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The map label for this gene is artJ [H]

Identifier: 52840915

GI number: 52840915

Start: 726983

End: 727747

Strand: Reverse

Name: artJ [H]

Synonym: lpg0678

Alternate gene names: 52840915

Gene position: 727747-726983 (Counterclockwise)

Preceding gene: 52840922

Following gene: 52840913

Centisome position: 21.42

GC content: 38.43

Gene sequence:

>765_bases
GTGAAGATACCAATGAAATTTATTCAGGGATTATTGCTTTCTTTCCTTTTTCTAGTTTCACCTCTCCACGCTCAAAACGA
GCCTGTACCTTTAATAGTAGGTGTAGGAGGCTTTTACCCTCCCTTTATCATGCAAGGAACCAATAATGAACTCTTTGGTT
ATGACATCGCAACCATGACAGAGCTGTGTAAGATAATGAAACGCCAATGTAATTTTCGAGTCATGAGATTCAGACAATTA
ATTCCTGCTCTGATTAACAAACAAGTTGATGCAGCGATCAGCGCCATATCTATTACTTCAGAACGCGCAAGCCTGGTTAA
TTTCAGTATGCCCTATTTATTGAGTTACTCCAGATTTCTTACAAATAATATACACAACACTCATCAACCGTTTAATTTAA
TGCTCTTAAAAGGTAAACGAATCGGTGTAGAAGAAAATACGGTGTTTAGCCAAGAAATCAGGAGGATGGGGATAATAGAC
CCTGTGATAGTCGAATTTGACAGAACGGAAGAGATGCTTGAGGCCTTAAATGCAAATGAAGTTGATTTTGTTCTGATGGA
TAATCCTGCTGCCTTATACTGGTCTGCTAATTCGTCCGGAAGGCTTCTGGTGGTTGGTAGTCCCATGCTGGTTGGTTATG
GTTTGGGCATTGCAGTAAATCGGGAAAATGCTGAATTACTCAAATCGCTTAATGACGCATTAATAGAATACCAGCGTTCA
GAAAAATACAAGGAAAATTACAACAAATACATGATGAATTTTTAA

Upstream 100 bases:

>100_bases
ACCTAAACATATCATAGGATTAATCAATTAATTTTACATTTAATACTTTAAAACTCCTATTGGACGTTATACTCTTATAA
AAGACTCTAATAATATTTTA

Downstream 100 bases:

>100_bases
GAGTTCAATGTTTGGCAATTGCTGCAAAAATTGCCCTTAAAAATTCATCAATAACTAAATAATTGTTTTAATACGTAATA
GGTTAGTGCTTTTATGTTTA

Product: arginine ABC transporter, periplasmic binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKIPMKFIQGLLLSFLFLVSPLHAQNEPVPLIVGVGGFYPPFIMQGTNNELFGYDIATMTELCKIMKRQCNFRVMRFRQL
IPALINKQVDAAISAISITSERASLVNFSMPYLLSYSRFLTNNIHNTHQPFNLMLLKGKRIGVEENTVFSQEIRRMGIID
PVIVEFDRTEEMLEALNANEVDFVLMDNPAALYWSANSSGRLLVVGSPMLVGYGLGIAVNRENAELLKSLNDALIEYQRS
EKYKENYNKYMMNF

Sequences:

>Translated_254_residues
MKIPMKFIQGLLLSFLFLVSPLHAQNEPVPLIVGVGGFYPPFIMQGTNNELFGYDIATMTELCKIMKRQCNFRVMRFRQL
IPALINKQVDAAISAISITSERASLVNFSMPYLLSYSRFLTNNIHNTHQPFNLMLLKGKRIGVEENTVFSQEIRRMGIID
PVIVEFDRTEEMLEALNANEVDFVLMDNPAALYWSANSSGRLLVVGSPMLVGYGLGIAVNRENAELLKSLNDALIEYQRS
EKYKENYNKYMMNF
>Mature_254_residues
MKIPMKFIQGLLLSFLFLVSPLHAQNEPVPLIVGVGGFYPPFIMQGTNNELFGYDIATMTELCKIMKRQCNFRVMRFRQL
IPALINKQVDAAISAISITSERASLVNFSMPYLLSYSRFLTNNIHNTHQPFNLMLLKGKRIGVEENTVFSQEIRRMGIID
PVIVEFDRTEEMLEALNANEVDFVLMDNPAALYWSANSSGRLLVVGSPMLVGYGLGIAVNRENAELLKSLNDALIEYQRS
EKYKENYNKYMMNF

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=244, Percent_Identity=33.6065573770492, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787088, Length=218, Percent_Identity=31.1926605504587, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788228, Length=229, Percent_Identity=27.9475982532751, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788649, Length=251, Percent_Identity=28.6852589641434, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=30.1724137931034, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787031, Length=240, Percent_Identity=29.1666666666667, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28951; Mature: 28951

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPMKFIQGLLLSFLFLVSPLHAQNEPVPLIVGVGGFYPPFIMQGTNNELFGYDIATMT
CCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCCCCEECCHHHHHH
ELCKIMKRQCNFRVMRFRQLIPALINKQVDAAISAISITSERASLVNFSMPYLLSYSRFL
HHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHHH
TNNIHNTHQPFNLMLLKGKRIGVEENTVFSQEIRRMGIIDPVIVEFDRTEEMLEALNANE
HCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHEEEECCHHHHHHHCCCCC
VDFVLMDNPAALYWSANSSGRLLVVGSPMLVGYGLGIAVNRENAELLKSLNDALIEYQRS
EEEEEECCCEEEEEECCCCCEEEEEECCCEEEECCEEEEECCHHHHHHHHHHHHHHHHHH
EKYKENYNKYMMNF
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKIPMKFIQGLLLSFLFLVSPLHAQNEPVPLIVGVGGFYPPFIMQGTNNELFGYDIATMT
CCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEECCCCCEECCHHHHHH
ELCKIMKRQCNFRVMRFRQLIPALINKQVDAAISAISITSERASLVNFSMPYLLSYSRFL
HHHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHHH
TNNIHNTHQPFNLMLLKGKRIGVEENTVFSQEIRRMGIIDPVIVEFDRTEEMLEALNANE
HCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHEEEECCHHHHHHHCCCCC
VDFVLMDNPAALYWSANSSGRLLVVGSPMLVGYGLGIAVNRENAELLKSLNDALIEYQRS
EEEEEECCCEEEEEECCCCCEEEEEECCCEEEECCEEEEECCHHHHHHHHHHHHHHHHHH
EKYKENYNKYMMNF
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]