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The map label for this gene is rumA

Identifier: 52841686

GI number: 52841686

Start: 1605883

End: 1607217

Strand: Direct

Name: rumA

Synonym: lpg1456

Alternate gene names: 52841686

Gene position: 1605883-1607217 (Clockwise)

Preceding gene: 52841683

Following gene: 52841687

Centisome position: 47.26

GC content: 37.75

Gene sequence:

>1335_bases
ATGAGAAAAGTTAAGCCAAAATTGAATTTAACCTCACAAACCGCTCGGATCGTAAATTTAAGCCATGATGGAAGGGGGAT
AGCACGAGTTAATGGCAAAGCAACCTTTATTCAGGGAGCATTACCCGGTGAGATGGTTGAGTTTCAGTATACACGGGTAA
AAAAGGATTTTGATGAAGGTAAATTATTATCAATTATCGAGCCCTCTACTCTCAGGGTTGAACCAAAATGCCCTCATTAT
CAAATGTGTGGAGGCTGTTCTTTGCAACATATGAGTGCCGAGGAACAAATTCGTTTCAAACAATCCCATCTTTTGGATTT
GCTATCCAGGTATGGCCATACTGAACCTCAATCAATGTTGTCGCCTTTAACAAGCCATCATTGGAATTATAGAAATAAAG
CACGCTTAAGTACTCGCTTTGTAGAAAAGAAACAAAGCACCATGGTTGGGTTTAGAGAGAGAAATAATCCAAGGTTCATT
ACAGAAATAAATCAATGTCCTATTCTTAATTCAAAAATAGATACTGATATTGTTCATTTAAGAAAGTTAATTGATACGAT
GGAGGATAAGCAGTGTATCGCACAAATTGAAGTGGCTGCAGGTGATAATGAAGTGGCGCTGATTTTCCGCAATCTAACCC
CATTAACAGAGCAAGATGAGTTGAAAATCAGGAAATTTGCGGAACAATTCCAATATAAGGTATTCCTTCAGCCAGGTGGA
CTTGATTCGGTTTTTTGTTTTTATCCTTCAGATGCCCATGCTTATTTGAGTTATGAGCTGCCCGATTATCAAATAACTTT
TCAATTTCATCCCAACGATTTTACTCAGGTAAACGCCGAGTTAAACAGAAAGATGGTAACCCAGGCAATTCAGTTAATGG
AATTAAAAAATTCTGATATCGTGCTAGACTTATTTTGTGGACTGGGTAATTTTTCGCTTCCCATGGCTAAACATTGCTCT
CGAGTTATCGGTGTCGAAGGCAATAAGAACATGGTTGAAAGAGCCTATATGAATGCCAAGTCTAATCATATTACCAATGT
AGATTTTTATGCTGCTAATCTTGATGATGTGATGGAAGTTAGGAATCTGGTTAATACGTCTTTTAGCAAGGTTTTAATCG
ATCCTCCCCGGTCTGGTGCGCTTGAAATTGTAAAACAAATTGATTCAATTGATCCCGAGCGTATAGTATATGTATCTTGT
AACCCGATAACATTAGCTCGAGATACAGATATATTGGTTAATCAAAAAGGTTACGTTTTGATAACAGCTGGGGTAATGGA
TATGTTTCCTCATACAGCGCATGTCGAGTCTATTGCCTTGTTTCAAAAAGGATAA

Upstream 100 bases:

>100_bases
TGGCCCTTTATTTGGGCATTTTTTGTTTTTTATATCCATACAACATTCTAAAAATGTAGCATGAGAATTTTGTAATTGAT
AATTATTTGAGAAACATTTC

Downstream 100 bases:

>100_bases
TATGTATGGTAAGAGTAAAAGATACGACTCCGTTGACGCCAGATGGCAGTATTGACGTAGAGATGTGGTTACATCATCTT
GGTTCAAAAGGATATTTGGA

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGEMVEFQYTRVKKDFDEGKLLSIIEPSTLRVEPKCPHY
QMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTEPQSMLSPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
TEINQCPILNSKIDTDIVHLRKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQDELKIRKFAEQFQYKVFLQPGG
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVTQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCS
RVIGVEGNKNMVERAYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSC
NPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG

Sequences:

>Translated_444_residues
MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGEMVEFQYTRVKKDFDEGKLLSIIEPSTLRVEPKCPHY
QMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTEPQSMLSPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
TEINQCPILNSKIDTDIVHLRKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQDELKIRKFAEQFQYKVFLQPGG
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVTQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCS
RVIGVEGNKNMVERAYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSC
NPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG
>Mature_444_residues
MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGEMVEFQYTRVKKDFDEGKLLSIIEPSTLRVEPKCPHY
QMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTEPQSMLSPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFI
TEINQCPILNSKIDTDIVHLRKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQDELKIRKFAEQFQYKVFLQPGG
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVTQAIQLMELKNSDIVLDLFCGLGNFSLPMAKHCS
RVIGVEGNKNMVERAYMNAKSNHITNVDFYAANLDDVMEVRNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSC
NPITLARDTDILVNQKGYVLITAGVMDMFPHTAHVESIALFQKG

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=193, Percent_Identity=32.1243523316062, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI51173878, Length=193, Percent_Identity=32.1243523316062, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI269784680, Length=205, Percent_Identity=27.8048780487805, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI269784676, Length=205, Percent_Identity=27.8048780487805, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI20149688, Length=205, Percent_Identity=27.8048780487805, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI269784678, Length=205, Percent_Identity=27.8048780487805, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789148, Length=433, Percent_Identity=35.796766743649, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1790403, Length=177, Percent_Identity=32.7683615819209, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787083, Length=180, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24666579, Length=435, Percent_Identity=27.1264367816092, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 50567; Mature: 50567

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGEMVEFQYTRVKKDFDEG
CCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCEEEEECCCCCCHHHEEHHHHHCCCCCC
KLLSIIEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTEPQSML
CEEEEECCCEEEECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFITEINQCPILNSKIDTDIVHL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECCCCCCCCCCCCHHHHHH
RKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQDELKIRKFAEQFQYKVFLQPGG
HHHHHHHHCHHEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCC
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVTQAIQLMELKNSDI
CCCEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEECHHHHHHHHHHHHHHHHCCCCCE
VLDLFCGLGNFSLPMAKHCSRVIGVEGNKNMVERAYMNAKSNHITNVDFYAANLDDVMEV
EEEEEECCCCCCCCHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
RNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSCNPITLARDTDILVNQKGYVL
HHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEECCCEEE
ITAGVMDMFPHTAHVESIALFQKG
EEECHHHHCCCHHHHHEEEEEECC
>Mature Secondary Structure
MRKVKPKLNLTSQTARIVNLSHDGRGIARVNGKATFIQGALPGEMVEFQYTRVKKDFDEG
CCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCEEEEECCCCCCHHHEEHHHHHCCCCCC
KLLSIIEPSTLRVEPKCPHYQMCGGCSLQHMSAEEQIRFKQSHLLDLLSRYGHTEPQSML
CEEEEECCCEEEECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SPLTSHHWNYRNKARLSTRFVEKKQSTMVGFRERNNPRFITEINQCPILNSKIDTDIVHL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCCEEEEECCCCCCCCCCCCHHHHHH
RKLIDTMEDKQCIAQIEVAAGDNEVALIFRNLTPLTEQDELKIRKFAEQFQYKVFLQPGG
HHHHHHHHCHHEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCC
LDSVFCFYPSDAHAYLSYELPDYQITFQFHPNDFTQVNAELNRKMVTQAIQLMELKNSDI
CCCEEEECCCCCCEEEEEECCCEEEEEEECCCCCEEECHHHHHHHHHHHHHHHHCCCCCE
VLDLFCGLGNFSLPMAKHCSRVIGVEGNKNMVERAYMNAKSNHITNVDFYAANLDDVMEV
EEEEEECCCCCCCCHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
RNLVNTSFSKVLIDPPRSGALEIVKQIDSIDPERIVYVSCNPITLARDTDILVNQKGYVL
HHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEECCCCEEEECCCEEE
ITAGVMDMFPHTAHVESIALFQKG
EEECHHHHCCCHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA