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The map label for this gene is hisF [H]

Identifier: 52841427

GI number: 52841427

Start: 1322810

End: 1323577

Strand: Reverse

Name: hisF [H]

Synonym: lpg1194

Alternate gene names: 52841427

Gene position: 1323577-1322810 (Counterclockwise)

Preceding gene: 52841428

Following gene: 52841426

Centisome position: 38.95

GC content: 39.06

Gene sequence:

>768_bases
ATGTTAACGAAGCGAATCATTCCCTGTCTTGACGTTCGTGATAATCAGGTTGTCAAGGGAGTAAAATTTCGCAACCATAG
AATAATTGGAGAGATACTCCCTTTGGCTGCTCACTACTCAGCATCAGGGGCTGATGAATTAGTATTTTATGATATTACTG
CAAGTAGCGATGACCGATCTGTTTGTCCTGATTGGGTCAATAAAGTAGCGGCTACTATCAATATTCCATTTACAGTGGCT
GGAGGCATCCGAACCTTGCATCAAGCAAGATTAATATTAAATGCCGGAGCAGATAAAATCTCAATCAACAGCCCTGCGTT
GGAAAACCCTTCATTAATTAATGAATTGAGCAAAGAGTTTGGAAAACAATGTATGGTAATAGGAGTTGATAGTCAGTGGA
TAGTTGATGATTATTATGTCTATCAGTATACAGGCAATGAAAATAAAAGTATCAATTCAAAACGCAAAACTCGAGAATGG
ATATATGAAATTCAAGACCGCGGTGCTGGTGAGATAGTCCTTAATTGCATGCAATCTGATGGCGTTCGAATGGGATATGA
TATTGCACAGCTATCAGAAATGATTAACCTCTGCCACGTTCCCCTCATCGCATCGGGCGGTGCTGGAAAAATTGATGATT
TTGTTGAGGTATTTAAAAAGTCTCATGTCAGTGGGGCTTTGGCAGCAAGCGTTTTTCATGACAAAATACTGGCAATCAAT
GACATAAAACTAGCATTACAAACAGAAAATATTGAGGTAAGACTATGA

Upstream 100 bases:

>100_bases
GCCATATGCAGGATATTACAACGCTGAATTCATTAGGAATATCCGCCGTAATCCTTGGGCTAATGCTTTATCAAGATCAT
ATGAATTTAGAGGAATTATT

Downstream 100 bases:

>100_bases
GTGACTTGGACATTAACTCTCTTGATTGGCAAAAAATGAATGGACTATTGCCGGCAATAATCCAAAACGCAGAAAATGGT
AAAGTATTGATGTTAGGTTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGEILPLAAHYSASGADELVFYDITASSDDRSVCPDWVNKVAATINIPFTVA
GGIRTLHQARLILNAGADKISINSPALENPSLINELSKEFGKQCMVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREW
IYEIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVFKKSHVSGALAASVFHDKILAIN
DIKLALQTENIEVRL

Sequences:

>Translated_255_residues
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGEILPLAAHYSASGADELVFYDITASSDDRSVCPDWVNKVAATINIPFTVA
GGIRTLHQARLILNAGADKISINSPALENPSLINELSKEFGKQCMVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREW
IYEIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVFKKSHVSGALAASVFHDKILAIN
DIKLALQTENIEVRL
>Mature_255_residues
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGEILPLAAHYSASGADELVFYDITASSDDRSVCPDWVNKVAATINIPFTVA
GGIRTLHQARLILNAGADKISINSPALENPSLINELSKEFGKQCMVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREW
IYEIQDRGAGEIVLNCMQSDGVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVFKKSHVSGALAASVFHDKILAIN
DIKLALQTENIEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=58.3657587548638, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI87082028, Length=231, Percent_Identity=24.2424242424242, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=30.5031446540881, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28238; Mature: 28238

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGEILPLAAHYSASGADELVFYDITASSDDRS
CCCCCCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
VCPDWVNKVAATINIPFTVAGGIRTLHQARLILNAGADKISINSPALENPSLINELSKEF
CCHHHHHHEEEEEECCEEECCHHHHHHHHHHHEECCCCEEEECCCCCCCCHHHHHHHHHH
GKQCMVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREWIYEIQDRGAGEIVLNCMQSD
CCEEEEEECCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHCC
GVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVFKKSHVSGALAASVFHDKILAIN
CCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCEEEEE
DIKLALQTENIEVRL
EEEEEEEECCEEEEC
>Mature Secondary Structure
MLTKRIIPCLDVRDNQVVKGVKFRNHRIIGEILPLAAHYSASGADELVFYDITASSDDRS
CCCCCCCCCCCCCCCCEEECEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
VCPDWVNKVAATINIPFTVAGGIRTLHQARLILNAGADKISINSPALENPSLINELSKEF
CCHHHHHHEEEEEECCEEECCHHHHHHHHHHHEECCCCEEEECCCCCCCCHHHHHHHHHH
GKQCMVIGVDSQWIVDDYYVYQYTGNENKSINSKRKTREWIYEIQDRGAGEIVLNCMQSD
CCEEEEEECCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHCC
GVRMGYDIAQLSEMINLCHVPLIASGGAGKIDDFVEVFKKSHVSGALAASVFHDKILAIN
CCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCEEEEE
DIKLALQTENIEVRL
EEEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA