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The map label for this gene is metK

Identifier: 52842239

GI number: 52842239

Start: 2262225

End: 2263373

Strand: Reverse

Name: metK

Synonym: lpg2022

Alternate gene names: 52842239

Gene position: 2263373-2262225 (Counterclockwise)

Preceding gene: 52842240

Following gene: 52842238

Centisome position: 66.61

GC content: 42.38

Gene sequence:

>1149_bases
ATGAATGAAGTCTATGTTTTTACCTCAGAGTCTGTTTCCGAGGGCCATCCTGATAAGATAGCTGATCAAATTTCAGATGC
CATTTTAGATGCTATTTTAGCCCAAGATCCAAAAGCACGAGTGGCTTGTGAGGTATTGGTTAAAACCGGCATGGTTTTGG
TTGGTGGAGAAATTACGACGAAAGCTTGGGTTGATGTTGAAGAAATAACTCGTCATGTTATCAAGGACATTGGCTATAAT
AGTTCCCAGATGGGGTTTGATTGGGAATCTTGTGCTGTGCTTTCAGCGATTGGCAAGCAATCACCAGACATAGCTCAAGG
TGTCGATAATCAGCAAACTAAAATTCTGGGTGCTGGCGATCAGGGACTTATGTTTGGTTATGCCAGCCGTGAAACAGATG
TTTTTATGCCTGCCCCTATTGCCTATGCGCATCGTTTGATGGAGAAATTAGCTAAAGCTCGTAAATCAGGCCAATTACCC
TGGTTAAGACCTGATGCAAAATGTCAACTTACTTTGAAATATGAACAAGGGATGCCGGTTGAAGTGGATACTGTTGTTTT
TTCTACGCAGCATTCACCTGATATTGAGCATAAAGATTTGGTTGAGGCGATCCGTGAAGAAATTATTAAATCAGTTCTTC
CTGCTGAATGGCTCAATGATAAGACCCGGTATTTCATTAATCCGACAGGACGCTTCGTAATTGGTGGTCCTTTGGGCGAT
TGTGGCTTAACCGGCCGTAAAATCATTGTTGATACTTATGGTGGTATGGCAAGACATGGCGGAGGCTGTTTTTCAGGTAA
AGATCCTTCGAAGGTAGATCGCTCCGCAGCATACGCCGCAAGACATGTTGCCAAGAATATTGTGGCGGCTGGTTTGGCGG
ATAAATGCGAGTTACAGATTTCCTATGCTATTGGTGTTGCTGAACCTACATCTATTTTTGTTGATACTTTTGGTACTGGA
CGACTAAAAAACAGCGAAATAATTGATTTAATTCATACGCATTTTGATTTAACACCGCAAGGTATTATCGACCAACACGA
TTTGCTTCGTCCTATTTATAGGCAGACGGCAACTTATGGGCACTATGGCAGGGAGAGTTTTCCATGGGAGCGATTGGACA
AAGTAGCTGAATTAAGCAAGGCATTATAA

Upstream 100 bases:

>100_bases
ATGGCATGGATTTACCAAAACAATGACTTTAACTTGTTTTGTGTTTTAAGCAGAGATAAAGATTAGGGATACTAAAAATA
GAAAATACAGAGGATATGTA

Downstream 100 bases:

>100_bases
TTTTTTAAGGATACTCTAATGGAACTGGTAGAAGAAGGAGTACAGAAAATCAATACATTACAAGATTATAAAGTAGCAGA
GATTTCATTAGCGGACTGGG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITTKAWVDVEEITRHVIKDIGYN
SSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTG
RLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYGHYGRESFPWERLDKVAELSKAL

Sequences:

>Translated_382_residues
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITTKAWVDVEEITRHVIKDIGYN
SSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTG
RLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYGHYGRESFPWERLDKVAELSKAL
>Mature_382_residues
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITTKAWVDVEEITRHVIKDIGYN
SSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGDQGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLP
WLRPDAKCQLTLKYEQGMPVEVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQISYAIGVAEPTSIFVDTFGTG
RLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYGHYGRESFPWERLDKVAELSKAL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=56.7204301075269, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI5174529, Length=371, Percent_Identity=55.256064690027, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI1789311, Length=377, Percent_Identity=71.8832891246684, Blast_Score=572, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI17538498, Length=372, Percent_Identity=57.258064516129, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17538494, Length=372, Percent_Identity=56.989247311828, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25145633, Length=376, Percent_Identity=55.5851063829787, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=54.5219638242894, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32565905, Length=372, Percent_Identity=51.0752688172043, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=54.601226993865, Blast_Score=348, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI25145635, Length=330, Percent_Identity=53.3333333333333, Blast_Score=340, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=53.8461538461538, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=64.6341463414634, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=69.5652173913043, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=376, Percent_Identity=56.3829787234042, Blast_Score=418, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=55.4089709762533, Blast_Score=416, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580527, Length=372, Percent_Identity=54.5698924731183, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580525, Length=372, Percent_Identity=54.5698924731183, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI45552159, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=372, Percent_Identity=54.5698924731183, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580541, Length=358, Percent_Identity=53.9106145251397, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=57.9831932773109, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41979; Mature: 41979

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITT
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCH
KAWVDVEEITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGD
HHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCEEEEECCC
QGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLPWLRPDAKCQLTLKYEQGMPV
CCEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCE
EVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQI
CCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYG
EEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
HYGRESFPWERLDKVAELSKAL
CCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNEVYVFTSESVSEGHPDKIADQISDAILDAILAQDPKARVACEVLVKTGMVLVGGEITT
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCH
KAWVDVEEITRHVIKDIGYNSSQMGFDWESCAVLSAIGKQSPDIAQGVDNQQTKILGAGD
HHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCEEEEECCC
QGLMFGYASRETDVFMPAPIAYAHRLMEKLAKARKSGQLPWLRPDAKCQLTLKYEQGMPV
CCEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCE
EVDTVVFSTQHSPDIEHKDLVEAIREEIIKSVLPAEWLNDKTRYFINPTGRFVIGGPLGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCCEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGCFSGKDPSKVDRSAAYAARHVAKNIVAAGLADKCELQI
CCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSIFVDTFGTGRLKNSEIIDLIHTHFDLTPQGIIDQHDLLRPIYRQTATYG
EEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
HYGRESFPWERLDKVAELSKAL
CCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA