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The map label for this gene is ybfF [C]

Identifier: 52840713

GI number: 52840713

Start: 510663

End: 511514

Strand: Reverse

Name: ybfF [C]

Synonym: lpg0468

Alternate gene names: 52840713

Gene position: 511514-510663 (Counterclockwise)

Preceding gene: 52840715

Following gene: 52840710

Centisome position: 15.05

GC content: 38.62

Gene sequence:

>852_bases
TTGATGAAAACAAAGATTTTAAGTATCCCGGGACTATCTATAGCATGCAAAGTATGGGGAAATCCTGATAATCCGCCTAT
TCTTGCCTTGCACGGTTGGTTAGATAATGCTAATAGTTTTGATAATATTGCTGAACATTTACAGAATGATTACTATTTTA
TAGCCGTAGATCTACCTGGACATGGTCATTCTTCACACTTACCCCCAGGATGTATATATCATTTTACTGATGGAATATTT
ACTGTTGTTGAAATTATTAATGCCTTGGGACTAAATAAGCTTCACTTGCTAGGGCACTCTATGGGAGCCTGCTTGGGAAG
TCTTGTAGCTGGGGTTGCGCCGGATCGTTTTCTTTCATTAAGCTTAATCGAAGGTTTAGGCCCTTTTTCTCACCCGGCAG
AAACAGCATGCCAGCAACTGTCAAAATACCTGGACTATCTATCACAAAAACAATCAAAAAAGGCAAAAGGCTACAATAAG
TTTGAACATGCTGCTCTTGCCCGATCCGTTAAAGGATATGTTTCTCTGGATATTGCCAAGTCACTTTGCGAACGCGGAAT
TCAACAAGAAAATGGTCTATATTATTGGAGACACGATCGCAGGCTATTAGCCCCATCTCCATTACAAATGACTGAAGCAC
AAGTTCTTTCCTGCCTTACAGAAATAAAAGCTCAAACTTACCTTATTTGGGCCAGCAAAGGATTTTCATTTGATTCGGAT
AAGATGAAAGCAAGGATTCAAGCAGTAAAAAATATTAAAATTGAGAGGTTAGATGGTGGACACCATATTCATATGGAAAA
GCCAGAAGTCATCAGTCGACTTCTGGCTAAATTTTATCAATCAATAAAATGA

Upstream 100 bases:

>100_bases
AGATTATTTTTTTCGCAAGTCCTGACTATTTTAAAAACTGAATTTACATCCCTGCGTATAAAGTAATAAGCTATAACTAT
TACCTACAAACCAAAGAAAA

Downstream 100 bases:

>100_bases
ATTAATCGACATAACAAGATTGATAATTTATAGTCACTTCACTCAATGACTTCTTAACATCATCCAGATTGTAACCCAGG
TCTTTGGCAGCACTCAACAT

Product: lipase A

Products: NA

Alternate protein names: Atropine acylhydrolase; Atropinesterase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MMKTKILSIPGLSIACKVWGNPDNPPILALHGWLDNANSFDNIAEHLQNDYYFIAVDLPGHGHSSHLPPGCIYHFTDGIF
TVVEIINALGLNKLHLLGHSMGACLGSLVAGVAPDRFLSLSLIEGLGPFSHPAETACQQLSKYLDYLSQKQSKKAKGYNK
FEHAALARSVKGYVSLDIAKSLCERGIQQENGLYYWRHDRRLLAPSPLQMTEAQVLSCLTEIKAQTYLIWASKGFSFDSD
KMKARIQAVKNIKIERLDGGHHIHMEKPEVISRLLAKFYQSIK

Sequences:

>Translated_283_residues
MMKTKILSIPGLSIACKVWGNPDNPPILALHGWLDNANSFDNIAEHLQNDYYFIAVDLPGHGHSSHLPPGCIYHFTDGIF
TVVEIINALGLNKLHLLGHSMGACLGSLVAGVAPDRFLSLSLIEGLGPFSHPAETACQQLSKYLDYLSQKQSKKAKGYNK
FEHAALARSVKGYVSLDIAKSLCERGIQQENGLYYWRHDRRLLAPSPLQMTEAQVLSCLTEIKAQTYLIWASKGFSFDSD
KMKARIQAVKNIKIERLDGGHHIHMEKPEVISRLLAKFYQSIK
>Mature_283_residues
MMKTKILSIPGLSIACKVWGNPDNPPILALHGWLDNANSFDNIAEHLQNDYYFIAVDLPGHGHSSHLPPGCIYHFTDGIF
TVVEIINALGLNKLHLLGHSMGACLGSLVAGVAPDRFLSLSLIEGLGPFSHPAETACQQLSKYLDYLSQKQSKKAKGYNK
FEHAALARSVKGYVSLDIAKSLCERGIQQENGLYYWRHDRRLLAPSPLQMTEAQVLSCLTEIKAQTYLIWASKGFSFDSD
KMKARIQAVKNIKIERLDGGHHIHMEKPEVISRLLAKFYQSIK

Specific function: Alkaloid degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI18777795, Length=295, Percent_Identity=26.1016949152542, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137390, Length=287, Percent_Identity=31.0104529616725, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21357291, Length=287, Percent_Identity=27.8745644599303, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24667992, Length=287, Percent_Identity=27.8745644599303, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24667999, Length=285, Percent_Identity=24.9122807017544, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24655874, Length=282, Percent_Identity=25.531914893617, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24655886, Length=296, Percent_Identity=25.3378378378378, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356953, Length=286, Percent_Identity=26.2237762237762, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356937, Length=288, Percent_Identity=23.9583333333333, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.10 [H]

Molecular weight: Translated: 31525; Mature: 31525

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKTKILSIPGLSIACKVWGNPDNPPILALHGWLDNANSFDNIAEHLQNDYYFIAVDLPG
CCCCEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEEECCC
HGHSSHLPPGCIYHFTDGIFTVVEIINALGLNKLHLLGHSMGACLGSLVAGVAPDRFLSL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SLIEGLGPFSHPAETACQQLSKYLDYLSQKQSKKAKGYNKFEHAALARSVKGYVSLDIAK
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SLCERGIQQENGLYYWRHDRRLLAPSPLQMTEAQVLSCLTEIKAQTYLIWASKGFSFDSD
HHHHCCCCCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHH
KMKARIQAVKNIKIERLDGGHHIHMEKPEVISRLLAKFYQSIK
HHHHHHHHHHCCEEEEECCCCEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKTKILSIPGLSIACKVWGNPDNPPILALHGWLDNANSFDNIAEHLQNDYYFIAVDLPG
CCCCEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEEEEEECCC
HGHSSHLPPGCIYHFTDGIFTVVEIINALGLNKLHLLGHSMGACLGSLVAGVAPDRFLSL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SLIEGLGPFSHPAETACQQLSKYLDYLSQKQSKKAKGYNKFEHAALARSVKGYVSLDIAK
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SLCERGIQQENGLYYWRHDRRLLAPSPLQMTEAQVLSCLTEIKAQTYLIWASKGFSFDSD
HHHHCCCCCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHH
KMKARIQAVKNIKIERLDGGHHIHMEKPEVISRLLAKFYQSIK
HHHHHHHHHHCCEEEEECCCCEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA