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The map label for this gene is mdlC

Identifier: 52840488

GI number: 52840488

Start: 270686

End: 272278

Strand: Reverse

Name: mdlC

Synonym: lpg0233

Alternate gene names: 52840488

Gene position: 272278-270686 (Counterclockwise)

Preceding gene: 52840489

Following gene: 52840486

Centisome position: 8.01

GC content: 39.49

Gene sequence:

>1593_bases
GTGAAAAAAACAGGAAGTGATGTTCTCAAAGAATTTATAACGACCTTTGAAATTAATTATATTTTTGGCAACCCCGGCAC
TACAGAATTGAAATTTCTGGAAGCGATTGAGCAGTGTGATAATGCCACCTATTTTCTCACACTACAAGAATCCAGTACCG
TAGGTATAGCCGCAGGCTATGCCATGATCACCAGGAAACCAGCTCTTATTAATTTACATAATTATGTTGGCTTAGCGAAT
GCTGTCTGTAATATGTACAATGCATTCACTTCAGGAATTCCCCTGCTAATAATTGCTGGCCAACATGCACGTCCTTATTT
AATTCATAGCCCAGTCATGTCCGGAGACTTGACCTCTTTAGCTCACACTGCAGTAAAATATGCCTATGAGGTGAATTCCG
CCAGCGATCTGGCCGTTGCTTTACAGCGTGGTTATGCTCAGGCAATTTTGCCTGGGCCAGGTCCGGTTTTTCTATCAATC
CCTATGGACATTTGGCAAGAAGAAACTCAAGAAACCACGATAAAGAAGACAAAATTTTTACATGCTTGCATCAACGCTGA
AATAGAGCAAATTTGTGAGGTTTTAACAAATACCCCCAAAGGAAAATTGGCATTTATAGCGGATTATGAGGTAGGTGCAT
CGAATAGTATTCGCCTTTTACATACCATTGCCGATCGGCTAAATGCCGATATTTATGCTGCCCCCTATCATGTGCGTGAA
GTAATTAATCCCTTAAGTGTAAATTACAAAGGTAGATTGTCAGATAAATCTGGAGAAGTGCACAACCTGCTGCTGCAATA
TGAAACTGTGGTACTTTTAGGCGAAAAAGTGAAAGGATATTTGTATACTGGTCAATCTTCTATTCCACAGGGTATACGAT
TCATACAAATCAGTGCCGCATCGGAGCATCTGGCCTTTGACTATCCTTGCGATCTGGCAATTCTTGGCAATCTTGAGGCG
ACCTTAACTGCTATTAGCAAGCATTTAAAAATTAATGGGCTAAATGTGCCGCTTAAACCAGCCACCAACATTAAAAATTT
GGAAAAAAAATATAAGCAACATCCTCTGCATTCGTTGATTGTTACCCTCCTGAATCAAGTAAATCGCTCTGTTCATTTAA
TAACAGAAGGATCATCAGAAGATGCTATTGTGCAGGACATTGCAACAACACTAGGTTTTTTAAATGTGAATTTTTCACCA
AGAGGGGGTGGGCTTGGCTGGGCTATGCCATTAGCAATAGGTATTTCTCTAGCGACCAGACACCATTCAATTTGTTTTGT
AGGAGATGGGGGCAGCATGTATGCGATTCATTCAATATGGTCAGCTGCCAAATATAAGATTCCTGTCATTTTCATTTGTT
TTGTTAATCGAGAATACAGAGTATTAAAAACAGATATGTCTCAAAATAGATCTTTCACAGAAGAAGATTTTTTTCGCCTG
GATTTGAATCATCCACCAATTGATGTTCAACAAATTGCCTTATCTTTCGGGGCACAGGTGAAGACCATTAAATCAAAAGA
AGAAGTAGAGACTGTTCTCAAGAGTAGCTTTGCATTCCAAGGCCCAACATTTATTACCCTATATCATCAATAA

Upstream 100 bases:

>100_bases
TTTGCCCTATTTTTTATGAGTGAGCGAAATTGAACAAATAGCTCTCTTGTGCTAGGAATATAAATATGGAAAGCGTTACT
ATTTCTAAGGATGCTTATCT

Downstream 100 bases:

>100_bases
AACATTTCGAAAGCCAGACATTGGCTTTTGCATTATCTAAAAATGTAAAGCTTAAAATCAGTCTCATTATTTAAAAATTT
TTATTTTACAACTCATAGGA

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATYFLTLQESSTVGIAAGYAMITRKPALINLHNYVGLAN
AVCNMYNAFTSGIPLLIIAGQHARPYLIHSPVMSGDLTSLAHTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSI
PMDIWQEETQETTIKKTKFLHACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIRLLHTIADRLNADIYAAPYHVRE
VINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEKVKGYLYTGQSSIPQGIRFIQISAASEHLAFDYPCDLAILGNLEA
TLTAISKHLKINGLNVPLKPATNIKNLEKKYKQHPLHSLIVTLLNQVNRSVHLITEGSSEDAIVQDIATTLGFLNVNFSP
RGGGLGWAMPLAIGISLATRHHSICFVGDGGSMYAIHSIWSAAKYKIPVIFICFVNREYRVLKTDMSQNRSFTEEDFFRL
DLNHPPIDVQQIALSFGAQVKTIKSKEEVETVLKSSFAFQGPTFITLYHQ

Sequences:

>Translated_530_residues
MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATYFLTLQESSTVGIAAGYAMITRKPALINLHNYVGLAN
AVCNMYNAFTSGIPLLIIAGQHARPYLIHSPVMSGDLTSLAHTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSI
PMDIWQEETQETTIKKTKFLHACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIRLLHTIADRLNADIYAAPYHVRE
VINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEKVKGYLYTGQSSIPQGIRFIQISAASEHLAFDYPCDLAILGNLEA
TLTAISKHLKINGLNVPLKPATNIKNLEKKYKQHPLHSLIVTLLNQVNRSVHLITEGSSEDAIVQDIATTLGFLNVNFSP
RGGGLGWAMPLAIGISLATRHHSICFVGDGGSMYAIHSIWSAAKYKIPVIFICFVNREYRVLKTDMSQNRSFTEEDFFRL
DLNHPPIDVQQIALSFGAQVKTIKSKEEVETVLKSSFAFQGPTFITLYHQ
>Mature_530_residues
MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATYFLTLQESSTVGIAAGYAMITRKPALINLHNYVGLAN
AVCNMYNAFTSGIPLLIIAGQHARPYLIHSPVMSGDLTSLAHTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSI
PMDIWQEETQETTIKKTKFLHACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIRLLHTIADRLNADIYAAPYHVRE
VINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEKVKGYLYTGQSSIPQGIRFIQISAASEHLAFDYPCDLAILGNLEA
TLTAISKHLKINGLNVPLKPATNIKNLEKKYKQHPLHSLIVTLLNQVNRSVHLITEGSSEDAIVQDIATTLGFLNVNFSP
RGGGLGWAMPLAIGISLATRHHSICFVGDGGSMYAIHSIWSAAKYKIPVIFICFVNREYRVLKTDMSQNRSFTEEDFFRL
DLNHPPIDVQQIALSFGAQVKTIKSKEEVETVLKSSFAFQGPTFITLYHQ

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=522, Percent_Identity=20.1149425287356, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788716, Length=503, Percent_Identity=21.6699801192843, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=234, Percent_Identity=23.5042735042735, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 58498; Mature: 58498

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATYFLTLQESSTVGIAAGY
CCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEEECC
AMITRKPALINLHNYVGLANAVCNMYNAFTSGIPLLIIAGQHARPYLIHSPVMSGDLTSL
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCHHHH
AHTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSIPMDIWQEETQETTIKKTKFL
HHHHHHHEEECCCCHHHHHHHHCCCCEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
HACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIRLLHTIADRLNADIYAAPYHVRE
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEECCHHHHH
VINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEKVKGYLYTGQSSIPQGIRFIQISAA
HHCCCCCCEEEECCCCCCHHHHHHHHHEEEEEEECCCCEEEEECCCCCCCCEEEEEEEEC
SEHLAFDYPCDLAILGNLEATLTAISKHLKINGLNVPLKPATNIKNLEKKYKQHPLHSLI
CCCEEECCCCCEEEECCCHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHH
VTLLNQVNRSVHLITEGSSEDAIVQDIATTLGFLNVNFSPRGGGLGWAMPLAIGISLATR
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHEEEC
HHSICFVGDGGSMYAIHSIWSAAKYKIPVIFICFVNREYRVLKTDMSQNRSFTEEDFFRL
CCEEEEEECCCCEEEHHHHHHCCCCCCCEEEEEEECCCEEEEECCHHCCCCCCCCCEEEE
DLNHPPIDVQQIALSFGAQVKTIKSKEEVETVLKSSFAFQGPTFITLYHQ
ECCCCCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MKKTGSDVLKEFITTFEINYIFGNPGTTELKFLEAIEQCDNATYFLTLQESSTVGIAAGY
CCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEEECC
AMITRKPALINLHNYVGLANAVCNMYNAFTSGIPLLIIAGQHARPYLIHSPVMSGDLTSL
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCHHHH
AHTAVKYAYEVNSASDLAVALQRGYAQAILPGPGPVFLSIPMDIWQEETQETTIKKTKFL
HHHHHHHEEECCCCHHHHHHHHCCCCEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
HACINAEIEQICEVLTNTPKGKLAFIADYEVGASNSIRLLHTIADRLNADIYAAPYHVRE
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEECCHHHHH
VINPLSVNYKGRLSDKSGEVHNLLLQYETVVLLGEKVKGYLYTGQSSIPQGIRFIQISAA
HHCCCCCCEEEECCCCCCHHHHHHHHHEEEEEEECCCCEEEEECCCCCCCCEEEEEEEEC
SEHLAFDYPCDLAILGNLEATLTAISKHLKINGLNVPLKPATNIKNLEKKYKQHPLHSLI
CCCEEECCCCCEEEECCCHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHH
VTLLNQVNRSVHLITEGSSEDAIVQDIATTLGFLNVNFSPRGGGLGWAMPLAIGISLATR
HHHHHHCCCEEEEEECCCCCCHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHEEEC
HHSICFVGDGGSMYAIHSIWSAAKYKIPVIFICFVNREYRVLKTDMSQNRSFTEEDFFRL
CCEEEEEECCCCEEEHHHHHHCCCCCCCEEEEEEECCCEEEEECCHHCCCCCCCCCEEEE
DLNHPPIDVQQIALSFGAQVKTIKSKEEVETVLKSSFAFQGPTFITLYHQ
ECCCCCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]