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The map label for this gene is queA

Identifier: 52842221

GI number: 52842221

Start: 2243419

End: 2244468

Strand: Reverse

Name: queA

Synonym: lpg2004

Alternate gene names: 52842221

Gene position: 2244468-2243419 (Counterclockwise)

Preceding gene: 52842222

Following gene: 52842220

Centisome position: 66.06

GC content: 40.48

Gene sequence:

>1050_bases
GTGTATAATTGCACCAATTTGTTTTTAGGTTGTTCTATGAATAAGCAAGATTTTTATTTTGATTTGCCTTCCGAATTAAT
AGCCCAATATCCTCTGGCGAATAGAAGTGATTCACGGCTTTTAATTTATAATCGACAGACAGAAGAATATGGCCATTATC
AATTCCGGGAAATTGCAGATTTCTTACAGCCAGGGGACTTATTGGTAATGAATGACAGCAAAGTGATACCAGCACGCTTA
TACGGCCACAAAGCCACTGGCGGTAAGGTTGAACTGTTAGTAGAGAGGATAACTGGCGATTTTACTTTCCTGGCCCATAT
CAAAGCGAGCAAATCATTAAAATCAAACGATCTCATTTATCTTGATGCTGGTAAGCGGCTTGAAGTATTACAAAGGCAGG
ATGATTTATTTCTTTGTAAGGCGTGCGAAAATATTTTAGATCTTTTAAATGATCTTGGGCATATTCCTTTACCACCTTAT
ATCGCCAGAGAAGATGAATCACTGGATAAGGAGAGATATCAAACCGTCTATGCCAAGTGCGCGGGGTCTGTTGCTGCGCC
TACAGCAGGATTGCATTTTGATGATGCTGTTTTATCCAGTATTAGAGCGCGAGGAGTTAATATTGCTTATGTCACTCTCC
ATGTAGGGGCAGGAACATTCAGGCCAGTTCGCTGTGAACGCATTCAGGATCATAAAATGCATAGTGAGTGGTTTACGGTT
AGTCCTGATCTGTGCACTGCAGTGAAGGCTGCCAAATCTATGGGAAATAGAGTAATTGCTGTTGGGACTACTGCATTGCG
CAGCCTTGAAAGCGCTGCTATGGGAGGAGAGCTAATTCCATGTAGTCGTGATACCGATATTTTCATTTATCCTGGCTACC
AGTTTAAAGTTTGTGATGGATTAATAACTAATTTTCATTTGCCTGAATCAACTCTGGTGATGTTGGTGTCGGCTTTTATT
GGGCACCAGGAGTGTATGGCTCTTTATCAAGAAGCAATCGATAAAAGGTATCGTTTTTTTAGTTATGGAGATGCCAGTTT
ATTGTTATAA

Upstream 100 bases:

>100_bases
TCTTGCTCGTAGTCTCTTAAACAGATAAAAAAAACAAATGATGTTAATTTTCTAATAAAATGAATAAATTCCTGATAATT
GATTTTAAGACTAAAGTTCG

Downstream 100 bases:

>100_bases
AGTGCTCTGGAATATAATAATTTTGGCTACAGTTAGAGGGTAGTGGATGTTGAGTACAAATCAAAATTTTGTTCCTGTTT
TAAATACTGAAGCAGGACTA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MYNCTNLFLGCSMNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFREIADFLQPGDLLVMNDSKVIPARL
YGHKATGGKVELLVERITGDFTFLAHIKASKSLKSNDLIYLDAGKRLEVLQRQDDLFLCKACENILDLLNDLGHIPLPPY
IAREDESLDKERYQTVYAKCAGSVAAPTAGLHFDDAVLSSIRARGVNIAYVTLHVGAGTFRPVRCERIQDHKMHSEWFTV
SPDLCTAVKAAKSMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIFIYPGYQFKVCDGLITNFHLPESTLVMLVSAFI
GHQECMALYQEAIDKRYRFFSYGDASLLL

Sequences:

>Translated_349_residues
MYNCTNLFLGCSMNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFREIADFLQPGDLLVMNDSKVIPARL
YGHKATGGKVELLVERITGDFTFLAHIKASKSLKSNDLIYLDAGKRLEVLQRQDDLFLCKACENILDLLNDLGHIPLPPY
IAREDESLDKERYQTVYAKCAGSVAAPTAGLHFDDAVLSSIRARGVNIAYVTLHVGAGTFRPVRCERIQDHKMHSEWFTV
SPDLCTAVKAAKSMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIFIYPGYQFKVCDGLITNFHLPESTLVMLVSAFI
GHQECMALYQEAIDKRYRFFSYGDASLLL
>Mature_349_residues
MYNCTNLFLGCSMNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFREIADFLQPGDLLVMNDSKVIPARL
YGHKATGGKVELLVERITGDFTFLAHIKASKSLKSNDLIYLDAGKRLEVLQRQDDLFLCKACENILDLLNDLGHIPLPPY
IAREDESLDKERYQTVYAKCAGSVAAPTAGLHFDDAVLSSIRARGVNIAYVTLHVGAGTFRPVRCERIQDHKMHSEWFTV
SPDLCTAVKAAKSMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIFIYPGYQFKVCDGLITNFHLPESTLVMLVSAFI
GHQECMALYQEAIDKRYRFFSYGDASLLL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=343, Percent_Identity=54.5189504373178, Blast_Score=372, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39183; Mature: 39183

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNCTNLFLGCSMNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFREIAD
CCCCCEEEEECCCCCCCEEEECCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
FLQPGDLLVMNDSKVIPARLYGHKATGGKVELLVERITGDFTFLAHIKASKSLKSNDLIY
HHCCCCEEEECCCCCCCHHEECCCCCCCHHHHHEEHHCCCEEEEEEEHHCCCCCCCCEEE
LDAGKRLEVLQRQDDLFLCKACENILDLLNDLGHIPLPPYIAREDESLDKERYQTVYAKC
EECCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AGSVAAPTAGLHFDDAVLSSIRARGVNIAYVTLHVGAGTFRPVRCERIQDHKMHSEWFTV
CCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCCEEEE
SPDLCTAVKAAKSMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIFIYPGYQFKVCDG
CHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHCCCEEECCCCCCCEEEECCCEEEECCH
LITNFHLPESTLVMLVSAFIGHQECMALYQEAIDKRYRFFSYGDASLLL
HHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHEEEEECCCEECC
>Mature Secondary Structure
MYNCTNLFLGCSMNKQDFYFDLPSELIAQYPLANRSDSRLLIYNRQTEEYGHYQFREIAD
CCCCCEEEEECCCCCCCEEEECCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHH
FLQPGDLLVMNDSKVIPARLYGHKATGGKVELLVERITGDFTFLAHIKASKSLKSNDLIY
HHCCCCEEEECCCCCCCHHEECCCCCCCHHHHHEEHHCCCEEEEEEEHHCCCCCCCCEEE
LDAGKRLEVLQRQDDLFLCKACENILDLLNDLGHIPLPPYIAREDESLDKERYQTVYAKC
EECCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AGSVAAPTAGLHFDDAVLSSIRARGVNIAYVTLHVGAGTFRPVRCERIQDHKMHSEWFTV
CCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCCEEEE
SPDLCTAVKAAKSMGNRVIAVGTTALRSLESAAMGGELIPCSRDTDIFIYPGYQFKVCDG
CHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHCCCEEECCCCCCCEEEECCCEEEECCH
LITNFHLPESTLVMLVSAFIGHQECMALYQEAIDKRYRFFSYGDASLLL
HHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA