Click here to switch to the map view.

The map label for this gene is mesJ

Identifier: 52841023

GI number: 52841023

Start: 860039

End: 861340

Strand: Direct

Name: mesJ

Synonym: lpg0786

Alternate gene names: 52841023

Gene position: 860039-861340 (Clockwise)

Preceding gene: 52841022

Following gene: 52841024

Centisome position: 25.31

GC content: 40.02

Gene sequence:

>1302_bases
GTGCTTGTGACTAGGCCTTTACTAAGTCCCGAATGGGTAGCTCGCCTTAAACAATTCAAGAAATTGATAGTAGGGTTCAG
CGGTGGTCTGGACTCTACAGTGCTCCTGCATGTCCTTGCCTCAACTCCTCCCTTGTCTAATCAACTTTTGGCTGTCCATA
TTAACCATGGCATTAGTGGAAATTCTCTGAACTGGCAAAGGCATTGTGAGCAATTGTGCCTGGATTTAGGTATTGCTTTT
ATTGCCAAAGCGATTGAATTTGATCGTTCTGCAAATGTTGAAGAAGCAGCACGCAATGCGAGATATGATTTTTTTTCCTC
CTTATTAGAGGATAATGATTGTTTGGTTTTGGGGCATCATCTTGATGATCAGGCTGAAACAGTTCTTTTGCAATTGTTTC
GCGGTGCAGGAGTTGATGGCTTGGCAGCTATGCAGGAATGCAGCAATTTGGGTGCTGGGCAATTGGCAAGACCATTTTTG
ACCTGCCCCAGAGTGGAATTAGAGCATTATGCTCGTATTCATGAATTGAAATGGATAGAAGACGAAAGCAATCAAGATAC
AAAATATTCTCGCAATTATCTGCGTCATCGCGTAATGCCATTATTGCTTGAAAAGTGGCCGGGAGTAGCAGGTAATATTT
CCCGAGCTGCGACTCATTGTCAGCAGGCAAAAGCGAATCTTGAGGTATTGGCTATAAAGGATTGTCCTGATTTGTTAAAC
GCTACTGATCATTTGCTGATTGAACCCATAAAAGGACTTGAATTTGAGCGGATTACAAATGTTTTAAAGTTTTGGCTTAA
AAAAAATAGAATTCAATTGCCTTCGTCCAAGACGTTTCATCGTATCATTCATGAAATAATTTTTGCAAAGCTCGATGCTA
TGCCAACAGTGAGTTGGAATCAAATACAAGTACGTCGTTTCCAACAACATCTTTATTTGCTTAAGGCAGACCAGATCAAT
TTACCGAAGGCTATTAAATGGCAACAATTTCCAGCTTCTTTAACTTATCCAGACGCTGATATTAAGTTGTCTGCAGTAAA
GGCCCAAAAGGGATTAATGATCCCGAAAGATGCGCAAATAGAAATACGATTCAGAAAGGGTGGTGAAGAGATTTATTGGC
ATGGTCAAACCAAACATCTAAAAAAACTATTTCAGGAATGGCAAATTCCCCCATGGGTAAGGGATAGAGTTCCATTGGTT
TACATTAATGATCAATTGGCTTGTGTAGTAGGTTATGCAGTGAGCGATTTGTTTTTTACTACTAATCCACTGGAAGCTTG
GTCTATAGTTAATAATTCTTAA

Upstream 100 bases:

>100_bases
AGTCGCTTGAAGAGATTATTAACGAGTGAACTCCAGGCTTTGAAAAAGGTGCCTCTGGATCAATTAATTGAGTTAAGGTA
TCAAAAGTTTATGGCAATGG

Downstream 100 bases:

>100_bases
TATAACAATCTCCGCTCCCTTACGGTCGCGGCTCGGTTTTGACTATAATTCCATTTGCACTCCCTTATGGTCGTAGCTCG
GTTTTGACTATAATTCCATT

Product: cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLVTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLSNQLLAVHINHGISGNSLNWQRHCEQLCLDLGIAF
IAKAIEFDRSANVEEAARNARYDFFSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFL
TCPRVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHCQQAKANLEVLAIKDCPDLLN
ATDHLLIEPIKGLEFERITNVLKFWLKKNRIQLPSSKTFHRIIHEIIFAKLDAMPTVSWNQIQVRRFQQHLYLLKADQIN
LPKAIKWQQFPASLTYPDADIKLSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHGQTKHLKKLFQEWQIPPWVRDRVPLV
YINDQLACVVGYAVSDLFFTTNPLEAWSIVNNS

Sequences:

>Translated_433_residues
MLVTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLSNQLLAVHINHGISGNSLNWQRHCEQLCLDLGIAF
IAKAIEFDRSANVEEAARNARYDFFSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFL
TCPRVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHCQQAKANLEVLAIKDCPDLLN
ATDHLLIEPIKGLEFERITNVLKFWLKKNRIQLPSSKTFHRIIHEIIFAKLDAMPTVSWNQIQVRRFQQHLYLLKADQIN
LPKAIKWQQFPASLTYPDADIKLSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHGQTKHLKKLFQEWQIPPWVRDRVPLV
YINDQLACVVGYAVSDLFFTTNPLEAWSIVNNS
>Mature_433_residues
MLVTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLSNQLLAVHINHGISGNSLNWQRHCEQLCLDLGIAF
IAKAIEFDRSANVEEAARNARYDFFSSLLEDNDCLVLGHHLDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFL
TCPRVELEHYARIHELKWIEDESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHCQQAKANLEVLAIKDCPDLLN
ATDHLLIEPIKGLEFERITNVLKFWLKKNRIQLPSSKTFHRIIHEIIFAKLDAMPTVSWNQIQVRRFQQHLYLLKADQIN
LPKAIKWQQFPASLTYPDADIKLSAVKAQKGLMIPKDAQIEIRFRKGGEEIYWHGQTKHLKKLFQEWQIPPWVRDRVPLV
YINDQLACVVGYAVSDLFFTTNPLEAWSIVNNS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=401, Percent_Identity=35.1620947630923, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_LEGPH (Q5ZXE5)

Other databases:

- EMBL:   AE017354
- RefSeq:   YP_094822.1
- ProteinModelPortal:   Q5ZXE5
- SMR:   Q5ZXE5
- STRING:   Q5ZXE5
- GeneID:   3079263
- GenomeReviews:   AE017354_GR
- KEGG:   lpn:lpg0786
- eggNOG:   COG0037
- HOGENOM:   HBG668702
- OMA:   KSAVGFF
- ProtClustDB:   CLSK832407
- BioCyc:   LPNE272624:LPG0786-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 49413; Mature: 49413

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLSNQLLAVHINHGISG
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCC
NSLNWQRHCEQLCLDLGIAFIAKAIEFDRSANVEEAARNARYDFFSSLLEDNDCLVLGHH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHCCCCEEEEECC
LDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTCPRVELEHYARIHELKWIE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHEEEC
DESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHCQQAKANLEVLAIKDCPDLLN
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHC
ATDHLLIEPIKGLEFERITNVLKFWLKKNRIQLPSSKTFHRIIHEIIFAKLDAMPTVSWN
CCCCEECCCCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCH
QIQVRRFQQHLYLLKADQINLPKAIKWQQFPASLTYPDADIKLSAVKAQKGLMIPKDAQI
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCEE
EIRFRKGGEEIYWHGQTKHLKKLFQEWQIPPWVRDRVPLVYINDQLACVVGYAVSDLFFT
EEEEECCCCEEEECCCHHHHHHHHHHCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHCC
TNPLEAWSIVNNS
CCCCHHHHCCCCC
>Mature Secondary Structure
MLVTRPLLSPEWVARLKQFKKLIVGFSGGLDSTVLLHVLASTPPLSNQLLAVHINHGISG
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCC
NSLNWQRHCEQLCLDLGIAFIAKAIEFDRSANVEEAARNARYDFFSSLLEDNDCLVLGHH
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHCCCCEEEEECC
LDDQAETVLLQLFRGAGVDGLAAMQECSNLGAGQLARPFLTCPRVELEHYARIHELKWIE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHEEEC
DESNQDTKYSRNYLRHRVMPLLLEKWPGVAGNISRAATHCQQAKANLEVLAIKDCPDLLN
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHC
ATDHLLIEPIKGLEFERITNVLKFWLKKNRIQLPSSKTFHRIIHEIIFAKLDAMPTVSWN
CCCCEECCCCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCH
QIQVRRFQQHLYLLKADQINLPKAIKWQQFPASLTYPDADIKLSAVKAQKGLMIPKDAQI
HHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCCEE
EIRFRKGGEEIYWHGQTKHLKKLFQEWQIPPWVRDRVPLVYINDQLACVVGYAVSDLFFT
EEEEECCCCEEEECCCHHHHHHHHHHCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHCC
TNPLEAWSIVNNS
CCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA