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The map label for this gene is prpB

Identifier: 52840308

GI number: 52840308

Start: 60077

End: 60970

Strand: Reverse

Name: prpB

Synonym: lpg0052

Alternate gene names: 52840308

Gene position: 60970-60077 (Counterclockwise)

Preceding gene: 52840309

Following gene: 52840307

Centisome position: 1.79

GC content: 42.39

Gene sequence:

>894_bases
ATGTCAAATTTTCGATACAGCCAGGGAAAAAAACTACGCGAAGCGTTAATGAATGAAAAACCATTGCAAGTTGTAGGAAC
AATTAATGCCTATACCGCTTTGCAAGCCAAAAGAGCAGGATTTCATGCAATTTATCTTTCAGGAGCAGGAGTAGCTAACG
CCTCTTACGGCTTACCAGACTTGGGCATTACCACATTAAATGATGTACTCGAAGACGTACGGCGCATTATGAGTGCCGTT
GATTTACCCCTCCTGGTCGATATTGATACAGGATGGGGTGGAGCCTTTAGTATCGCACGGACTATCAAAGAAATGATTAA
AGCTGAGGCAGCAGCGGTACATATTGAAGATCAGGTTCAAGCGAAACGATGTGGTCATCGCCCGGGTAAGGCATTGGTTG
AAAAAGAAGAAATGATAGATCGTATTAAGGCCGCTGTGGATGCGAAAACTGATCCTGATTTTGTTATTATGGCAAGAACT
GATGCCCTGGCGAATGAAGGATTAAATAAGGCGCTGGAGCGAATTAGTGCTTATATTGAAGCCGGGGCAGATATGATTTT
CTTCGAAGGAGTACGTAAACTTGAGGAATATCAGGCGCTAACTGAGCAATGTAATGTTCCGGTATTGGCCAATATCACTG
AATTTGGAGTTACTCCATTATTTACGCTGGAAGAATTAGAGGAAGTAGGAGTTAGCCTGGCTCTTTATCCTTTAAGTGCA
TTTCGGGCAATGAGTGCCGCTGCAGAAAAAGTTTACGACACAATCCGCAAAAACGGCAGCCAAAACGATATTCTGGCAGA
GATGCAAACTCGCGAAGAGTTGTATCAGGTATTAAATTACCATTTTTACGAAGACAAGCTTAATGAACTCTTTATGAAAG
AAAAAACGACGTGA

Upstream 100 bases:

>100_bases
ATAAACTAAATGACCGAATTAAAAACTTTTTTTAACAAAGAGATTGGGATTTTTCGTAAGTCCTGATAAATTCTCATCTT
AGGCAAAACAAGGAAACCAA

Downstream 100 bases:

>100_bases
ATATCAATTAATTATCAGAACTAATCTGAGAAAATAAGGTTTTATATGCCCGGATAGAGGATTATCTGCCGAAATTGATG
TTTATAAACAACAGATTAAC

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSNFRYSQGKKLREALMNEKPLQVVGTINAYTALQAKRAGFHAIYLSGAGVANASYGLPDLGITTLNDVLEDVRRIMSAV
DLPLLVDIDTGWGGAFSIARTIKEMIKAEAAAVHIEDQVQAKRCGHRPGKALVEKEEMIDRIKAAVDAKTDPDFVIMART
DALANEGLNKALERISAYIEAGADMIFFEGVRKLEEYQALTEQCNVPVLANITEFGVTPLFTLEELEEVGVSLALYPLSA
FRAMSAAAEKVYDTIRKNGSQNDILAEMQTREELYQVLNYHFYEDKLNELFMKEKTT

Sequences:

>Translated_297_residues
MSNFRYSQGKKLREALMNEKPLQVVGTINAYTALQAKRAGFHAIYLSGAGVANASYGLPDLGITTLNDVLEDVRRIMSAV
DLPLLVDIDTGWGGAFSIARTIKEMIKAEAAAVHIEDQVQAKRCGHRPGKALVEKEEMIDRIKAAVDAKTDPDFVIMART
DALANEGLNKALERISAYIEAGADMIFFEGVRKLEEYQALTEQCNVPVLANITEFGVTPLFTLEELEEVGVSLALYPLSA
FRAMSAAAEKVYDTIRKNGSQNDILAEMQTREELYQVLNYHFYEDKLNELFMKEKTT
>Mature_296_residues
SNFRYSQGKKLREALMNEKPLQVVGTINAYTALQAKRAGFHAIYLSGAGVANASYGLPDLGITTLNDVLEDVRRIMSAVD
LPLLVDIDTGWGGAFSIARTIKEMIKAEAAAVHIEDQVQAKRCGHRPGKALVEKEEMIDRIKAAVDAKTDPDFVIMARTD
ALANEGLNKALERISAYIEAGADMIFFEGVRKLEEYQALTEQCNVPVLANITEFGVTPLFTLEELEEVGVSLALYPLSAF
RAMSAAAEKVYDTIRKNGSQNDILAEMQTREELYQVLNYHFYEDKLNELFMKEKTT

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=291, Percent_Identity=62.8865979381443, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790445, Length=168, Percent_Identity=35.7142857142857, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71982926, Length=221, Percent_Identity=31.6742081447964, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17561814, Length=221, Percent_Identity=31.6742081447964, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32980; Mature: 32849

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNFRYSQGKKLREALMNEKPLQVVGTINAYTALQAKRAGFHAIYLSGAGVANASYGLPD
CCCCCCCCCHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
LGITTLNDVLEDVRRIMSAVDLPLLVDIDTGWGGAFSIARTIKEMIKAEAAAVHIEDQVQ
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHH
AKRCGHRPGKALVEKEEMIDRIKAAVDAKTDPDFVIMARTDALANEGLNKALERISAYIE
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
AGADMIFFEGVRKLEEYQALTEQCNVPVLANITEFGVTPLFTLEELEEVGVSLALYPLSA
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCCHHHHHHHHHCCCEEEEHHHHH
FRAMSAAAEKVYDTIRKNGSQNDILAEMQTREELYQVLNYHFYEDKLNELFMKEKTT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNFRYSQGKKLREALMNEKPLQVVGTINAYTALQAKRAGFHAIYLSGAGVANASYGLPD
CCCCCCCCHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
LGITTLNDVLEDVRRIMSAVDLPLLVDIDTGWGGAFSIARTIKEMIKAEAAAVHIEDQVQ
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHH
AKRCGHRPGKALVEKEEMIDRIKAAVDAKTDPDFVIMARTDALANEGLNKALERISAYIE
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
AGADMIFFEGVRKLEEYQALTEQCNVPVLANITEFGVTPLFTLEELEEVGVSLALYPLSA
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCCHHHHHHHHHCCCEEEEHHHHH
FRAMSAAAEKVYDTIRKNGSQNDILAEMQTREELYQVLNYHFYEDKLNELFMKEKTT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]