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The map label for this gene is pcnB

Identifier: 52841768

GI number: 52841768

Start: 1702965

End: 1704275

Strand: Direct

Name: pcnB

Synonym: lpg1538

Alternate gene names: 52841768

Gene position: 1702965-1704275 (Clockwise)

Preceding gene: 52841765

Following gene: 52841769

Centisome position: 50.12

GC content: 39.28

Gene sequence:

>1311_bases
CTGTTACGAAGAAACAGGTCGCATGGACATGCAATTGACTTGGCTTATATCATCCCGAGAAATAAACATAACATATCGAA
AGCAGATATAAGTAATAATGCGCTTAAAGTTCTTAACCGTTTAATCAGCCATGGTTTTCAGGCCTATATCGTTGGAGGCA
GTGTCAGAGATTTACTGCTCCATAAAGCGCCTAAAGATTTTGATATAGCGACAAATGCGACTCCCAATGAGGTAAAAAAT
CTTTTTAAAAATGCTCGTATCATTGGTCGTCGATTTAAACTGGTACATATTTTATTTCATCGTGAAATTATTGAAGTAGC
AACTTTTCGTGGCCATAACTCAAGCGATGCAAGCCACCAATTTAATGAACGCGGCATGCTTGTAAGAGATAATGTTTATG
GTTCTCTGGACGAAGACGCATGGAGACGTGATTTCACAATAAACTCTCTTTATTATAATATTTCTGATTCTTCAATCATT
GATTTTACTGGTGGTGTAGAAGACGTTAAGCAAAAGTTAATTCGAATAATTGGCGATCCCGTTACGCGTTATCAAGAGGA
TCCTGTCCGTATGTTACGTGCTGTCAGGTTTAGTGCCAAGCTGCAATTTAATTTATCTCCGGAAACCAAAGCTCCCTTCC
CCAAAATAAGCCACCTTATAACCCATGTTTCCAGTTCGCGACTATTTGATGAAATAACGAAACTTTATCAATGCGGAGAA
GCCGAATCGGTTCAAAAATTATTGGTCGAGTATGGTTTATTTAATTATCTATTTCCTCAAACAGCTGCTCTATTTGATAG
TCATTACCCGGTTAATCCGCTTTTGGGCATAGCACTTGAAAATACTGATACAAGAATACGTGATGGAAAGCCTGTGACTC
CGGCATTTCTTATTGCTGTTTTGTTATGGTTTCCAATGATAGAGTGTTCCAGAAAACTGCAAGAATCTGGGCTTCCGCCT
TTACCCTCGATTGAAAAAGCCATGTCTATAGTGCTTAGTGAACAGAATGCAATTATTTCGATACCTAAAAGGTATAGCCA
GGTTATAAGGGAAATTTGGCTTTTGCAATATCGCTTCGAAAAAAGATTTGGTGGCAGAGCATTTAATTTACTTCTTCATC
CTCGATTCAGAGCAGCGTATGATTTTATGGCTCTTAGGGCATTATCTGGTGATGAGTCAATGGAGCTGGCGCAGTGGTGG
GCCACGTTTCAAGAAGTCGATAATGTTGAAAAAAATAACATGGTAACCCAATTGGCTGCAACAACGCCCGGGCGGCCTAA
AAAACGTCGTAAACGGAAAATAGCAAAATGA

Upstream 100 bases:

>100_bases
TGTAAAGTAAATATTAAACTTGCAGGATAATTGAAAACACAACCGAATTACTGTTTAATCAATTTAGAGAGAAACAACAA
TCATCAAGTTTATTAAAAAA

Downstream 100 bases:

>100_bases
ATGTTTGCTATCTGAGCTTGGGATCGAATCAAAAAAATCCTGAGCGTCAAATAAGACAAGCCATTAATGAAATCAAACAG
ATTCCATCAACATGCCTTAT

Product: Poly(A) polymerase (PAP) (plasmid copy number protein)

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MLRRNRSHGHAIDLAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRDLLLHKAPKDFDIATNATPNEVKN
LFKNARIIGRRFKLVHILFHREIIEVATFRGHNSSDASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSII
DFTGGVEDVKQKLIRIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDEITKLYQCGE
AESVQKLLVEYGLFNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDGKPVTPAFLIAVLLWFPMIECSRKLQESGLPP
LPSIEKAMSIVLSEQNAIISIPKRYSQVIREIWLLQYRFEKRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWW
ATFQEVDNVEKNNMVTQLAATTPGRPKKRRKRKIAK

Sequences:

>Translated_436_residues
MLRRNRSHGHAIDLAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRDLLLHKAPKDFDIATNATPNEVKN
LFKNARIIGRRFKLVHILFHREIIEVATFRGHNSSDASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSII
DFTGGVEDVKQKLIRIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDEITKLYQCGE
AESVQKLLVEYGLFNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDGKPVTPAFLIAVLLWFPMIECSRKLQESGLPP
LPSIEKAMSIVLSEQNAIISIPKRYSQVIREIWLLQYRFEKRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWW
ATFQEVDNVEKNNMVTQLAATTPGRPKKRRKRKIAK
>Mature_436_residues
MLRRNRSHGHAIDLAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRDLLLHKAPKDFDIATNATPNEVKN
LFKNARIIGRRFKLVHILFHREIIEVATFRGHNSSDASHQFNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSII
DFTGGVEDVKQKLIRIIGDPVTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDEITKLYQCGE
AESVQKLLVEYGLFNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDGKPVTPAFLIAVLLWFPMIECSRKLQESGLPP
LPSIEKAMSIVLSEQNAIISIPKRYSQVIREIWLLQYRFEKRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWW
ATFQEVDNVEKNNMVTQLAATTPGRPKKRRKRKIAK

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=243, Percent_Identity=30.0411522633745, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI87081691, Length=411, Percent_Identity=49.6350364963504, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI1789436, Length=231, Percent_Identity=29.8701298701299, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=284, Percent_Identity=28.5211267605634, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321016, Length=213, Percent_Identity=27.6995305164319, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281360112, Length=211, Percent_Identity=28.9099526066351, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357337, Length=211, Percent_Identity=28.9099526066351, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 50048; Mature: 50048

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRNRSHGHAIDLAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRDLLL
CCCCCCCCCCEEEEEEEECCCCCCCCHHCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHH
HKAPKDFDIATNATPNEVKNLFKNARIIGRRFKLVHILFHREIIEVATFRGHNSSDASHQ
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
FNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSIIDFTGGVEDVKQKLIRIIGDP
HCCCCCEEECCCCCCCCHHHHHCCEEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCH
VTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDEITKLYQCGE
HHHCCCCHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
AESVQKLLVEYGLFNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDGKPVTPAFLIAV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHH
LLWFPMIECSRKLQESGLPPLPSIEKAMSIVLSEQNAIISIPKRYSQVIREIWLLQYRFE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHH
KRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWWATFQEVDNVEKNNMVTQLAA
HHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHC
TTPGRPKKRRKRKIAK
CCCCCHHHHHHHCCCC
>Mature Secondary Structure
MLRRNRSHGHAIDLAYIIPRNKHNISKADISNNALKVLNRLISHGFQAYIVGGSVRDLLL
CCCCCCCCCCEEEEEEEECCCCCCCCHHCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHH
HKAPKDFDIATNATPNEVKNLFKNARIIGRRFKLVHILFHREIIEVATFRGHNSSDASHQ
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
FNERGMLVRDNVYGSLDEDAWRRDFTINSLYYNISDSSIIDFTGGVEDVKQKLIRIIGDP
HCCCCCEEECCCCCCCCHHHHHCCEEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCH
VTRYQEDPVRMLRAVRFSAKLQFNLSPETKAPFPKISHLITHVSSSRLFDEITKLYQCGE
HHHCCCCHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
AESVQKLLVEYGLFNYLFPQTAALFDSHYPVNPLLGIALENTDTRIRDGKPVTPAFLIAV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHH
LLWFPMIECSRKLQESGLPPLPSIEKAMSIVLSEQNAIISIPKRYSQVIREIWLLQYRFE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHH
KRFGGRAFNLLLHPRFRAAYDFMALRALSGDESMELAQWWATFQEVDNVEKNNMVTQLAA
HHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHC
TTPGRPKKRRKRKIAK
CCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]