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The map label for this gene is fixL

Identifier: 52840533

GI number: 52840533

Start: 330852

End: 332267

Strand: Reverse

Name: fixL

Synonym: lpg0278

Alternate gene names: 52840533

Gene position: 332267-330852 (Counterclockwise)

Preceding gene: 52840534

Following gene: 52840532

Centisome position: 9.78

GC content: 35.66

Gene sequence:

>1416_bases
ATGACAGAAATGCATCGGTTGTTGCAGCGACAGTTAAAAAAATTTGGTTTTACTGCTGATGGATTACCACAAAATCTGGA
GAGTTGGACTCAATTTATTGCACATATCAATAAAACATACTACGAAAACGATCAAGACCGATATTTATTAGAGCGCTCTA
TTGATATTTCTTCTAAAGAAATGAGGGATATCAATGCAAAACTCGAATACGCCCAGGATATAGCGCAAATTGGGTATTGG
CATTATGACGCTGACACAGATACCGCAACATGGTCCAAAGGACTATTTAAAATTCTGGGTTTGCCGCCTAGTAATGTGGC
ACTAAACTATTCTTCATTTTTAGATCTTGTGCATCCTAATGATAAAGCAGGGTTAAAAAATGTGGTCGAAAAGGCCCTCA
AAGAAAAAGAAGATTATGATTATGAGCTTAGAGTAAGAAACGGAGACAATGATTACAAATGGTACCGAACGATAGCAAGA
GCCAATCCTCATAAAAACGAAATTTCCGGGGTTTTGATGGATATCTCCAAAAGGAAAATTATTGAAGAGCAAGTTCATGA
ACTGAATAATAAACTGGTTTCAACAGCTCGACTTGCCGGGATGTCGGAAGTAGCCACCTCGATACTTCATAATGTGGGCA
ATATATTAAACAGCGTCAATGTTTCTGCCATTATTCTGAAAAAGAATATATCCAAAATTCATTATAAAAAACTGTCCGCT
ATAAAAACCATGCTTGACGAAAACATCAATCAATTAAGCGAATACTTGTTACAGAATCCAAAGGGTAAGTTAATTCTTCC
CTATTTGGACGCGTTATCGAAAACCCTGCATGAAGAATATGAAGAAAACATGAAGGAATCCAACAGTCTGGAAACTAACA
TTAATCATATTAAAGAAATCATAGCCATGCAGCAATCATTGAGCGGCTTATCGAATTTAACAGAGAAAATTTCAATCCAT
GATGTGATTCTTAATGCGGTAAAATCAGTCATATTGGACAAACATCAAATTGATGTCCGTATTGAGATTGAACCGGATAT
CAATGAGTTTATCACTGATAAATCCAAGTTATTTCAAATTCTTACTAATTTACTCGCTAATGCAAAAGATTCTGTTTTAT
TACACGAAGCGAATGATTCCAGGAAAGTTGTTATTTCCATTGGGAGAACTGAAAAAAATCATATTATGATTAAAATAAAT
GATAATGGAATTGGAATTCAAAAAGATAACCTGGATCGAATTTTTTCTTTTGGATTTACAACCAAGAAAAATGGTCACGG
CTTTGGACTGCACTGTTCTGCAATTTATGCACGGGATCTGGGTGGTACACTTTATGCTGAAAGTAATGGCAATGGCAAAG
GAGCTCAATTTGTATTAGATTTGCCCATCAATCCCAAAACCTTGAGTACGAGGTAG

Upstream 100 bases:

>100_bases
TTATCACCTCTGGCAACAGGTACCTGCGAATTGCATAATCAAACCATGACCATTACGACCCTGTTAGAGAGTCCATAAAC
CAAAAAGGAATTCAGTAATA

Downstream 100 bases:

>100_bases
AAATGAGTACTGAATCTTTTCGCATTCTGGTCATTGATGACAACCCGGCAATCCATCAGGATTTTATGAAAATCTTAAAT
GTCAGCAAGAACTCTGTATT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYENDQDRYLLERSIDISSKEMRDINAKLEYAQDIAQIGYW
HYDADTDTATWSKGLFKILGLPPSNVALNYSSFLDLVHPNDKAGLKNVVEKALKEKEDYDYELRVRNGDNDYKWYRTIAR
ANPHKNEISGVLMDISKRKIIEEQVHELNNKLVSTARLAGMSEVATSILHNVGNILNSVNVSAIILKKNISKIHYKKLSA
IKTMLDENINQLSEYLLQNPKGKLILPYLDALSKTLHEEYEENMKESNSLETNINHIKEIIAMQQSLSGLSNLTEKISIH
DVILNAVKSVILDKHQIDVRIEIEPDINEFITDKSKLFQILTNLLANAKDSVLLHEANDSRKVVISIGRTEKNHIMIKIN
DNGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGGTLYAESNGNGKGAQFVLDLPINPKTLSTR

