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The map label for this gene is phbC [H]

Identifier: 52840836

GI number: 52840836

Start: 631254

End: 633014

Strand: Reverse

Name: phbC [H]

Synonym: lpg0599

Alternate gene names: 52840836

Gene position: 633014-631254 (Counterclockwise)

Preceding gene: 52840845

Following gene: 52840833

Centisome position: 18.63

GC content: 38.27

Gene sequence:

>1761_bases
TTGCTTTCAGGAGCCAGGATGACTCACGACACTGAACTAAGCGAACTGATGCAGGCTGTAGCTAAAAAAAGCCTGCAGAT
TATGACCGATTTCAAAGAAAAACCCATCCCGATTTCATCGCTGGTCAGTCAATACATCGATCTCACGGAGCATTTCCAAA
ATTTGATTGCCGTCATTCTAAAAAATCCTGAAAAAGTATGGCAAATGCAGTTGAATTACCTAGAAGATGCCTTAAGTCTG
GCTCAAGCACAATTTAATTATTGGCTTGAAGGAAAACCACTGCCTATTAACGATCAGCGTTTTAATGGGGAAGATTGGAT
AAACAATCCTTTTTTTAACTTGCTAAGCCAACATTATCTTTTGGCAAATGAGCATATGAATTCATTGCTGGAAAATATGG
AATATGGTGACGAGAATCTGGCAAAAAGAGTACGTTTTTTCACCCGACAATATCTTGATGCTTTATCCCCAGCTAATTTT
ATTCACACGAATCCTCAGCTCATGGCAGAAACATTACAAAGTCATGGTAAAAACCTATTACGTGGATTACACAATTTGCT
ATCCGATGTTGAAGCAGGATCTTCCAGATTAATCATTAAAATGACTGATACGGAAGCATTTAAAATCGGTGAGAATCTGG
CTACAACGCCTGGTAAAGTCATATTTCGCAATTCCATGATGGAGTTAATCCAATATTGCCCCAGAACAACAAAAGTAAAA
TCCATACCTTTACTTATCGTTCCTCCCTGGATTAATAAATACTACATTTTAGACTTAAGCCCGCATAATTCATTAATACG
CTGGTTGGTCGAACAAGGGATTACTGTTTTTATTATCTCCTGGGTCAACCCTGACGAAACGTATGCCAATAAAAGCTTCT
ATGATTATTTAAATGAAGGCCCCAGAGAAGCCATTGCAGTAATTCAAAAACAATTGCGTGTTAAACAAGTCAATACGCTG
GGATTCTGTATAGGCGGGACACTTCTGGCTTCTCTTTTAGCCTACAATAAGGCAACTAAGGATCATTCCATTCGCAGTGC
AACCTTTTTAGCGGCAATGATTGATTTCAGTGATCCTGGGGACATCGCTGTTTTTATTGATGAGCAGCAGATTAATAAAC
TTGAAGAAGAAATGAAATCCAAAGGTTATCTGGCTGGCAAATTTATGGCGTCCAGCTTTAATTCCTTAAGGGCAAATGAC
CTAATATGGTCATTTTTCATCAAAAATTACCTGCGCGGTAAAAGCCCTGTTCCTTTTGACATACTCTATTGGAATGCTGA
TTCAACTAATATGCCTGCAACCATGCATTCTCAATATTTACGTTGGATGTATTTGCATAATAACTTGATAAAACCTGGAA
AAATTCGCCTTAATCATATCCCTATTGACGTAACAAATATCGACATACCCACTTTCTTTCTGTCTACTCAAAAAGATCAT
ATCGCTCCCTGGAAAACAACATATAAAGGATTTGAATTAATGAAGGGGCCAAAACGTTTTGTTTTAGGTGGATCTGGACA
TATTGCAGGAATTATTAATCCGCCAACCCAGCAAAAATATGGTTATAGAACCAATAACAGCATGGATTTATCTGCTGAGC
AATGGTTTGAAAAATCCAAGGAACACTCCGGTTCATGGTGGCCAGAATGGTTAAATTGGCTTAAATTGCATTCCGGAAGA
CTCATCAATTCCCCTGATATCAATCACTTGCCATTCGCACCGATAATGGATGCTCCAGGGAGTTATGTACTAAAAAAATA
A

