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The map label for this gene is yafV [C]

Identifier: 52841185

GI number: 52841185

Start: 1031851

End: 1032657

Strand: Direct

Name: yafV [C]

Synonym: lpg0950

Alternate gene names: 52841185

Gene position: 1031851-1032657 (Clockwise)

Preceding gene: 52841183

Following gene: 52841186

Centisome position: 30.37

GC content: 40.27

Gene sequence:

>807_bases
ATGAATCGAGTAGCGCTTGTGCAAATGGTATCATCTGCCAAAATAGCAGATAATTTGCAACTCACTGAACAATATTTAAT
TGAAGCCAGGGCACAGGAAGCCAGTTTGGTTGTTCTACCTGAGAATTTTGCTTTTATGGGAATGAATGAACGAGAGAAGT
TGCAGATTGCCGAATATTATGGTCAAGGACCTATACAACAAAGAATAAGTGAGTTAGCGAGAGATTTGCGGGTATGGATA
ATAGCAGGAACAATCCCGTTAAAAAGTATGGGTTCAAAGGTAAGAGCAAGTTGTATAGTTTATGATGATAAAGGCCTAAA
TGTTGCTCGTTATGATAAAATTCATTTATTTGATGTCATAGTTTCCGAGCAGGAAAAACACCAGGAATCGTTAACTATAG
AAGCAGGAAAAGATATAGCGCTTGTGGATACGCCAGTGGGGAAAATTGGGTTAACCATCTGTTATGACTTGCGTTTTCCG
GAATTGTACCAATATTTAACCCAAAGAGGCGCACAATTACTTTCAGTTCCGTCTGCTTTTACTGCGGTAACTGGAGCGGC
GCATTGGGAGGTCTTACTCAGAGCAAGAGCAATCGAAAATTTATGTTATGTCTTAGCTCCTAATCAGGGTGGGACTCATG
AAAACGGGCGTCATACTTATGGGCATAGTATGGTGGTGGAACCATGGGGTAAAGTGTTGGCACAAAAAGAAGAAGGGCAG
GGAGTAATTCTGGCTGATATTGATTTAGAGAGATTGTCACAGTTGAGAAGACAATTTCCGTGTGTTGAACATCATGTTTT
AATGTAA

Upstream 100 bases:

>100_bases
TTTATTATTAAGGCGAAAAGGTTTTTGTGTCAATTTTGTTTTGGGCTTATATGGAACGATATAGTAAGATGAAAAATATG
AATGTCATTTAGGAAAAAGG

Downstream 100 bases:

>100_bases
AAGGTATATAAATGACAAAATCTCTTGCGATAGCAAAAAATATTTTATTGACACCAGCTTCCCTGGATGAAGTCGGCATA
GAAAAATTATTCAAGACCAT

Product: nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNRVALVQMVSSAKIADNLQLTEQYLIEARAQEASLVVLPENFAFMGMNEREKLQIAEYYGQGPIQQRISELARDLRVWI
IAGTIPLKSMGSKVRASCIVYDDKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
ELYQYLTQRGAQLLSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRHTYGHSMVVEPWGKVLAQKEEGQ
GVILADIDLERLSQLRRQFPCVEHHVLM

Sequences:

>Translated_268_residues
MNRVALVQMVSSAKIADNLQLTEQYLIEARAQEASLVVLPENFAFMGMNEREKLQIAEYYGQGPIQQRISELARDLRVWI
IAGTIPLKSMGSKVRASCIVYDDKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
ELYQYLTQRGAQLLSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRHTYGHSMVVEPWGKVLAQKEEGQ
GVILADIDLERLSQLRRQFPCVEHHVLM
>Mature_268_residues
MNRVALVQMVSSAKIADNLQLTEQYLIEARAQEASLVVLPENFAFMGMNEREKLQIAEYYGQGPIQQRISELARDLRVWI
IAGTIPLKSMGSKVRASCIVYDDKGLNVARYDKIHLFDVIVSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFP
ELYQYLTQRGAQLLSVPSAFTAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRHTYGHSMVVEPWGKVLAQKEEGQ
GVILADIDLERLSQLRRQFPCVEHHVLM

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=266, Percent_Identity=42.8571428571429, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI297632348, Length=266, Percent_Identity=42.8571428571429, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI297632350, Length=266, Percent_Identity=42.8571428571429, Blast_Score=207, Evalue=6e-54,
Organism=Homo sapiens, GI9910460, Length=261, Percent_Identity=38.6973180076628, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=36.4583333333333, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1786412, Length=227, Percent_Identity=30.8370044052863, Blast_Score=84, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17556280, Length=264, Percent_Identity=42.0454545454545, Blast_Score=211, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6322335, Length=292, Percent_Identity=35.2739726027397, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323383, Length=268, Percent_Identity=34.7014925373134, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17933642, Length=263, Percent_Identity=40.3041825095057, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI21355835, Length=260, Percent_Identity=35.3846153846154, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30087; Mature: 30087

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVALVQMVSSAKIADNLQLTEQYLIEARAQEASLVVLPENFAFMGMNEREKLQIAEYY
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCHHHHHHHHHH
GQGPIQQRISELARDLRVWIIAGTIPLKSMGSKVRASCIVYDDKGLNVARYDKIHLFDVI
CCCHHHHHHHHHHHHEEEEEEECCCCHHHCCCCCEEEEEEEECCCCCEEHCCCEEEEHHH
VSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFPELYQYLTQRGAQLLSVPSAF
HCCHHHHHHCEEEECCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCHHEECCCCHH
TAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRHTYGHSMVVEPWGKVLAQKEEGQ
HHHHCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEECCCCEEECCHHHHHHCCCCCC
GVILADIDLERLSQLRRQFPCVEHHVLM
EEEEEECCHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MNRVALVQMVSSAKIADNLQLTEQYLIEARAQEASLVVLPENFAFMGMNEREKLQIAEYY
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCHHHHHHHHHH
GQGPIQQRISELARDLRVWIIAGTIPLKSMGSKVRASCIVYDDKGLNVARYDKIHLFDVI
CCCHHHHHHHHHHHHEEEEEEECCCCHHHCCCCCEEEEEEEECCCCCEEHCCCEEEEHHH
VSEQEKHQESLTIEAGKDIALVDTPVGKIGLTICYDLRFPELYQYLTQRGAQLLSVPSAF
HCCHHHHHHCEEEECCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCHHEECCCCHH
TAVTGAAHWEVLLRARAIENLCYVLAPNQGGTHENGRHTYGHSMVVEPWGKVLAQKEEGQ
HHHHCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEECCCCEEECCHHHHHHCCCCCC
GVILADIDLERLSQLRRQFPCVEHHVLM
EEEEEECCHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]