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The map label for this gene is ogt [H]

Identifier: 52840639

GI number: 52840639

Start: 436108

End: 436563

Strand: Reverse

Name: ogt [H]

Synonym: lpg0394

Alternate gene names: 52840639

Gene position: 436563-436108 (Counterclockwise)

Preceding gene: 52840640

Following gene: 52840637

Centisome position: 12.85

GC content: 43.2

Gene sequence:

>456_bases
ATGCTCCTCATGTCTATTTTTAATACACCTGTGGGTAACTTGGAAGTAGCCCATGATGAGCATTTTATTTTCAATGCGTC
CTTCACAGACAAATCTGGCGCTCCAGCCCAAAACCAATTGGCCCATTTAATTGCCAGTGAATTGAAGGCTTACTTCGACA
ATCCCCATCACCGTTTTCAGCTCCCTCTGAAACCTCAGGGAAGCCTCTTCCAGCAAAAGGTTTGGAATACGTTGTTAGTC
ATTCCCGTTGGCAGAACAGTTACCTATGGAGAACTCGCTACAAAACTGCAAAGTAGTCCAAGAGCAGTTGGACAAGCCTG
TAAATGCAACCCACTGGCTTTATTTGTTCCTTGTCATAGAGTAGTAGGGAAGCATGATCTTGGAGGATACATGGGAAATC
CAAACGCCATTTCTTATAAAAAAAATCTGCTCGCTCATGAAGCAAAAATCATCTGA

Upstream 100 bases:

>100_bases
TCGTGCCAAATTATATTACTTAAGAAATCTTGCCGGTCGCGCTGCTCGTATTAAAGAAAAATTATCTGGGAAGAAAGGCG
ATTAATTCTTGAATGGTAAA

Downstream 100 bases:

>100_bases
TGAATTAAGTTTATAAAAATCGATATTACATAGATCAGAACTTACAAAAGCACCAAGGCAAAAAATATTCTTAACGAAGA
GATTAGCAAGTCGTGTGAGA

Product: methylated DNA protein cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MLLMSIFNTPVGNLEVAHDEHFIFNASFTDKSGAPAQNQLAHLIASELKAYFDNPHHRFQLPLKPQGSLFQQKVWNTLLV
IPVGRTVTYGELATKLQSSPRAVGQACKCNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII

Sequences:

>Translated_151_residues
MLLMSIFNTPVGNLEVAHDEHFIFNASFTDKSGAPAQNQLAHLIASELKAYFDNPHHRFQLPLKPQGSLFQQKVWNTLLV
IPVGRTVTYGELATKLQSSPRAVGQACKCNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII
>Mature_151_residues
MLLMSIFNTPVGNLEVAHDEHFIFNASFTDKSGAPAQNQLAHLIASELKAYFDNPHHRFQLPLKPQGSLFQQKVWNTLLV
IPVGRTVTYGELATKLQSSPRAVGQACKCNPLALFVPCHRVVGKHDLGGYMGNPNAISYKKNLLAHEAKII

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=169, Percent_Identity=33.1360946745562, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI1788542, Length=106, Percent_Identity=41.5094339622642, Blast_Score=76, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=67, Percent_Identity=50.7462686567164, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16674; Mature: 16674

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLMSIFNTPVGNLEVAHDEHFIFNASFTDKSGAPAQNQLAHLIASELKAYFDNPHHRFQ
CEEEEECCCCCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
LPLKPQGSLFQQKVWNTLLVIPVGRTVTYGELATKLQSSPRAVGQACKCNPLALFVPCHR
CCCCCCCHHHHHHHCCEEEEEECCCEEEHHHHHHHHHCCCHHHHHCCCCCCEEEEEEHHH
VVGKHDLGGYMGNPNAISYKKNLLAHEAKII
HHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLLMSIFNTPVGNLEVAHDEHFIFNASFTDKSGAPAQNQLAHLIASELKAYFDNPHHRFQ
CEEEEECCCCCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
LPLKPQGSLFQQKVWNTLLVIPVGRTVTYGELATKLQSSPRAVGQACKCNPLALFVPCHR
CCCCCCCHHHHHHHCCEEEEEECCCEEEHHHHHHHHHCCCHHHHHCCCCCCEEEEEEHHH
VVGKHDLGGYMGNPNAISYKKNLLAHEAKII
HHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]