Upstream 100 bases:

>100_bases
GGCTTGCCAATATTCCATCTGTCTATAATGTGCTCTGGATTTAATATAGAATACTCTTGATAAACACAACCAAACCCATA
AGAATAAGCTATTCGATTAT

Downstream 100 bases:

>100_bases
TATTTTGAACTCAATAACCATACATTAAAACATAAAAGGAGTTGTATTGTGAGAAAAAAAAACAGGATACTTTTGGCAAG
TTTATTCTTGCTAGGCAGCC

Product: poly-beta-hydroxybutyrate polymerase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MLSGARMTHDTELSELMQAVAKKSLQIMTDFKEKPIPISSLVSQYIDLTEHFQNLIAVILKNPEKVWQMQLNYLEDALSL
AQAQFNYWLEGKPLPINDQRFNGEDWINNPFFNLLSQHYLLANEHMNSLLENMEYGDENLAKRVRFFTRQYLDALSPANF
IHTNPQLMAETLQSHGKNLLRGLHNLLSDVEAGSSRLIIKMTDTEAFKIGENLATTPGKVIFRNSMMELIQYCPRTTKVK
SIPLLIVPPWINKYYILDLSPHNSLIRWLVEQGITVFIISWVNPDETYANKSFYDYLNEGPREAIAVIQKQLRVKQVNTL
GFCIGGTLLASLLAYNKATKDHSIRSATFLAAMIDFSDPGDIAVFIDEQQINKLEEEMKSKGYLAGKFMASSFNSLRAND
LIWSFFIKNYLRGKSPVPFDILYWNADSTNMPATMHSQYLRWMYLHNNLIKPGKIRLNHIPIDVTNIDIPTFFLSTQKDH
IAPWKTTYKGFELMKGPKRFVLGGSGHIAGIINPPTQQKYGYRTNNSMDLSAEQWFEKSKEHSGSWWPEWLNWLKLHSGR
LINSPDINHLPFAPIMDAPGSYVLKK

Sequences:

>Translated_586_residues
MLSGARMTHDTELSELMQAVAKKSLQIMTDFKEKPIPISSLVSQYIDLTEHFQNLIAVILKNPEKVWQMQLNYLEDALSL
AQAQFNYWLEGKPLPINDQRFNGEDWINNPFFNLLSQHYLLANEHMNSLLENMEYGDENLAKRVRFFTRQYLDALSPANF
IHTNPQLMAETLQSHGKNLLRGLHNLLSDVEAGSSRLIIKMTDTEAFKIGENLATTPGKVIFRNSMMELIQYCPRTTKVK
SIPLLIVPPWINKYYILDLSPHNSLIRWLVEQGITVFIISWVNPDETYANKSFYDYLNEGPREAIAVIQKQLRVKQVNTL
GFCIGGTLLASLLAYNKATKDHSIRSATFLAAMIDFSDPGDIAVFIDEQQINKLEEEMKSKGYLAGKFMASSFNSLRAND
LIWSFFIKNYLRGKSPVPFDILYWNADSTNMPATMHSQYLRWMYLHNNLIKPGKIRLNHIPIDVTNIDIPTFFLSTQKDH
IAPWKTTYKGFELMKGPKRFVLGGSGHIAGIINPPTQQKYGYRTNNSMDLSAEQWFEKSKEHSGSWWPEWLNWLKLHSGR
LINSPDINHLPFAPIMDAPGSYVLKK
>Mature_586_residues
MLSGARMTHDTELSELMQAVAKKSLQIMTDFKEKPIPISSLVSQYIDLTEHFQNLIAVILKNPEKVWQMQLNYLEDALSL
AQAQFNYWLEGKPLPINDQRFNGEDWINNPFFNLLSQHYLLANEHMNSLLENMEYGDENLAKRVRFFTRQYLDALSPANF
IHTNPQLMAETLQSHGKNLLRGLHNLLSDVEAGSSRLIIKMTDTEAFKIGENLATTPGKVIFRNSMMELIQYCPRTTKVK
SIPLLIVPPWINKYYILDLSPHNSLIRWLVEQGITVFIISWVNPDETYANKSFYDYLNEGPREAIAVIQKQLRVKQVNTL
GFCIGGTLLASLLAYNKATKDHSIRSATFLAAMIDFSDPGDIAVFIDEQQINKLEEEMKSKGYLAGKFMASSFNSLRAND
LIWSFFIKNYLRGKSPVPFDILYWNADSTNMPATMHSQYLRWMYLHNNLIKPGKIRLNHIPIDVTNIDIPTFFLSTQKDH
IAPWKTTYKGFELMKGPKRFVLGGSGHIAGIINPPTQQKYGYRTNNSMDLSAEQWFEKSKEHSGSWWPEWLNWLKLHSGR
LINSPDINHLPFAPIMDAPGSYVLKK

