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The map label for this gene is gspF

Identifier: 52841593

GI number: 52841593

Start: 1507923

End: 1509122

Strand: Reverse

Name: gspF

Synonym: lpg1363

Alternate gene names: 52841593

Gene position: 1509122-1507923 (Counterclockwise)

Preceding gene: 52841600

Following gene: 52841592

Centisome position: 44.42

GC content: 40.17

Gene sequence:

>1200_bases
ATGGGCGCCTACCAATATCAAGCGCTAAAAGTGAATGGCAGCATTAGCAAAGGTGTAATAGAAGCTGATTCTGAACGCCA
TGCACGTCAATTATTGCGAGAACAAGGACTAATCCCTACTCAAGTCCAAACATTAACCCAGCAACGTGCTGCCAGCAGCA
AAAGTAAAGTTTCTGCGGCTGATTTGGCATTACTCACGAGACAGCTGGCAACACTTCTGGCTGCCGGTATTCCTGTTGAA
GAATCATTGCGCGGGGTCAGCGAACAAACAGAAAAAGACAAAGTCAGAGAGCTTATTATCGGAGTTCGCTCTAAAGTATT
GGAAGGGTATGGTCTTGCTCAGGCAATGGCTCAATATCCCAATGCCTTTCCAGAACTATACCGGGCAACAGTTGGCTCAG
GCGAGCAGACAGGGCGTCTTGATGTCGTTTTAGAAAAATTAGCTGATTATACTGAAAAACAACAACAAACTCGACAGAAA
GTGCAACAAGCCTTGATTTACCCATTGCTCATGATCATTGTTTCTACTGCCATTATCAGTTTTTTACTTACTTTTGTCGT
CCCTAAAATTATTGATGTGTTTACCGAAAGTGGCCAAACACTACCACCAATGACGAAATTGCTTATTAACTTGAGCCAAT
TTATCAAATCTTATGGTTTGTATAGTCTAGTGACAATAATTGTGGCTCTGATTGGATTTAAAAAAAGCCTCAGTAATATA
AAAATTAAAACAGCATGGCATCAATTCATGCTTAAACTACCTATCGTGTCTTATTTGGTTAAAACAATTAATGTCGCACG
ATATATTCACACGTTTGGAATTCTATTTGCAGCAGGAGTCAGTGTACTTGAAACCATGCGTGTTTCTTCAAGTTTGGTAA
CCAATATCGTGATGAGACAGGCTTTTGATTTCGCTACTCTACGAGTCCGTGAAGGAAGTGGAATCAGTGAAGCCCTCAAA
GAAACAAAATTCATTAGCCCTATGGCTATCCATCTCATAGCCAGTGGTGAGAAAAGCGGCCAACTTTCAGACATGATGGA
ACGCTCAGCGTCTCATTTAGATAATGAAGTAAACCGACTGATTGAAACATCCTTAACTTTATTAGAACCTATGGTTATTC
TATTAATGGGTGCTGTTGTTTTGTTTATTGTACTTGCGACCTTATTACCTATTTTTTCAATGGAACAATTAGTTGCCTGA

Upstream 100 bases:

>100_bases
TCATCTTTTGTCTCCAGGATAGTCCGCGTATAGTTTACCTTAAGTGACTTATTAATCCCAATAGATTACACTCTATAGTT
TTAATTTTAACAAAAATAAT

Downstream 100 bases:

>100_bases
TTAAGCAGAGGAAGGGTTATCCGCGCCCTTTAAAAATTTAAGTCTATTCATAGGCATCAATATTTGTGTTATAATTTAAC
ATTTAGTTACAAGAGGTACA

Product: type II secretory pathway protein LspF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKSKVSAADLALLTRQLATLLAAGIPVE
ESLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
VQQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTKLLINLSQFIKSYGLYSLVTIIVALIGFKKSLSNI
KIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQAFDFATLRVREGSGISEALK
ETKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA

Sequences:

>Translated_399_residues
MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKSKVSAADLALLTRQLATLLAAGIPVE
ESLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQK
VQQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTKLLINLSQFIKSYGLYSLVTIIVALIGFKKSLSNI
KIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQAFDFATLRVREGSGISEALK
ETKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA
>Mature_398_residues
GAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKSKVSAADLALLTRQLATLLAAGIPVEE
SLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYPNAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQKV
QQALIYPLLMIIVSTAIISFLLTFVVPKIIDVFTESGQTLPPMTKLLINLSQFIKSYGLYSLVTIIVALIGFKKSLSNIK
IKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQAFDFATLRVREGSGISEALKE
TKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRLIETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=395, Percent_Identity=36.7088607594937, Blast_Score=277, Evalue=9e-76,
Organism=Escherichia coli, GI1786295, Length=406, Percent_Identity=26.6009852216749, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43975; Mature: 43844

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKSKVSAA
CCCCEEEEEEECCCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
DLALLTRQLATLLAAGIPVEESLRGVSEQTEKDKVRELIIGVRSKVLEGYGLAQAMAQYP
HHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
NAFPELYRATVGSGEQTGRLDVVLEKLADYTEKQQQTRQKVQQALIYPLLMIIVSTAIIS
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLLTFVVPKIIDVFTESGQTLPPMTKLLINLSQFIKSYGLYSLVTIIVALIGFKKSLSNI
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
KIKTAWHQFMLKLPIVSYLVKTINVARYIHTFGILFAAGVSVLETMRVSSSLVTNIVMRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDFATLRVREGSGISEALKETKFISPMAIHLIASGEKSGQLSDMMERSASHLDNEVNRL
HHHHHHEEEECCCCHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IETSLTLLEPMVILLMGAVVLFIVLATLLPIFSMEQLVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
GAYQYQALKVNGSISKGVIEADSERHARQLLREQGLIPTQVQTLTQQRAASSKSKVSAA
CCCEEEEEEECCCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]