Sequences:

>Translated_471_residues
MTEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYENDQDRYLLERSIDISSKEMRDINAKLEYAQDIAQIGYW
HYDADTDTATWSKGLFKILGLPPSNVALNYSSFLDLVHPNDKAGLKNVVEKALKEKEDYDYELRVRNGDNDYKWYRTIAR
ANPHKNEISGVLMDISKRKIIEEQVHELNNKLVSTARLAGMSEVATSILHNVGNILNSVNVSAIILKKNISKIHYKKLSA
IKTMLDENINQLSEYLLQNPKGKLILPYLDALSKTLHEEYEENMKESNSLETNINHIKEIIAMQQSLSGLSNLTEKISIH
DVILNAVKSVILDKHQIDVRIEIEPDINEFITDKSKLFQILTNLLANAKDSVLLHEANDSRKVVISIGRTEKNHIMIKIN
DNGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGGTLYAESNGNGKGAQFVLDLPINPKTLSTR
>Mature_470_residues
TEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYENDQDRYLLERSIDISSKEMRDINAKLEYAQDIAQIGYWH
YDADTDTATWSKGLFKILGLPPSNVALNYSSFLDLVHPNDKAGLKNVVEKALKEKEDYDYELRVRNGDNDYKWYRTIARA
NPHKNEISGVLMDISKRKIIEEQVHELNNKLVSTARLAGMSEVATSILHNVGNILNSVNVSAIILKKNISKIHYKKLSAI
KTMLDENINQLSEYLLQNPKGKLILPYLDALSKTLHEEYEENMKESNSLETNINHIKEIIAMQQSLSGLSNLTEKISIHD
VILNAVKSVILDKHQIDVRIEIEPDINEFITDKSKLFQILTNLLANAKDSVLLHEANDSRKVVISIGRTEKNHIMIKIND
NGIGIQKDNLDRIFSFGFTTKKNGHGFGLHCSAIYARDLGGTLYAESNGNGKGAQFVLDLPINPKTLSTR

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=133, Percent_Identity=34.5864661654135, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI48994928, Length=138, Percent_Identity=31.1594202898551, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53520; Mature: 53389

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYENDQDRYLLERSIDISSKE
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHH
MRDINAKLEYAQDIAQIGYWHYDADTDTATWSKGLFKILGLPPSNVALNYSSFLDLVHPN
HHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCCCCCEEEHHHHHHCCCCC
DKAGLKNVVEKALKEKEDYDYELRVRNGDNDYKWYRTIARANPHKNEISGVLMDISKRKI
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IEEQVHELNNKLVSTARLAGMSEVATSILHNVGNILNSVNVSAIILKKNISKIHYKKLSA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHH
IKTMLDENINQLSEYLLQNPKGKLILPYLDALSKTLHEEYEENMKESNSLETNINHIKEI
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IAMQQSLSGLSNLTEKISIHDVILNAVKSVILDKHQIDVRIEIEPDINEFITDKSKLFQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHCCHHHHHHH
LTNLLANAKDSVLLHEANDSRKVVISIGRTEKNHIMIKINDNGIGIQKDNLDRIFSFGFT
HHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCC
TKKNGHGFGLHCSAIYARDLGGTLYAESNGNGKGAQFVLDLPINPKTLSTR
CCCCCCCCCEEEHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TEMHRLLQRQLKKFGFTADGLPQNLESWTQFIAHINKTYYENDQDRYLLERSIDISSKE
CHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHH
MRDINAKLEYAQDIAQIGYWHYDADTDTATWSKGLFKILGLPPSNVALNYSSFLDLVHPN
HHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCCCCCEEEHHHHHHCCCCC
DKAGLKNVVEKALKEKEDYDYELRVRNGDNDYKWYRTIARANPHKNEISGVLMDISKRKI
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
IEEQVHELNNKLVSTARLAGMSEVATSILHNVGNILNSVNVSAIILKKNISKIHYKKLSA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHH
IKTMLDENINQLSEYLLQNPKGKLILPYLDALSKTLHEEYEENMKESNSLETNINHIKEI
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IAMQQSLSGLSNLTEKISIHDVILNAVKSVILDKHQIDVRIEIEPDINEFITDKSKLFQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHCCHHHHHHH
LTNLLANAKDSVLLHEANDSRKVVISIGRTEKNHIMIKINDNGIGIQKDNLDRIFSFGFT
HHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCC
TKKNGHGFGLHCSAIYARDLGGTLYAESNGNGKGAQFVLDLPINPKTLSTR
CCCCCCCCCEEEHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]