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The map label for this gene is dapE

Identifier: 52841122

GI number: 52841122

Start: 959936

End: 961069

Strand: Reverse

Name: dapE

Synonym: lpg0887

Alternate gene names: 52841122

Gene position: 961069-959936 (Counterclockwise)

Preceding gene: 52841123

Following gene: 52841119

Centisome position: 28.29

GC content: 41.18

Gene sequence:

>1134_bases
ATGACTGATATTAAGCAAATTCTGACTGACCTCATTGGTTTTCCTTCTATTACACCAGAAGATGCTGGCTGCCAAAAATA
CATGATTCAATTTCTGGAGCAATTGGGATTTACTTGTCAACAACTGAATAATGGGCCCGTATCCAATTTTTTTGCCTGCT
ATGGCAAAATTGGGCCTTTATTGGTGTTCGCAGGCCATACCGATGTAGTTCCTGTTGGTGAAGTATCAAAATGGGATACA
GACCCTTTCTCTCTCGAAGAAAAAAGTGGCGTGCTCTATGGTCGTGGGGTTGCTGATATGAAAGGCAGCCTGGCCTGTAT
GTTGCATATGGCCAGAAGATTTATTAAAACTTATCCTTCCTTTCCAGGCAGACTAGGATTTCTGATCACCAGTGGCGAAG
AAGGCGATGAATTTAATTTGGGCACCCCTTATGTGATGCAAAAACTGGAGCAGCAAGGGATTGTTATTGATTATTGCATA
GTAGGTGAACCTTCAAGCAGCTTGAAAGCTGGTGATGTCATCAAAATTGGAAGGCGTGGATCATTAAGTGCCAAAATACA
TCTGAGCGGAAAGCAAGGCCATGTTGCCTATCCGCATTTGGCTGACAATCCAATTCATAGAATTAGCCCAGTATTAGCCG
AACTCACTTCAATGCAATGGGATAATGGCAATGCTTTTTTTCCCCCTACCTCCATGCAAATAACTTATATTCATTGTGGA
GGGCATGCGGGTAATATTATCCCAGGCGAATTAAATCTGCATTTGAACTTTAGATATTCGACAGAGCAAACAGACGAGTC
GTTAAAAACGAGAGTAATTAATGCCTTTACACACCATAATCTTAACCCCGCTATAGAATGGCGATTAAATGGCGAACCAT
TCTTAACGAATAAAGGCATATTGCTAGAAAGCTGCAAACAAACAGTGCTTGAACATATTGGGACTCTCCCCGAACTTTCA
ACAAGTGGTGGAACTTCAGATGGGCGTTTTATAGCACCTTATGGGGTTGAGGTCATAGAGCTGGGACTGGTAAATGCAAC
CATTCATCAGGTTAATGAATGCACTTCTCTACAGGATTTGAATACACTTGAAACCATGTATTTTTCAATCTGTGAGAAAT
TGTTAATTGACTAA

Upstream 100 bases:

>100_bases
GATGGAAGCCACAGTCTATATTGTGCTGTCATTGTGAAACAAGTGGATGAAAAAACTCGCGCGAAGGTAAGCATTAATGA
TTTACTAAGAGCCAATCAAG

Downstream 100 bases:

>100_bases
TCAAGCCAATTCTGTAATGAGGGTTCGCATCAACAGTCCAAATTACAGTAATTGTTTAGTGCCCTAATCTTTAAATATCC
TCTAGTGGTCGGATTTGAGT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTDIKQILTDLIGFPSITPEDAGCQKYMIQFLEQLGFTCQQLNNGPVSNFFACYGKIGPLLVFAGHTDVVPVGEVSKWDT
DPFSLEEKSGVLYGRGVADMKGSLACMLHMARRFIKTYPSFPGRLGFLITSGEEGDEFNLGTPYVMQKLEQQGIVIDYCI
VGEPSSSLKAGDVIKIGRRGSLSAKIHLSGKQGHVAYPHLADNPIHRISPVLAELTSMQWDNGNAFFPPTSMQITYIHCG
GHAGNIIPGELNLHLNFRYSTEQTDESLKTRVINAFTHHNLNPAIEWRLNGEPFLTNKGILLESCKQTVLEHIGTLPELS
TSGGTSDGRFIAPYGVEVIELGLVNATIHQVNECTSLQDLNTLETMYFSICEKLLID

Sequences:

>Translated_377_residues
MTDIKQILTDLIGFPSITPEDAGCQKYMIQFLEQLGFTCQQLNNGPVSNFFACYGKIGPLLVFAGHTDVVPVGEVSKWDT
DPFSLEEKSGVLYGRGVADMKGSLACMLHMARRFIKTYPSFPGRLGFLITSGEEGDEFNLGTPYVMQKLEQQGIVIDYCI
VGEPSSSLKAGDVIKIGRRGSLSAKIHLSGKQGHVAYPHLADNPIHRISPVLAELTSMQWDNGNAFFPPTSMQITYIHCG
GHAGNIIPGELNLHLNFRYSTEQTDESLKTRVINAFTHHNLNPAIEWRLNGEPFLTNKGILLESCKQTVLEHIGTLPELS
TSGGTSDGRFIAPYGVEVIELGLVNATIHQVNECTSLQDLNTLETMYFSICEKLLID
>Mature_376_residues
TDIKQILTDLIGFPSITPEDAGCQKYMIQFLEQLGFTCQQLNNGPVSNFFACYGKIGPLLVFAGHTDVVPVGEVSKWDTD
PFSLEEKSGVLYGRGVADMKGSLACMLHMARRFIKTYPSFPGRLGFLITSGEEGDEFNLGTPYVMQKLEQQGIVIDYCIV
GEPSSSLKAGDVIKIGRRGSLSAKIHLSGKQGHVAYPHLADNPIHRISPVLAELTSMQWDNGNAFFPPTSMQITYIHCGG
HAGNIIPGELNLHLNFRYSTEQTDESLKTRVINAFTHHNLNPAIEWRLNGEPFLTNKGILLESCKQTVLEHIGTLPELST
SGGTSDGRFIAPYGVEVIELGLVNATIHQVNECTSLQDLNTLETMYFSICEKLLID

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=372, Percent_Identity=49.4623655913978, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1789236, Length=218, Percent_Identity=26.1467889908257, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790395, Length=270, Percent_Identity=28.8888888888889, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551016, Length=270, Percent_Identity=28.1481481481481, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17563146, Length=239, Percent_Identity=25.1046025104602, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_LEGPA (Q5X6L7)

Other databases:

- EMBL:   CR628336
- RefSeq:   YP_123276.1
- ProteinModelPortal:   Q5X6L7
- SMR:   Q5X6L7
- STRING:   Q5X6L7
- MEROPS:   M20.010
- GeneID:   3119485
- GenomeReviews:   CR628336_GR
- KEGG:   lpp:lpp0948
- LegioList:   lpp0948
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- BioCyc:   LPNE297246:LPP0948-MONOMER
- HAMAP:   MF_01690
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41483; Mature: 41352

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 68-68 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIKQILTDLIGFPSITPEDAGCQKYMIQFLEQLGFTCQQLNNGPVSNFFACYGKIGPL
CCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCCE
LVFAGHTDVVPVGEVSKWDTDPFSLEEKSGVLYGRGVADMKGSLACMLHMARRFIKTYPS
EEECCCCCCEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
FPGRLGFLITSGEEGDEFNLGTPYVMQKLEQQGIVIDYCIVGEPSSSLKAGDVIKIGRRG
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCC
SLSAKIHLSGKQGHVAYPHLADNPIHRISPVLAELTSMQWDNGNAFFPPTSMQITYIHCG
CCEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHCEECCCCEECCCCCCEEEEEEEC
GHAGNIIPGELNLHLNFRYSTEQTDESLKTRVINAFTHHNLNPAIEWRLNGEPFLTNKGI
CCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCH
LLESCKQTVLEHIGTLPELSTSGGTSDGRFIAPYGVEVIELGLVNATIHQVNECTSLQDL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCEEEEEEHHHHHHHHHHHHCCHHHH
NTLETMYFSICEKLLID
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDIKQILTDLIGFPSITPEDAGCQKYMIQFLEQLGFTCQQLNNGPVSNFFACYGKIGPL
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCCE
LVFAGHTDVVPVGEVSKWDTDPFSLEEKSGVLYGRGVADMKGSLACMLHMARRFIKTYPS
EEECCCCCCEECCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCC
FPGRLGFLITSGEEGDEFNLGTPYVMQKLEQQGIVIDYCIVGEPSSSLKAGDVIKIGRRG
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCC
SLSAKIHLSGKQGHVAYPHLADNPIHRISPVLAELTSMQWDNGNAFFPPTSMQITYIHCG
CCEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHCEECCCCEECCCCCCEEEEEEEC
GHAGNIIPGELNLHLNFRYSTEQTDESLKTRVINAFTHHNLNPAIEWRLNGEPFLTNKGI
CCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEECCCH
LLESCKQTVLEHIGTLPELSTSGGTSDGRFIAPYGVEVIELGLVNATIHQVNECTSLQDL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCEEEEEEHHHHHHHHHHHHCCHHHH
NTLETMYFSICEKLLID
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA