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The map label for this gene is dusA [H]

Identifier: 52841692

GI number: 52841692

Start: 1613697

End: 1614701

Strand: Direct

Name: dusA [H]

Synonym: lpg1462

Alternate gene names: 52841692

Gene position: 1613697-1614701 (Clockwise)

Preceding gene: 52841691

Following gene: 161723254

Centisome position: 47.49

GC content: 35.92

Gene sequence:

>1005_bases
TTGATATTAAACGTGGTTAATAGTATTTTTTCTAAATTATCTATAGCGCCGATGATTGATTGGACTAACAGTCCATTTCG
TACTCTGATGAGGTTATTGGCTCCTAACGCCCTTTTGTATACTGAAATGCAAACAACAGGGGCTGTGCAAAATAATCCTG
ACAGAGCTTTGTATTTTAATTCTGTTGAACATCCTCTCGCTATTCAATTGGGTGGGGCAGATAAATCCGCATTAGTTAAG
TGCGCCATTATTGCTGAACAAAAAGGGTTTAATGAAATTAATTTGAATTTGGGATGTCCTAGTGACAAAGTACAGGCGGG
ACGATTTGGGGCCTGCTTGATGAAGGAGCCAGAACACGTTGCTGATTGTATTCGTGCAATAAAACAGGTAGTAGGAATAC
CAGTCACAGCGAAAACGAGGATCGGTATTGATAACCAGGACAGTTATGAGTTTTTCAGATCTTTTTCTCTTCACTTGGTT
GAAGCAGGTTGTGATAAGCTTATTATTCATGCTCGTAAGGCTTGGTTAAATGGGTTAAATCCAAAGCAAAATAGAACGAT
TCCTCCCGTAAATTATGATTATGTATATGAACTAAAAAAAGAAATTCCCCATATCTTTATTACAATCAATGGGAATATAT
TAACTGTTGAAGAAATAAACAACCATTTAAAGCATGTCGATGGAGTAATGCTAGGACGGCTTGCTTGCGATAACCCCTAT
CAAATTGCTGAAATTCATCAAGCCTTATACCCAGAATTCATCAAAATTAAACGTAGCCAGTTGTTAATGAATTATCTAGA
TTATTGGTTTGATCAATCTCGAGCCAATCTCTCGTTAAGCATTTTGGTTAAGCCACTTTTTAATTTAGCTTTTGGATTAC
CGGGAGCCAAACATTGGAAAAAGAAATTGATGAATATTATTCAGGAGAAAAATACTTCCGGTTTTAGTGAGTTAATCCAG
TATTTATTAAATTTGGAAGCAATAAATCAAGTCGAGTTTGTATAA

Upstream 100 bases:

>100_bases
TCCAGGCAATGAATCCTGTTCAGCAAGCATAAACTCTTAAATTGATGCTATTTTTGTGTATTTCTCAAACAACAATGTAT
AATGTTATATTTGCTTTAAA

Downstream 100 bases:

>100_bases
GAAATAATAGAGCAGATGTCAACATTTCGTGGAATTTTATCAAGATTTCTAGTACATTAACGAGTTTATTAATTGGAACA
TCCTGAGGGTTATTGAGGCC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MILNVVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGAVQNNPDRALYFNSVEHPLAIQLGGADKSALVK
CAIIAEQKGFNEINLNLGCPSDKVQAGRFGACLMKEPEHVADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLV
EAGCDKLIIHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINNHLKHVDGVMLGRLACDNPY
QIAEIHQALYPEFIKIKRSQLLMNYLDYWFDQSRANLSLSILVKPLFNLAFGLPGAKHWKKKLMNIIQEKNTSGFSELIQ
YLLNLEAINQVEFV

Sequences:

>Translated_334_residues
MILNVVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGAVQNNPDRALYFNSVEHPLAIQLGGADKSALVK
CAIIAEQKGFNEINLNLGCPSDKVQAGRFGACLMKEPEHVADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLV
EAGCDKLIIHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINNHLKHVDGVMLGRLACDNPY
QIAEIHQALYPEFIKIKRSQLLMNYLDYWFDQSRANLSLSILVKPLFNLAFGLPGAKHWKKKLMNIIQEKNTSGFSELIQ
YLLNLEAINQVEFV
>Mature_334_residues
MILNVVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGAVQNNPDRALYFNSVEHPLAIQLGGADKSALVK
CAIIAEQKGFNEINLNLGCPSDKVQAGRFGACLMKEPEHVADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLV
EAGCDKLIIHARKAWLNGLNPKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINNHLKHVDGVMLGRLACDNPY
QIAEIHQALYPEFIKIKRSQLLMNYLDYWFDQSRANLSLSILVKPLFNLAFGLPGAKHWKKKLMNIIQEKNTSGFSELIQ
YLLNLEAINQVEFV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=265, Percent_Identity=23.3962264150943, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI239788462, Length=268, Percent_Identity=23.134328358209, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=28.3898305084746, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI145693211, Length=298, Percent_Identity=46.6442953020134, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1789660, Length=245, Percent_Identity=26.1224489795918, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=258, Percent_Identity=27.1317829457364, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24580595, Length=234, Percent_Identity=28.2051282051282, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19920448, Length=234, Percent_Identity=28.2051282051282, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37796; Mature: 37796

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNVVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGAVQNNPDRALYFN
CHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCEEEEC
SVEHPLAIQLGGADKSALVKCAIIAEQKGFNEINLNLGCPSDKVQAGRFGACLMKEPEHV
CCCCCEEEEECCCCHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHCCCCHHH
ADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGCDKLIIHARKAWLNGLN
HHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
PKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINNHLKHVDGVMLGRLACDNPY
CCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCCCH
QIAEIHQALYPEFIKIKRSQLLMNYLDYWFDQSRANLSLSILVKPLFNLAFGLPGAKHWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCHHHHH
KKLMNIIQEKNTSGFSELIQYLLNLEAINQVEFV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILNVVNSIFSKLSIAPMIDWTNSPFRTLMRLLAPNALLYTEMQTTGAVQNNPDRALYFN
CHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCEEEEC
SVEHPLAIQLGGADKSALVKCAIIAEQKGFNEINLNLGCPSDKVQAGRFGACLMKEPEHV
CCCCCEEEEECCCCHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHCCCCHHH
ADCIRAIKQVVGIPVTAKTRIGIDNQDSYEFFRSFSLHLVEAGCDKLIIHARKAWLNGLN
HHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
PKQNRTIPPVNYDYVYELKKEIPHIFITINGNILTVEEINNHLKHVDGVMLGRLACDNPY
CCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCCCH
QIAEIHQALYPEFIKIKRSQLLMNYLDYWFDQSRANLSLSILVKPLFNLAFGLPGAKHWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCHHHHH
KKLMNIIQEKNTSGFSELIQYLLNLEAINQVEFV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]