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The map label for this gene is rph

Identifier: 52842229

GI number: 52842229

Start: 2252185

End: 2252892

Strand: Direct

Name: rph

Synonym: lpg2012

Alternate gene names: 52842229

Gene position: 2252185-2252892 (Clockwise)

Preceding gene: 52842216

Following gene: 52842231

Centisome position: 66.28

GC content: 41.24

Gene sequence:

>708_bases
ATGCGTCCCAGTAATCGTGAACATGATCAACTTCGTCCCGTTACAATAACACGTAATTTTACCAATTATGCCGAAGGCTC
TGTATTAGTCGAATTTGGTCAGACAAAAGTGATTTGTAACGCATCAATCGTCGAAGGAGTTCCCCGGTTTTTAAAAGGTA
AAAACCAAGGGTGGATAACAGCTGAATATGGCATGCTCCCACGTGCAACTCATAGCCGTACCGAAAGGGAAGCCAGTAAA
GGTAAGCAAGGTGGCAGAACATTGGAAATTCAACGTCTCATAGGGCGTTCATTGAGAGCATGTATCGATTTAAAGGTTTT
GGGCGAAAATACTATTACTCTTGATTGTGATGTTATTCAAGCGGATGGAGGAACCAGAACAGCAGCTATAACTGGCAGTT
GTGTGGCTATGCGAGATGCAATTCACTGGATGGTTCAACGTGAAAAAATAAAGAAGATGCCTGCTTTTAATTATGTAGCT
GCCGTATCTGTAGGTATTTACAGGGGTCAACCCGTGCTGGATTTGGATTATGCAGAAGATGTTCTTGCTGAAACAGATAT
GAATGTGGTTATGAATGAACAAGGACATTTTATTGAAGTTCAAGGCACTGCTGAAGATAACTCCTTTAACCGGGAACAAC
TGAACAGCATGTTGTCATTGGCTGAAATTGGTATTCCTCAATTGATTGAAATCCAAAAAAATGCCTGA

Upstream 100 bases:

>100_bases
CAGCAAAAATAGAGAGGAACGTACAAATAGCGTATGTATTTAGGGTGCAACTCAAGTATTAAAGAGTTATACTTGGGCAT
TTTACAGAGGAGTATCTCTA

Downstream 100 bases:

>100_bases
GAACAATAGCCCGGCATAAACAGTCGCCCGCTATAGAAATTAATACTTCAAACCTGAGCAAAAGATCTCTGCAGGAATGC
TTTGAGCTAACTGAAGAGAT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIVEGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASK
GKQGGRTLEIQRLIGRSLRACIDLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPAFNYVA
AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSMLSLAEIGIPQLIEIQKNA

Sequences:

>Translated_235_residues
MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIVEGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASK
GKQGGRTLEIQRLIGRSLRACIDLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPAFNYVA
AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSMLSLAEIGIPQLIEIQKNA
>Mature_235_residues
MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIVEGVPRFLKGKNQGWITAEYGMLPRATHSRTEREASK
GKQGGRTLEIQRLIGRSLRACIDLKVLGENTITLDCDVIQADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPAFNYVA
AVSVGIYRGQPVLDLDYAEDVLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSMLSLAEIGIPQLIEIQKNA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=69.8564593301435, Blast_Score=307, Evalue=4e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_LEGPA (Q5X3P0)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_124308.1
- ProteinModelPortal:   Q5X3P0
- SMR:   Q5X3P0
- STRING:   Q5X3P0
- GeneID:   3118727
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp1994
- LegioList:   lpp1994
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   LPNE297246:LPP1994-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26110; Mature: 26110

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIVEGVPRFLKGKNQGWIT
CCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHCCCCCCEEE
AEYGMLPRATHSRTEREASKGKQGGRTLEIQRLIGRSLRACIDLKVLGENTITLDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHEEEEEECCCEEEEEEEEEE
ADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPAFNYVAAVSVGIYRGQPVLDLDYAED
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEECCCCEEECCHHHH
VLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSMLSLAEIGIPQLIEIQKNA
HHHHCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure
MRPSNREHDQLRPVTITRNFTNYAEGSVLVEFGQTKVICNASIVEGVPRFLKGKNQGWIT
CCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHCCCCCCEEE
AEYGMLPRATHSRTEREASKGKQGGRTLEIQRLIGRSLRACIDLKVLGENTITLDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHEEEEEECCCEEEEEEEEEE
ADGGTRTAAITGSCVAMRDAIHWMVQREKIKKMPAFNYVAAVSVGIYRGQPVLDLDYAED
CCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEECCCCEEECCHHHH
VLAETDMNVVMNEQGHFIEVQGTAEDNSFNREQLNSMLSLAEIGIPQLIEIQKNA
HHHHCCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA