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The map label for this gene is ytbE

Identifier: 52840762

GI number: 52840762

Start: 558434

End: 559264

Strand: Reverse

Name: ytbE

Synonym: lpg0517

Alternate gene names: 52840762

Gene position: 559264-558434 (Counterclockwise)

Preceding gene: 52840763

Following gene: 52840754

Centisome position: 16.46

GC content: 36.7

Gene sequence:

>831_bases
ATGGACCTTGGCTTTCCAACTTTTATATATGGAACCGCCTGGAAAGAAAAGCGTACAAGCGAACTGGTCGAGCTTGCATT
AACTACCGGATTTCAGGGTATAGATACTGCAAACCAAAGAAAACACTATTATGAAGAAGGTGTTGGATATGCCTTGCAAC
AATACTATAAAAAATCAGGTATCTTGCGTGATGATATTTTTTTACAATCCAAGTTCACCTATACTCATGGCCAGGATCAT
CGCAAACCTTACGATGATACAGCAAGTCTGACTATTCAAGTCCAGCAATCTTTTGCAAGCAGCCTTGAACATTTAAATAC
TGATTATTTAAATTCCTATATTTTGCATGGCCCTTATTATCAATATCATTTTTCTGATGAAGATAAAGAAGTCTGGAACA
AAATGGAATCTCTGCATAAAGATGGGAAAGTAAAACACCTGGGCATATCGAATGTGAGTTATGAACAGCTAGTCGAACTC
ATTTCATTTGCTTCGGTAAAACCCAAGTTTGTTCAAAATAGATGCTTTGCCAGGCTATTATGGGATAAACAAATTCGTAA
CTATTGCCAGGCTCATGGAATTATTTATCAGGGGTTTTCTCTGTTAACGGCTAATCAATATGAGTTAAGGCATCCGAAAC
TTGAGAATCTCAGCAAAAAGCACGAGAAATCGATTGAGCAAATCATTTTTAGATTTTCACAACAGATAGGAATGTTGCCT
TTAACTGGCACGACAAATTTGGATCATATGAAAGAAGATCTTGCCATCCATCAATTCAGGCTGACTGATGATGAAATCAA
TTTCATTGAAAACATTGCCTTTATCCCATAA

Upstream 100 bases:

>100_bases
AAAACCCTATGCAAGACACACAGTCAGCTATGGTTTGCTCTGTTTTTTGAGTCAAAATAGAGTCTGTTTGCAGATAATAA
ATCGAGAAGGAGATTGAACG

Downstream 100 bases:

>100_bases
AATTTTTTTTCGATTTGACTGGGAAATTCATATTGTAATCCCCTGTAAAATGGCATTCCCTAACCAAAAAAAATGAACTT
CATGATGAATGCTTCCTGAC

Product: aldo/keto reductases

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYYEEGVGYALQQYYKKSGILRDDIFLQSKFTYTHGQDH
RKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLVEL
ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLSKKHEKSIEQIIFRFSQQIGMLP
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP

Sequences:

>Translated_276_residues
MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYYEEGVGYALQQYYKKSGILRDDIFLQSKFTYTHGQDH
RKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLVEL
ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLSKKHEKSIEQIIFRFSQQIGMLP
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP
>Mature_276_residues
MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYYEEGVGYALQQYYKKSGILRDDIFLQSKFTYTHGQDH
RKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYYQYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLVEL
ISFASVKPKFVQNRCFARLLWDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLSKKHEKSIEQIIFRFSQQIGMLP
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=27.3037542662116, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI291291012, Length=280, Percent_Identity=26.7857142857143, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI45446745, Length=298, Percent_Identity=25.8389261744966, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI4503285, Length=298, Percent_Identity=25.8389261744966, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=26.5993265993266, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI310109920, Length=298, Percent_Identity=25.503355704698, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI5453543, Length=298, Percent_Identity=25.503355704698, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI24497583, Length=298, Percent_Identity=25.1677852348993, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI5174695, Length=304, Percent_Identity=25.3289473684211, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI310109922, Length=283, Percent_Identity=25.7950530035336, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=25, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=25, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI93277124, Length=301, Percent_Identity=24.9169435215947, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI300116273, Length=283, Percent_Identity=24.0282685512367, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4502049, Length=287, Percent_Identity=24.390243902439, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI87082198, Length=251, Percent_Identity=26.6932270916335, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17564128, Length=303, Percent_Identity=28.0528052805281, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71998625, Length=250, Percent_Identity=28.4, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17562292, Length=261, Percent_Identity=26.0536398467433, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17537079, Length=305, Percent_Identity=25.2459016393443, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17561298, Length=257, Percent_Identity=27.6264591439689, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17566692, Length=296, Percent_Identity=23.3108108108108, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17538386, Length=265, Percent_Identity=25.2830188679245, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17561300, Length=261, Percent_Identity=24.1379310344828, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17537077, Length=257, Percent_Identity=25.6809338521401, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17552492, Length=274, Percent_Identity=23.3576642335766, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17537075, Length=257, Percent_Identity=24.124513618677, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17550248, Length=293, Percent_Identity=21.8430034129693, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320079, Length=259, Percent_Identity=29.7297297297297, Blast_Score=89, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6322556, Length=268, Percent_Identity=24.2537313432836, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324694, Length=296, Percent_Identity=26.0135135135135, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320576, Length=273, Percent_Identity=26.3736263736264, Blast_Score=79, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24657054, Length=285, Percent_Identity=27.3684210526316, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24662789, Length=299, Percent_Identity=26.7558528428094, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=24.1258741258741, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24663317, Length=281, Percent_Identity=24.5551601423488, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356425, Length=261, Percent_Identity=24.904214559387, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45553081, Length=286, Percent_Identity=27.6223776223776, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24644950, Length=298, Percent_Identity=21.4765100671141, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281366140, Length=260, Percent_Identity=27.3076923076923, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 32340; Mature: 32340

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYYEEGVGYALQQYYKKSG
CCCCCCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ILRDDIFLQSKFTYTHGQDHRKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYY
CCHHHHEEEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCHHHHHHHEECCCEE
QYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLVELISFASVKPKFVQNRCFARLL
EEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
WDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLSKKHEKSIEQIIFRFSQQIGMLP
HHHHHHHHHHHCCHHHHCHHHEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP
CCCCCCHHHHHHHHHHHHEECCCHHHHHHHHHCCCC
>Mature Secondary Structure
MDLGFPTFIYGTAWKEKRTSELVELALTTGFQGIDTANQRKHYYEEGVGYALQQYYKKSG
CCCCCCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ILRDDIFLQSKFTYTHGQDHRKPYDDTASLTIQVQQSFASSLEHLNTDYLNSYILHGPYY
CCHHHHEEEEEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCHHHHHHHEECCCEE
QYHFSDEDKEVWNKMESLHKDGKVKHLGISNVSYEQLVELISFASVKPKFVQNRCFARLL
EEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
WDKQIRNYCQAHGIIYQGFSLLTANQYELRHPKLENLSKKHEKSIEQIIFRFSQQIGMLP
HHHHHHHHHHHCCHHHHCHHHEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
LTGTTNLDHMKEDLAIHQFRLTDDEINFIENIAFIP
CCCCCCHHHHHHHHHHHHEECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]