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The map label for this gene is prpD

Identifier: 52841759

GI number: 52841759

Start: 1692079

End: 1693551

Strand: Reverse

Name: prpD

Synonym: lpg1529

Alternate gene names: 52841759

Gene position: 1693551-1692079 (Counterclockwise)

Preceding gene: 52841760

Following gene: 52841757

Centisome position: 49.84

GC content: 40.94

Gene sequence:

>1473_bases
CTGCTATTGAAACACGAGGTTAATATGCACAATTATGTAGAGGATAATGTTAAGCCAGACTATGATCAGGTTATAACAGA
TATAGCTGATTTTGTATTAAATAAAGAAATTGATAGTGTGCAGGCCTATGAGACTGCGCGGTTATGCTTAATGGATACAC
TCGGTTGTGGCATATTGGCCCTTAACTTTCAGGAATGTACCAAATTAATGGGACCTATAGTACCGGGAGCAACTCTTCCT
GGAGGCGCGAGAGTACCAGGTACTAATTATGAACTGGATCCGGTTCAAGCTGCTTTTAATATCGGCACGATGATACGATG
GCTAGACTTTAATGATACTTGGCTCGCTGCAGAATGGGGGCACCCTTCTGATAATTTGGGTGCTATTTTGGCTGTTGCCG
ATTATTTGTGCCGGGAAAATTGTAATCATGGACGTCCGCCCATCTTGATGCATGATGTACTTACGGCAATGATTAAAGCA
CATGAAATTCAAGGTTGTTTGGCCCTGGAAAATAGCTTCAATCGCGTCGGTCTTGATCATGTCATATTAGTTAAAGTAGC
GAGTGCCGCTGTTGCTGCTCACTTGTTTGGAGCAGATCGTGATATGATGCTAAGAACTCTCTCGCAGGTTTTTGTGGATG
GACAAAGCTTGAGAACATATAGACATGCTCCCAATGCAGGTTCAAGGAAATCATGGGCAGCAGGAGATGCTACCTCCCGT
GCGGTTCGTTTGGCTTTAATTGCCAAGGCAGGGGAAATGGGATATCCAAGCGCCTTGACCGCACAAACATGGGGATTTTA
TGACGTGCTATTTGGTAAAAAACAATTCAAGTTTCAACGCCCTTACGGCAGCTATGTGATGGAAAATGTGTTATTCAAGT
TATCTTATCCTGCTGAATTTCATGCACAAACCGCTGTCGAATGTGCCGTAAAATTACATCCTATAGTAAAAAATAGATTA
GAGGATATTGCTAGAATTGAATTAATCACACATGAGTCAGCAATAAGAATAATAAGTAAGCAAGGTGCTTTATATAATCC
TGCCGACAGAGATCACTGCTTGCAATATATGGTTGCAGTAGGACTTCTCTTTGGTGATTTAAAGGCAGAGCATTATGAAG
ACGACGTTGCCGCAGATCCCAGAATAGATGCTTTACGAGCTAAAATGCATGTAACTGAAAATGAACGGTTTTCACGCGAT
TATCATGATCCGGAAAAACGCTCTATAGCTAACAGCATGAGAATCATCTTTAAAGATAGCTCAGAAAGTGAATTAATAAC
TATAGAATACCCTATTGGCCATAAACGGAGACGAGAAGAGGGGATTCCCGTTTTAATGTCTAAATTCAAGCGTAATCTGA
GTACAAGATTCTCAACTGACATGGTTGAAAAGATTATTTATGCCATGAGCGACACCAACACGCTATCATCTATGAAAGTA
AGTGATTTTATGGCCATGTGGATAGTAAATTAA

Upstream 100 bases:

>100_bases
CGCATCACAGGATGGTCTGCGCACGTATTTGAGCAAAGAGCAAATAATAAACTAATAAGACCTACTTCAGAATATATAGG
CCCAGATTCCAGGCCATTTC

Downstream 100 bases:

>100_bases
AAAGACTAACTAATACCCACGCTCAATCTGTTATCTGTACTGACTTTTAGCAAAGACCAGGAGGGGGCTAAGCGTATAGC
AAAGCCAACCCTCCTTACTA

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MLLKHEVNMHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMDTLGCGILALNFQECTKLMGPIVPGATLP
GGARVPGTNYELDPVQAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGAILAVADYLCRENCNHGRPPILMHDVLTAMIKA
HEIQGCLALENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNAGSRKSWAAGDATSR
AVRLALIAKAGEMGYPSALTAQTWGFYDVLFGKKQFKFQRPYGSYVMENVLFKLSYPAEFHAQTAVECAVKLHPIVKNRL
EDIARIELITHESAIRIISKQGALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENERFSRD
YHDPEKRSIANSMRIIFKDSSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFSTDMVEKIIYAMSDTNTLSSMKV
SDFMAMWIVN

Sequences:

>Translated_490_residues
MLLKHEVNMHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMDTLGCGILALNFQECTKLMGPIVPGATLP
GGARVPGTNYELDPVQAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGAILAVADYLCRENCNHGRPPILMHDVLTAMIKA
HEIQGCLALENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNAGSRKSWAAGDATSR
AVRLALIAKAGEMGYPSALTAQTWGFYDVLFGKKQFKFQRPYGSYVMENVLFKLSYPAEFHAQTAVECAVKLHPIVKNRL
EDIARIELITHESAIRIISKQGALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENERFSRD
YHDPEKRSIANSMRIIFKDSSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFSTDMVEKIIYAMSDTNTLSSMKV
SDFMAMWIVN
>Mature_490_residues
MLLKHEVNMHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMDTLGCGILALNFQECTKLMGPIVPGATLP
GGARVPGTNYELDPVQAAFNIGTMIRWLDFNDTWLAAEWGHPSDNLGAILAVADYLCRENCNHGRPPILMHDVLTAMIKA
HEIQGCLALENSFNRVGLDHVILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNAGSRKSWAAGDATSR
AVRLALIAKAGEMGYPSALTAQTWGFYDVLFGKKQFKFQRPYGSYVMENVLFKLSYPAEFHAQTAVECAVKLHPIVKNRL
EDIARIELITHESAIRIISKQGALYNPADRDHCLQYMVAVGLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENERFSRD
YHDPEKRSIANSMRIIFKDSSESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFSTDMVEKIIYAMSDTNTLSSMKV
SDFMAMWIVN

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=478, Percent_Identity=61.9246861924686, Blast_Score=635, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=486, Percent_Identity=56.3786008230453, Blast_Score=558, Evalue=1e-159,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 55068; Mature: 55068

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKHEVNMHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMDTLGCGILA
CCEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEE
LNFQECTKLMGPIVPGATLPGGARVPGTNYELDPVQAAFNIGTMIRWLDFNDTWLAAEWG
ECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCEEEEECC
HPSDNLGAILAVADYLCRENCNHGRPPILMHDVLTAMIKAHEIQGCLALENSFNRVGLDH
CCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCH
VILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNAGSRKSWAAGDATSR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCHHHH
AVRLALIAKAGEMGYPSALTAQTWGFYDVLFGKKQFKFQRPYGSYVMENVLFKLSYPAEF
HHEEHEEECCCCCCCCCCCCCCCCCHHHHHHCCHHHEEECCHHHHHHHHHHHEECCCHHH
HAQTAVECAVKLHPIVKNRLEDIARIELITHESAIRIISKQGALYNPADRDHCLQYMVAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
GLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENERFSRDYHDPEKRSIANSMRIIFKDS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHCEEEEEECC
SESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFSTDMVEKIIYAMSDTNTLSSMKV
CCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
SDFMAMWIVN
HHHHEEEECC
>Mature Secondary Structure
MLLKHEVNMHNYVEDNVKPDYDQVITDIADFVLNKEIDSVQAYETARLCLMDTLGCGILA
CCEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEE
LNFQECTKLMGPIVPGATLPGGARVPGTNYELDPVQAAFNIGTMIRWLDFNDTWLAAEWG
ECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCEEEEECC
HPSDNLGAILAVADYLCRENCNHGRPPILMHDVLTAMIKAHEIQGCLALENSFNRVGLDH
CCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCH
VILVKVASAAVAAHLFGADRDMMLRTLSQVFVDGQSLRTYRHAPNAGSRKSWAAGDATSR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCHHHH
AVRLALIAKAGEMGYPSALTAQTWGFYDVLFGKKQFKFQRPYGSYVMENVLFKLSYPAEF
HHEEHEEECCCCCCCCCCCCCCCCCHHHHHHCCHHHEEECCHHHHHHHHHHHEECCCHHH
HAQTAVECAVKLHPIVKNRLEDIARIELITHESAIRIISKQGALYNPADRDHCLQYMVAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
GLLFGDLKAEHYEDDVAADPRIDALRAKMHVTENERFSRDYHDPEKRSIANSMRIIFKDS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHCEEEEEECC
SESELITIEYPIGHKRRREEGIPVLMSKFKRNLSTRFSTDMVEKIIYAMSDTNTLSSMKV
CCCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
SDFMAMWIVN
HHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]