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The map label for this gene is pyrF

Identifier: 52841655

GI number: 52841655

Start: 1574217

End: 1574906

Strand: Direct

Name: pyrF

Synonym: lpg1425

Alternate gene names: 52841655

Gene position: 1574217-1574906 (Clockwise)

Preceding gene: 52841654

Following gene: 52841656

Centisome position: 46.33

GC content: 40.43

Gene sequence:

>690_bases
ATGACACCTAAATTAATCGTTGCGTTGGATTTTGATAATCAAGATAACGCTATACAGTTGGTTGAAAAACTTGATCCGAA
TCATTGCGCACTTAAGGTTGGGAGTGAGCTTTTTACTCTATTAGGGCCTCAGTTTGTAAAAGCATTGGTAAGAAGAGAGT
TTAAAGTATTTTTAGATTTAAAATTTCACGATATCCCTAATACAGTTGCTAAAGCATGCCACTCCGCTGCAGAATTAGGG
GTATGGATGATAAATGTTCATGCCATCGGCGGCTTAAAAATGTTACAAGCTGCCAGGGAATCTTTAAAAACGTATGGTAA
AGACAGGCCTTTGTTGATAGCAGTGACTGTGCTGACCAGTTTTGAAGAAGGGGAGCTGGCCAGTGTTGGTATTAGTAACA
CATTGCCTGAGCAAGCAACCCATCTGGCTATGCTTGCCAGAGAGGCTGGGCTTGATGGTGTGGTAAGCTCTGCACATGAA
GTTAAAATTATAAAACAGAAGTGTGGTGAGAATTTTATTACCGTTACTCCTGGCATAAGACTGCCAAATAATCTAAAAGA
TGATCAATCGCGAGTAATGACTCCACAACAAGCCATCAGAGAAGGAAGTGATTTTCTGGTTATTGGAAGACCAATTACGC
AAGCGAGCAATCCTTATGAGGTTGTCTCAGCATTATTACGTGATCTTTGA

Upstream 100 bases:

>100_bases
CCTGCCTATGCACCCTTATTTACAAGAAGATGAGCAATTGAAAATAGTTGCAGCAGTTAGAAATGCTTTACTTGTGATGA
ATGAAGAGGCCATGGTATAA

Downstream 100 bases:

>100_bases
TGGCGCATACTTTTAAAAAATTATACATATGGTATAATTTTTTTCATTTTTGAATTAGTTGTACCATTCCTATTTTATTC
CATAGAGATTTATGATAATT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTPKLIVALDFDNQDNAIQLVEKLDPNHCALKVGSELFTLLGPQFVKALVRREFKVFLDLKFHDIPNTVAKACHSAAELG
VWMINVHAIGGLKMLQAARESLKTYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHE
VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPYEVVSALLRDL

Sequences:

>Translated_229_residues
MTPKLIVALDFDNQDNAIQLVEKLDPNHCALKVGSELFTLLGPQFVKALVRREFKVFLDLKFHDIPNTVAKACHSAAELG
VWMINVHAIGGLKMLQAARESLKTYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHE
VKIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPYEVVSALLRDL
>Mature_228_residues
TPKLIVALDFDNQDNAIQLVEKLDPNHCALKVGSELFTLLGPQFVKALVRREFKVFLDLKFHDIPNTVAKACHSAAELGV
WMINVHAIGGLKMLQAARESLKTYGKDRPLLIAVTVLTSFEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHEV
KIIKQKCGENFITVTPGIRLPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPYEVVSALLRDL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=221, Percent_Identity=54.2986425339367, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25077; Mature: 24946

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPKLIVALDFDNQDNAIQLVEKLDPNHCALKVGSELFTLLGPQFVKALVRREFKVFLDL
CCCCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHEEEEEE
KFHDIPNTVAKACHSAAELGVWMINVHAIGGLKMLQAARESLKTYGKDRPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHC
FEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHEVKIIKQKCGENFITVTPGIR
CCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
LPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPYEVVSALLRDL
CCCCCCCHHHHCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TPKLIVALDFDNQDNAIQLVEKLDPNHCALKVGSELFTLLGPQFVKALVRREFKVFLDL
CCCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHEEEEEE
KFHDIPNTVAKACHSAAELGVWMINVHAIGGLKMLQAARESLKTYGKDRPLLIAVTVLTS
EECCCCHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEEHHC
FEEGELASVGISNTLPEQATHLAMLAREAGLDGVVSSAHEVKIIKQKCGENFITVTPGIR
CCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
LPNNLKDDQSRVMTPQQAIREGSDFLVIGRPITQASNPYEVVSALLRDL
CCCCCCCHHHHCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA