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The map label for this gene is fleR

Identifier: 52841989

GI number: 52841989

Start: 1966965

End: 1968326

Strand: Reverse

Name: fleR

Synonym: lpg1762

Alternate gene names: 52841989

Gene position: 1968326-1966965 (Counterclockwise)

Preceding gene: 52841990

Following gene: 52841988

Centisome position: 57.93

GC content: 39.21

Gene sequence:

>1362_bases
TTGCCTGAACAGAAACGTAGGGTAACTTTATCAAACAAAGGATTTTCTATGAGTCATGTTTTAATAGTTGAAGATGATTC
AGTATTAAGAGAAGCGTTGGCTGAGACTATGACGATCTCAGGCCATACCTATATCACTGCTAAAGACGGTAAAGAGGCAT
TAGCACTTCTTGAATTACATAATCCAAGTGTCGTACTCACTGACATAAGAATGGATAAATTAGATGGAAATCAGTTATTA
AATGAAATACAAAAAAAGAGGCCTGGTTTGCCGGTTATTCTAATGACAGCTTATGGTTCTGTAGAGGATGCGGTTACAGC
GATTCGCAATGGCGCTGTTGATTATTTGCAAAAGCCCTTCAGCGCGCAAGTCTTAATTGAAAAAATAAAGCAGTATATAA
AAACTGATCTGAGTGAAGAGGATAATGATCCTATTGCACGGGATCCTAAAAGCCAAGCCTTACTGACAATGGCTTTGAGA
GTCGCACAATCCGATGTGGGAGTAATGATCAGTGGAGAAAGCGGTACTGGAAAGGAAGTGCTCGCACATTTTATCCATGA
TCATTCTTATCGAAGCAAACAACCTTTTATTGCAATTAATTGTGCTGCAATTCCTGAACAAATGCTGGAAGCAACCCTGT
TTGGTTATGAAAAAGGTGCTTTTACCGGAGCATACAAATCAACCCCTGGCAAGTTTGAGCAAGCCCAGGGCGGAACTTTA
CTTTTAGATGAAGTAAGTGAAATGTCCTTAGGTTTGCAGGCAAAATTACTTAGAGTTTTACAGGAAAAAGAAGTAGAAAG
GATTGGTTCAAACAAAATAATAAACCTTGATGTGCGCATTATTGCGACGACAAACAGGCAATTAAAGGAAGAGGTTCAGG
CAGGGCGTTTTAGAGAGGACCTTTACTATCGATTGAATGTTTTTCCTCTGCAATGGCACCCACTAAGAGAGAGAACAAGT
GATATTATTCCATTAGCGAATTATTTAATCCGTAAACATTGTCAGCATAAACAACCAATAGTTCCAGTCATTAGCCCGGA
TGCGCAAAAGGCAATGTTGTCCTATTCATGGCCAGGGAATGCGAGAGAGCTGGATAATGTTATACAAAGAGCTCTGGTGT
TACAGACTCAAGGTATCATCGAAGAAGAGCATTTGCAGATTACGAATTCAACTACCATGCCTTCGGAAGAGAAGACAAAG
GGTACGAGTAAAAATTTACAGATACATGAGTATGAATTAATAGAAAAAACATTATTAGAAAATGAAGGAAACAGACAAAA
AGTTGCTGCGTTATTGGGGGTTAGCGAAAGGACTTTGCGCTATAAACTCGCAAAAATGCGTGAAGAAGGGTATGTGGTTT
AA

Upstream 100 bases:

>100_bases
TTGGATTAGCAGTGGTTTTTGCTGTAGTAAAAGCGCATGGTGGTCGAGTGACTCTTGAGTCGACTGAAGGGATAGGGACA
CAGGTAACTATTTATTTACC

Downstream 100 bases:

>100_bases
CAGGGGCTGAGAGAATGAGCGATATTAATACAGTAAATTTAATTAATCAAATGAAAACTCTGGCCGCCCAGGCTGAGGGG
ATAAAACCGGATTCTGGTTT

Product: sigma 54-dependent response regulator

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MPEQKRRVTLSNKGFSMSHVLIVEDDSVLREALAETMTISGHTYITAKDGKEALALLELHNPSVVLTDIRMDKLDGNQLL
NEIQKKRPGLPVILMTAYGSVEDAVTAIRNGAVDYLQKPFSAQVLIEKIKQYIKTDLSEEDNDPIARDPKSQALLTMALR
VAQSDVGVMISGESGTGKEVLAHFIHDHSYRSKQPFIAINCAAIPEQMLEATLFGYEKGAFTGAYKSTPGKFEQAQGGTL
LLDEVSEMSLGLQAKLLRVLQEKEVERIGSNKIINLDVRIIATTNRQLKEEVQAGRFREDLYYRLNVFPLQWHPLRERTS
DIIPLANYLIRKHCQHKQPIVPVISPDAQKAMLSYSWPGNARELDNVIQRALVLQTQGIIEEEHLQITNSTTMPSEEKTK
GTSKNLQIHEYELIEKTLLENEGNRQKVAALLGVSERTLRYKLAKMREEGYVV

Sequences:

>Translated_453_residues
MPEQKRRVTLSNKGFSMSHVLIVEDDSVLREALAETMTISGHTYITAKDGKEALALLELHNPSVVLTDIRMDKLDGNQLL
NEIQKKRPGLPVILMTAYGSVEDAVTAIRNGAVDYLQKPFSAQVLIEKIKQYIKTDLSEEDNDPIARDPKSQALLTMALR
VAQSDVGVMISGESGTGKEVLAHFIHDHSYRSKQPFIAINCAAIPEQMLEATLFGYEKGAFTGAYKSTPGKFEQAQGGTL
LLDEVSEMSLGLQAKLLRVLQEKEVERIGSNKIINLDVRIIATTNRQLKEEVQAGRFREDLYYRLNVFPLQWHPLRERTS
DIIPLANYLIRKHCQHKQPIVPVISPDAQKAMLSYSWPGNARELDNVIQRALVLQTQGIIEEEHLQITNSTTMPSEEKTK
GTSKNLQIHEYELIEKTLLENEGNRQKVAALLGVSERTLRYKLAKMREEGYVV
>Mature_452_residues
PEQKRRVTLSNKGFSMSHVLIVEDDSVLREALAETMTISGHTYITAKDGKEALALLELHNPSVVLTDIRMDKLDGNQLLN
EIQKKRPGLPVILMTAYGSVEDAVTAIRNGAVDYLQKPFSAQVLIEKIKQYIKTDLSEEDNDPIARDPKSQALLTMALRV
AQSDVGVMISGESGTGKEVLAHFIHDHSYRSKQPFIAINCAAIPEQMLEATLFGYEKGAFTGAYKSTPGKFEQAQGGTLL
LDEVSEMSLGLQAKLLRVLQEKEVERIGSNKIINLDVRIIATTNRQLKEEVQAGRFREDLYYRLNVFPLQWHPLRERTSD
IIPLANYLIRKHCQHKQPIVPVISPDAQKAMLSYSWPGNARELDNVIQRALVLQTQGIIEEEHLQITNSTTMPSEEKTKG
TSKNLQIHEYELIEKTLLENEGNRQKVAALLGVSERTLRYKLAKMREEGYVV

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=449, Percent_Identity=43.652561247216, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1788905, Length=441, Percent_Identity=42.8571428571429, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1790437, Length=446, Percent_Identity=40.5829596412556, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1790299, Length=461, Percent_Identity=38.3947939262473, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI87082117, Length=273, Percent_Identity=46.8864468864469, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI87082152, Length=298, Percent_Identity=44.9664429530201, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1789087, Length=321, Percent_Identity=43.3021806853583, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789233, Length=306, Percent_Identity=41.1764705882353, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1786524, Length=250, Percent_Identity=44.4, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI87081872, Length=301, Percent_Identity=38.5382059800665, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1787583, Length=312, Percent_Identity=36.2179487179487, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=34.4262295081967, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87081858, Length=312, Percent_Identity=31.4102564102564, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787375, Length=115, Percent_Identity=31.304347826087, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787487, Length=101, Percent_Identity=35.6435643564356, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87082012, Length=115, Percent_Identity=29.5652173913043, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=29.9145299145299, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790552, Length=115, Percent_Identity=31.304347826087, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50956; Mature: 50825

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEQKRRVTLSNKGFSMSHVLIVEDDSVLREALAETMTISGHTYITAKDGKEALALLELH
CCCCCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHEEECCCEEEEECCCCCEEEEEEEC
NPSVVLTDIRMDKLDGNQLLNEIQKKRPGLPVILMTAYGSVEDAVTAIRNGAVDYLQKPF
CCCEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCC
SAQVLIEKIKQYIKTDLSEEDNDPIARDPKSQALLTMALRVAQSDVGVMISGESGTGKEV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
LAHFIHDHSYRSKQPFIAINCAAIPEQMLEATLFGYEKGAFTGAYKSTPGKFEQAQGGTL
HHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCEE
LLDEVSEMSLGLQAKLLRVLQEKEVERIGSNKIINLDVRIIATTNRQLKEEVQAGRFRED
EEHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHCCHHHH
LYYRLNVFPLQWHPLRERTSDIIPLANYLIRKHCQHKQPIVPVISPDAQKAMLSYSWPGN
HEEEEEEEEECCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCHHHHEEEECCCCC
ARELDNVIQRALVLQTQGIIEEEHLQITNSTTMPSEEKTKGTSKNLQIHEYELIEKTLLE
HHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCHHCCCCCCCCEEHHHHHHHHHHHH
NEGNRQKVAALLGVSERTLRYKLAKMREEGYVV
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PEQKRRVTLSNKGFSMSHVLIVEDDSVLREALAETMTISGHTYITAKDGKEALALLELH
CCCCCEEEECCCCCCEEEEEEECCHHHHHHHHHHHEEECCCEEEEECCCCCEEEEEEEC
NPSVVLTDIRMDKLDGNQLLNEIQKKRPGLPVILMTAYGSVEDAVTAIRNGAVDYLQKPF
CCCEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCC
SAQVLIEKIKQYIKTDLSEEDNDPIARDPKSQALLTMALRVAQSDVGVMISGESGTGKEV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
LAHFIHDHSYRSKQPFIAINCAAIPEQMLEATLFGYEKGAFTGAYKSTPGKFEQAQGGTL
HHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCEE
LLDEVSEMSLGLQAKLLRVLQEKEVERIGSNKIINLDVRIIATTNRQLKEEVQAGRFRED
EEHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCHHHHHHHHHCCHHHH
LYYRLNVFPLQWHPLRERTSDIIPLANYLIRKHCQHKQPIVPVISPDAQKAMLSYSWPGN
HEEEEEEEEECCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCHHHHEEEECCCCC
ARELDNVIQRALVLQTQGIIEEEHLQITNSTTMPSEEKTKGTSKNLQIHEYELIEKTLLE
HHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCHHCCCCCCCCEEHHHHHHHHHHHH
NEGNRQKVAALLGVSERTLRYKLAKMREEGYVV
CCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]