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The map label for this gene is nudF [H]

Identifier: 39998443

GI number: 39998443

Start: 3684928

End: 3685449

Strand: Reverse

Name: nudF [H]

Synonym: GSU3354

Alternate gene names: 39998443

Gene position: 3685449-3684928 (Counterclockwise)

Preceding gene: 39998447

Following gene: 39998442

Centisome position: 96.63

GC content: 65.33

Gene sequence:

>522_bases
ATGGATACCCGCAACAGGATGATTCTTTTCAACGGCCTGGTGGTTAATGTGGAACAGATGGACGTCCGGATCGGCGCCAA
AGGGTGGCACACGTTTCAGGTTGTGCGGCATCCGGGCGGGGTGGGAGTCCTTCCGCTCCACGAGGACGGAACGGTCACCC
TGATCCGCCAGCTCCGGCCGGCCGTGGACGATATGCTGCTGGAGATCCCGGCGGGTCGGCTCGATCCGGGCGAGGAGCCT
TCCGCCTGCGGCCGGCGCGAATTGACCGAGGAGACGGGACTTACGGCTGAGCGGCTCGAATCCCTCGGCACGATCCTGAC
CTCTCCGGGGGTCTTCGACGAGCGGATCCACCTGTTTCTCGCCGTCGGTCTTACCCAGGGGGAAGCGACCCCCGAGCAGT
ACGAGGAAATCGAAACCGTGCGCCTTCCCCTGGCCGATGCCCTGGCCCTGGCCGCCGAGTGCGGAATTCGGGATGGCAAG
ACCATTGCGGCCCTTCTGCGGGCCTCGGTGCGCCAGTCATGA

Upstream 100 bases:

>100_bases
GCATCCGAAGCGACTCCAGCCTGTGCCCTCCGGCAATAAACCGCTTTTCCCCCGCCGGTGATGGTGGTACTCTCCTCCCA
TTTCGCGGAGGATGGAGTGC

Downstream 100 bases:

>100_bases
TCCTGACGGCGCGGGTGGGGGCCGACCAGGCCGGCGTACGGCTCGACGAGGGGGCGGCGCGGCTCTTTCCCCAGCTCTCC
AAAACCCGGATCCGTACTAT

Product: mutT/nudix family protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MDTRNRMILFNGLVVNVEQMDVRIGAKGWHTFQVVRHPGGVGVLPLHEDGTVTLIRQLRPAVDDMLLEIPAGRLDPGEEP
SACGRRELTEETGLTAERLESLGTILTSPGVFDERIHLFLAVGLTQGEATPEQYEEIETVRLPLADALALAAECGIRDGK
TIAALLRASVRQS

Sequences:

>Translated_173_residues
MDTRNRMILFNGLVVNVEQMDVRIGAKGWHTFQVVRHPGGVGVLPLHEDGTVTLIRQLRPAVDDMLLEIPAGRLDPGEEP
SACGRRELTEETGLTAERLESLGTILTSPGVFDERIHLFLAVGLTQGEATPEQYEEIETVRLPLADALALAAECGIRDGK
TIAALLRASVRQS
>Mature_173_residues
MDTRNRMILFNGLVVNVEQMDVRIGAKGWHTFQVVRHPGGVGVLPLHEDGTVTLIRQLRPAVDDMLLEIPAGRLDPGEEP
SACGRRELTEETGLTAERLESLGTILTSPGVFDERIHLFLAVGLTQGEATPEQYEEIETVRLPLADALALAAECGIRDGK
TIAALLRASVRQS

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788810, Length=150, Percent_Identity=34.6666666666667, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 18846; Mature: 18846

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTRNRMILFNGLVVNVEQMDVRIGAKGWHTFQVVRHPGGVGVLPLHEDGTVTLIRQLRP
CCCCCCEEEECCEEEEEEEEEEEECCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHH
AVDDMLLEIPAGRLDPGEEPSACGRRELTEETGLTAERLESLGTILTSPGVFDERIHLFL
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHEEEEE
AVGLTQGEATPEQYEEIETVRLPLADALALAAECGIRDGKTIAALLRASVRQS
EEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTRNRMILFNGLVVNVEQMDVRIGAKGWHTFQVVRHPGGVGVLPLHEDGTVTLIRQLRP
CCCCCCEEEECCEEEEEEEEEEEECCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHH
AVDDMLLEIPAGRLDPGEEPSACGRRELTEETGLTAERLESLGTILTSPGVFDERIHLFL
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHEEEEE
AVGLTQGEATPEQYEEIETVRLPLADALALAAECGIRDGKTIAALLRASVRQS
EEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]