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The map label for this gene is hsrA [H]

Identifier: 52841684

GI number: 52841684

Start: 1603092

End: 1604477

Strand: Reverse

Name: hsrA [H]

Synonym: lpg1454

Alternate gene names: 52841684

Gene position: 1604477-1603092 (Counterclockwise)

Preceding gene: 52841685

Following gene: 52841681

Centisome position: 47.22

GC content: 37.16

Gene sequence:

>1386_bases
ATGAAGAAAAATATTATTATGTTCATTGTATCATGCGCCATGTTTATGGAGGCTATTGATACAACTATTATCAACACCGC
AATCCCGGTAATGGCTCATAGTTTAGAGGTCAATCCAATTGATCTCAAACTTGCGCTCATTAGCTATCTTTTAAGCTTGG
CAATCTTTATTCCTATAAGTGGATGGATTGCTGATAAATATGGTGTAAAAAAAGTATTTATAGCAGCTATTGGTGTCTTT
ACTCTAAGTTCCTTATGGTGCGGATTCACTCAAAGCCTTTGGGAATTAGTATTAGCCAGAATTATACAGGGTGTGGGCGG
CTCCCTAACCGTGCCTGTTGGACGTTTAATTATTCTGAGAACTTGTGAGCGTCACGAATTAATAACAAAAATGAGTATTG
TCGTCATGATTGCCTCGCTAGGTATGCTACTTGGTCCCTTACTAGGCGGTATTATTACTGATTATTTCTCATGGCGGTGG
ATATTTTGGGTTAATATCCCTGTTGGCATAATCGCCATGCTTTTATCTCACAAACTGCTTCCAACTATCCTGCCCAGATC
GGTTCCACCTTTGGATAAATCAGGATTTATAATGTTCGGAGCAGGCCTTGCATTCTTGACTTTAGGCTTGTCTTTGTTTA
GTGAGACGAAAGCGGATATCGTTTACTCCGCTTCAATTACTCTCTTATCCGCCTTGCTGTTAATTGGATATACCAAACAT
TCTCGTAGCAAAAGGAATCCTATTATAAAAGTTACTTTGTTAAATATAAGAACATTTCGTATTGCCGTGTTTGGAAATTT
ATTAACTCGTTTAAGTTTTGGTGGAATTCCCTTTTTATTACCTTTGCTCTTCCAAATTATTTTAGAATATTCTCCCCAGT
TGTCAGGAATGTTGCTTACACCAGTGGCATTAGGCGTATTCCTGGTTAAGCCATTATCTTTCTCCATCTTACGAAGATTG
GGTTATAAAAAATTACTCCTTTTAAATACCGTTTTAGTCTGTTTTTCTCTCTGGTCCTTTGCCACAATAAATCAAAGCAC
TTCTGTCATCTACATTGGTTTTTTAACATTTCTTTATGGATTTTTCATTGCTCTGCAATACACAGGGATGAATTCCCTTG
CGTATGCGAATATTGACGATAATGATATGAGTTATGCGACAAGTATTATGAGCACAGTTCAACAGTTATCCCAAAGTTTT
GGAGTAGCAATTTCTGCCCTACTGGTCAGTTTATTCACTTTTCAGGTTTCGCAGCATTTTGTTTTAACAGTCAAGATTTT
TCATCTAACCTTTTTTGCTTTAGGGATACTTACGATTCTTTCAGGATTGATATTTACCAGCTTAAAAAAAGAGGATGGAA
AAGAACTGATTGAGTCACCCACCTAG

Upstream 100 bases:

>100_bases
TATGATCATCATTGGACAAAATACTGGAGCGGGCTTGTGACTTTAATAGAGTTTCAGGTTAATAAAATTTAAACGTTCTA
ACTTCCATAAAACAGGCATT

Downstream 100 bases:

>100_bases
GTCCATCTCAGTTGTATCTTGTAAGCCAGACACCTTGATGACCAAGACAATGCATGTCAATACAGTATCTGGTCATTCAA
GAAAAAATTAATGCTTAAGG

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKNIIMFIVSCAMFMEAIDTTIINTAIPVMAHSLEVNPIDLKLALISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVF
TLSSLWCGFTQSLWELVLARIIQGVGGSLTVPVGRLIILRTCERHELITKMSIVVMIASLGMLLGPLLGGIITDYFSWRW
IFWVNIPVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKADIVYSASITLLSALLLIGYTKH
SRSKRNPIIKVTLLNIRTFRIAVFGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGMLLTPVALGVFLVKPLSFSILRRL
GYKKLLLLNTVLVCFSLWSFATINQSTSVIYIGFLTFLYGFFIALQYTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSF
GVAISALLVSLFTFQVSQHFVLTVKIFHLTFFALGILTILSGLIFTSLKKEDGKELIESPT

Sequences:

>Translated_461_residues
MKKNIIMFIVSCAMFMEAIDTTIINTAIPVMAHSLEVNPIDLKLALISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVF
TLSSLWCGFTQSLWELVLARIIQGVGGSLTVPVGRLIILRTCERHELITKMSIVVMIASLGMLLGPLLGGIITDYFSWRW
IFWVNIPVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKADIVYSASITLLSALLLIGYTKH
SRSKRNPIIKVTLLNIRTFRIAVFGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGMLLTPVALGVFLVKPLSFSILRRL
GYKKLLLLNTVLVCFSLWSFATINQSTSVIYIGFLTFLYGFFIALQYTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSF
GVAISALLVSLFTFQVSQHFVLTVKIFHLTFFALGILTILSGLIFTSLKKEDGKELIESPT
>Mature_461_residues
MKKNIIMFIVSCAMFMEAIDTTIINTAIPVMAHSLEVNPIDLKLALISYLLSLAIFIPISGWIADKYGVKKVFIAAIGVF
TLSSLWCGFTQSLWELVLARIIQGVGGSLTVPVGRLIILRTCERHELITKMSIVVMIASLGMLLGPLLGGIITDYFSWRW
IFWVNIPVGIIAMLLSHKLLPTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKADIVYSASITLLSALLLIGYTKH
SRSKRNPIIKVTLLNIRTFRIAVFGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGMLLTPVALGVFLVKPLSFSILRRL
GYKKLLLLNTVLVCFSLWSFATINQSTSVIYIGFLTFLYGFFIALQYTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSF
GVAISALLVSLFTFQVSQHFVLTVKIFHLTFFALGILTILSGLIFTSLKKEDGKELIESPT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=451, Percent_Identity=37.4722838137472, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1788392, Length=453, Percent_Identity=35.0993377483444, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1789042, Length=455, Percent_Identity=24.1758241758242, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1788710, Length=411, Percent_Identity=27.007299270073, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87081983, Length=398, Percent_Identity=26.8844221105528, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790146, Length=180, Percent_Identity=25.5555555555556, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=131, Percent_Identity=33.587786259542, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=128, Percent_Identity=36.71875, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=172, Percent_Identity=31.3953488372093, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=188, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50926; Mature: 50926

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNIIMFIVSCAMFMEAIDTTIINTAIPVMAHSLEVNPIDLKLALISYLLSLAIFIPIS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
GWIADKYGVKKVFIAAIGVFTLSSLWCGFTQSLWELVLARIIQGVGGSLTVPVGRLIILR
CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHH
TCERHELITKMSIVVMIASLGMLLGPLLGGIITDYFSWRWIFWVNIPVGIIAMLLSHKLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHH
PTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKADIVYSASITLLSALLLIGYTKH
HHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
SRSKRNPIIKVTLLNIRTFRIAVFGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGMLLT
CCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHH
PVALGVFLVKPLSFSILRRLGYKKLLLLNTVLVCFSLWSFATINQSTSVIYIGFLTFLYG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
FFIALQYTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSFGVAISALLVSLFTFQVSQHF
HHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VLTVKIFHLTFFALGILTILSGLIFTSLKKEDGKELIESPT
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MKKNIIMFIVSCAMFMEAIDTTIINTAIPVMAHSLEVNPIDLKLALISYLLSLAIFIPIS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
GWIADKYGVKKVFIAAIGVFTLSSLWCGFTQSLWELVLARIIQGVGGSLTVPVGRLIILR
CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHH
TCERHELITKMSIVVMIASLGMLLGPLLGGIITDYFSWRWIFWVNIPVGIIAMLLSHKLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHH
PTILPRSVPPLDKSGFIMFGAGLAFLTLGLSLFSETKADIVYSASITLLSALLLIGYTKH
HHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
SRSKRNPIIKVTLLNIRTFRIAVFGNLLTRLSFGGIPFLLPLLFQIILEYSPQLSGMLLT
CCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHH
PVALGVFLVKPLSFSILRRLGYKKLLLLNTVLVCFSLWSFATINQSTSVIYIGFLTFLYG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
FFIALQYTGMNSLAYANIDDNDMSYATSIMSTVQQLSQSFGVAISALLVSLFTFQVSQHF
HHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VLTVKIFHLTFFALGILTILSGLIFTSLKKEDGKELIESPT
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]