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 67291; Mature: 67291

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSGARMTHDTELSELMQAVAKKSLQIMTDFKEKPIPISSLVSQYIDLTEHFQNLIAVIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KNPEKVWQMQLNYLEDALSLAQAQFNYWLEGKPLPINDQRFNGEDWINNPFFNLLSQHYL
CCHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LANEHMNSLLENMEYGDENLAKRVRFFTRQYLDALSPANFIHTNPQLMAETLQSHGKNLL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHH
RGLHNLLSDVEAGSSRLIIKMTDTEAFKIGENLATTPGKVIFRNSMMELIQYCPRTTKVK
HHHHHHHHHHCCCCCEEEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
SIPLLIVPPWINKYYILDLSPHNSLIRWLVEQGITVFIISWVNPDETYANKSFYDYLNEG
CCCEEEECCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCHHHCCCHHHHHHCCC
PREAIAVIQKQLRVKQVNTLGFCIGGTLLASLLAYNKATKDHSIRSATFLAAMIDFSDPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
DIAVFIDEQQINKLEEEMKSKGYLAGKFMASSFNSLRANDLIWSFFIKNYLRGKSPVPFD
CEEEEECHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
ILYWNADSTNMPATMHSQYLRWMYLHNNLIKPGKIRLNHIPIDVTNIDIPTFFLSTQKDH
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCCC
IAPWKTTYKGFELMKGPKRFVLGGSGHIAGIINPPTQQKYGYRTNNSMDLSAEQWFEKSK
CCCCHHHHCCHHHHCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
EHSGSWWPEWLNWLKLHSGRLINSPDINHLPFAPIMDAPGSYVLKK
HCCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLSGARMTHDTELSELMQAVAKKSLQIMTDFKEKPIPISSLVSQYIDLTEHFQNLIAVIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KNPEKVWQMQLNYLEDALSLAQAQFNYWLEGKPLPINDQRFNGEDWINNPFFNLLSQHYL
CCHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LANEHMNSLLENMEYGDENLAKRVRFFTRQYLDALSPANFIHTNPQLMAETLQSHGKNLL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHH
RGLHNLLSDVEAGSSRLIIKMTDTEAFKIGENLATTPGKVIFRNSMMELIQYCPRTTKVK
HHHHHHHHHHCCCCCEEEEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
SIPLLIVPPWINKYYILDLSPHNSLIRWLVEQGITVFIISWVNPDETYANKSFYDYLNEG
CCCEEEECCCCCEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCHHHCCCHHHHHHCCC
PREAIAVIQKQLRVKQVNTLGFCIGGTLLASLLAYNKATKDHSIRSATFLAAMIDFSDPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCC
DIAVFIDEQQINKLEEEMKSKGYLAGKFMASSFNSLRANDLIWSFFIKNYLRGKSPVPFD
CEEEEECHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
ILYWNADSTNMPATMHSQYLRWMYLHNNLIKPGKIRLNHIPIDVTNIDIPTFFLSTQKDH
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCCC
IAPWKTTYKGFELMKGPKRFVLGGSGHIAGIINPPTQQKYGYRTNNSMDLSAEQWFEKSK
CCCCHHHHCCHHHHCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
EHSGSWWPEWLNWLKLHSGRLINSPDINHLPFAPIMDAPGSYVLKK
HCCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]