEASPGPHDIDILFNEFLNLMK

Sequences:

>Translated_374_residues
MSIFMINKSAILALADGTVYEGLSVGASGDSVGELVFNTSMTGYQEMLTDPSYARQIITLTTAHVGNTGCNMEDMESTKV
WAAGLVMRNCTAIPSNYRAEQSFPDWLSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIVVAKAKSFAGLQGA
DLALEVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTSAEEVLAMNPNGVFLSNGP
GDPQACDYAIKATQVFLDNNIPLFGICLGFQILALACGGITKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSL
PNCLEVTHRSLFDNSLQGIRHKTKPAFGFQGHPEASPGPHDIDILFNEFLNLMK
>Mature_373_residues
SIFMINKSAILALADGTVYEGLSVGASGDSVGELVFNTSMTGYQEMLTDPSYARQIITLTTAHVGNTGCNMEDMESTKVW
AAGLVMRNCTAIPSNYRAEQSFPDWLSKNGVVAISGIDTRALTLKLREKGSIGACISTNVEKPEIVVAKAKSFAGLQGAD
LALEVTRQTIERWHEGRGMWSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTSAEEVLAMNPNGVFLSNGPG
DPQACDYAIKATQVFLDNNIPLFGICLGFQILALACGGITKKMKFGHHGSNHPVVETTGKKRVFITSQNHGFAVDEDSLP
NCLEVTHRSLFDNSLQGIRHKTKPAFGFQGHPEASPGPHDIDILFNEFLNLMK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=376, Percent_Identity=37.7659574468085, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI21361331, Length=379, Percent_Identity=32.9815303430079, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI169790915, Length=379, Percent_Identity=32.9815303430079, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=58.0901856763926, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1789760, Length=136, Percent_Identity=28.6764705882353, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=379, Percent_Identity=35.8839050131926, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=34.5177664974619, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324878, Length=378, Percent_Identity=37.037037037037, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322638, Length=177, Percent_Identity=28.2485875706215, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45555749, Length=386, Percent_Identity=36.7875647668394, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24642586, Length=386, Percent_Identity=36.7875647668394, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40670; Mature: 40539

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFMINKSAILALADGTVYEGLSVGASGDSVGELVFNTSMTGYQEMLTDPSYARQIITL
CEEEEEECCEEEEEECCCEECCEEECCCCCCCCCEEEECCCCCHHHHHCCHHHHHEEEEE
TTAHVGNTGCNMEDMESTKVWAAGLVMRNCTAIPSNYRAEQSFPDWLSKNGVVAISGIDT
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEEEEECCCC
RALTLKLREKGSIGACISTNVEKPEIVVAKAKSFAGLQGADLALEVTRQTIERWHEGRGM
EEEEEEEECCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
WSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTSAEEVLAMNPNGVFLSNGP
CCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEECCC
GDPQACDYAIKATQVFLDNNIPLFGICLGFQILALACGGITKKMKFGHHGSNHPVVETTG
CCHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEECC
KKRVFITSQNHGFAVDEDSLPNCLEVTHRSLFDNSLQGIRHKTKPAFGFQGHPEASPGPH
CEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
DIDILFNEFLNLMK
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SIFMINKSAILALADGTVYEGLSVGASGDSVGELVFNTSMTGYQEMLTDPSYARQIITL
EEEEEECCEEEEEECCCEECCEEECCCCCCCCCEEEECCCCCHHHHHCCHHHHHEEEEE
TTAHVGNTGCNMEDMESTKVWAAGLVMRNCTAIPSNYRAEQSFPDWLSKNGVVAISGIDT
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCEEEEECCCC
RALTLKLREKGSIGACISTNVEKPEIVVAKAKSFAGLQGADLALEVTRQTIERWHEGRGM
EEEEEEEECCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
WSLDKQPQQLHVVAYDFGVKHNILRILHDKGCHITIVPAKTSAEEVLAMNPNGVFLSNGP
CCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEECCC
GDPQACDYAIKATQVFLDNNIPLFGICLGFQILALACGGITKKMKFGHHGSNHPVVETTG
CCHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEECC
KKRVFITSQNHGFAVDEDSLPNCLEVTHRSLFDNSLQGIRHKTKPAFGFQGHPEASPGPH
CEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
DIDILFNEFLNLMK
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA