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The map label for this gene is artJ

Identifier: 52841785

GI number: 52841785

Start: 1718670

End: 1719407

Strand: Reverse

Name: artJ

Synonym: lpg1555

Alternate gene names: 52841785

Gene position: 1719407-1718670 (Counterclockwise)

Preceding gene: 52841786

Following gene: 52841783

Centisome position: 50.6

GC content: 31.17

Gene sequence:

>738_bases
ATGAAGTTACTACAACTTCTCTTATTTATTATTATTTCCTCTAATTCTTATTCATTAAATTTAACAATTGGTACTTCAAA
ATTTAATCCGCCTTTTGAAGTTTGGTCTGGTAACAACAGTTCATTGTATGGCTTTGATATTGACCTTATGCAAGAAATAT
GTAGAAGATTACATGCGACCTGTACTTTTGAAGCCTATATATTCGATGATTTATTTCCCGCGTTAAAAAACCGCGAAGTC
GATCTGGTTATTGCCTCAATGATTATTACAGATGAAAGAAAGAAACACTTTATTTTTAGTCTTCCCTATATGGAAAGTAA
CTCACAATATATAACTACCGTCGATTCTAAAATCAGCACATTTGATGATTTGCACGGAAAAAAAATTGGAGTAAGAAAAG
GAACTCCTTACGCAAGGCAAGTTTTGTCAGAAAACCGCAACAATCAGGTAATTTTTTATGAGCTCATACAAGATATGCTT
CTTGGCTTAAGCAATAATCAAGTTGATGCCAGTTTAATGGATTATGAAGCAGCAAAATATTGGATGGCATCTGAACCCTA
TGCTTATAAATTAATTGGTAAGAAATATAAATTAATTGGTAAGAAAATTTCTATTGGAGAAGGGTATTCTATTATGGCAA
ACCCTGATCAATTTGTATTAATTAAAAAAATAAATAAAATTCTTCTTGAAATGGAAGCTGATGGAACTTATCTGAGACTA
TATTCAGAGTACTTTTAG

Upstream 100 bases:

>100_bases
AAACCAAATTAGAGAAATCATATGCTACAAATTGTACAAATCAAATTTTTTGTACCATGATTATGTTTAAACAAACTTCC
TAAATTACAGGATATAGTTT

Downstream 100 bases:

>100_bases
AAGAGGAGTATTAAGGACAAATAGCACCCAAAATACTCCAATTATACCTAAACAGGCTCCATTGTTTTTTTATTGGCCGA
GCTTTTTTGCTCCTTTTCTT

Product: arginine 3rd transport system periplasmic binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHATCTFEAYIFDDLFPALKNREV
DLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDML
LGLSNNQVDASLMDYEAAKYWMASEPYAYKLIGKKYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRL
YSEYF

Sequences:

>Translated_245_residues
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHATCTFEAYIFDDLFPALKNREV
DLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDML
LGLSNNQVDASLMDYEAAKYWMASEPYAYKLIGKKYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRL
YSEYF
>Mature_245_residues
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHATCTFEAYIFDDLFPALKNREV
DLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKISTFDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDML
LGLSNNQVDASLMDYEAAKYWMASEPYAYKLIGKKYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRL
YSEYF

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=243, Percent_Identity=32.5102880658436, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787088, Length=220, Percent_Identity=33.6363636363636, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788649, Length=254, Percent_Identity=31.8897637795276, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=29.957805907173, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1788228, Length=232, Percent_Identity=28.448275862069, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787031, Length=246, Percent_Identity=26.4227642276423, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28273; Mature: 28273

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHAT
CHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCEEEEECHHHHHHHHHHHCCE
CTFEAYIFDDLFPALKNREVDLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKIST
EEEEHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCEEEEECCEECCCCCEEEEECCHHHH
FDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDMLLGLSNNQVDASLMDYEAAKY
HHHCCCCEEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHH
WMASEPYAYKLIGKKYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRL
CCCCCCEEEEECCCCEEECCCEEECCCCEEEEECCCCEEHHHHHHHHEEEECCCCCEEEE
YSEYF
EECCC
>Mature Secondary Structure
MKLLQLLLFIIISSNSYSLNLTIGTSKFNPPFEVWSGNNSSLYGFDIDLMQEICRRLHAT
CHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECCCCCEEEEECHHHHHHHHHHHCCE
CTFEAYIFDDLFPALKNREVDLVIASMIITDERKKHFIFSLPYMESNSQYITTVDSKIST
EEEEHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCEEEEECCEECCCCCEEEEECCHHHH
FDDLHGKKIGVRKGTPYARQVLSENRNNQVIFYELIQDMLLGLSNNQVDASLMDYEAAKY
HHHCCCCEEECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHH
WMASEPYAYKLIGKKYKLIGKKISIGEGYSIMANPDQFVLIKKINKILLEMEADGTYLRL
CCCCCCEEEEECCCCEEECCCEEECCCCEEEEECCCCEEHHHHHHHHEEEECCCCCEEEE
YSEYF
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]