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The map label for this gene is glcF [H]

Identifier: 39998386

GI number: 39998386

Start: 3616956

End: 3618221

Strand: Direct

Name: glcF [H]

Synonym: GSU3297

Alternate gene names: 39998386

Gene position: 3616956-3618221 (Clockwise)

Preceding gene: 39998385

Following gene: 39998387

Centisome position: 94.83

GC content: 63.74

Gene sequence:

>1266_bases
TTGGATCCGCTGAAACGCGTCGAAAACGAACTCAAAAAATGCGTCAAGTGCGGCGCCTGCCGCGCCCATTGCCCGGCCTT
TGCCGCCTGGCAGCGGGAGCCGGCCGTGGCCCGAGGCAAGGTGGCCATCGCCCAGCACATCCTCAAGGGTGACATCGAGC
TGGACGAGCAGACCTACGCGGTCATGTCCAAGTGCCTGCTCTGCGGCAGTTGCGTGGAAAAGTGCCCCAACGACGTGCCC
ACCGACGAGATCGTCGTCACCGCCCGGGAGTCCCTGGCCCGGCGCCGCGGACTCACCACCTTCCACAAGGCGGTGGGACA
GGTCATTAAAAACCGCCGGCTGATGTCCTTCGGCGCTCTTGCCGCCGCAATCCTGGGGCCGCTCTTTTTCCGCAAGGTCC
CCCAGACCTCGGGGCTGCGCCTCCGCTTCCCCCTTCCCTTCGTGGGCAACCGCCGCCACATCCCCCAGATCGCCCGTAAA
CCCTTCATGGACCGTCACCCGGAAGTTATCCAGGGAGAGCCGGGCAAACCCCGCGTCGTCTTCTATGTCGGTTGCATGAC
CAACTTCGTCTACACCGAGGTGGGTGAAGCGGCCCTGGCCCTTTTCCGGCACCTGGGCTGTACCGTCATCATCCCTAAGG
GGCAGGGCTGTTGCGGTCTCCCGGGCATGTCGGGCGGCGACCTGGATACGGTTCGCGGCCTTGCTGAGCAGAATCTGGCG
GCCCTTGAACGGTACGAGGCCGATTACATCATGACAGCCTGCGCCACCTGCGGCGGGGCGCTCCATAAATTCTACCCCGC
CCTCATCGGCAAACGGCATCCGGAACTGGCAGCGCGAATCAGGACCATTGCCGCCAAAACGGTCGACGCGGCCCAGCTCC
TCCAGCAGCTCGGGCTGCATCCGGAGGAAACGGGGGCGGGCGAACGCCTGAAGATCACGTACCACGACCCCTGCCACCTG
CGGACCAGGAACATCACCCGCCAGCCCCGCGAACTGCTGAAAGGCTCTCCCGGGGTCGAACTCGCGGAGATGGAGGGTGC
CGACAAATGTTGCGGACTTGGCGGCACCTTCAATGTCTATCACTATGACACGTCCCTGAAGATCAATGAGGGAAAGAGCG
CAGCCATCGAAAAGACCGGTGCCGACGCGGTCGTCACCGGTTGTCCCGGCTGTATGATGCAACTCTCCGACGGGCTCAAG
CAGCACGGTAACCGCACCCGGGTCATGCATACCCTTGAAGTGCTCGCCCGAGCGCTCAGAAAGTAA

Upstream 100 bases:

>100_bases
GTTTCCCGCGTGATGCGGCGGCAGCCGCCAGGTACGCCGTGAGCGGCTTGCGGGCACAGATTCAGTTCCGCGCACTGCGC
GACAAAGAAAGGTCGTAGAT

Downstream 100 bases:

>100_bases
CCGCTGCGGGGGAGCGGGACTCTCGTCCCGTTCCCCCGTCCACGCTCCCCCGAAAAAGTCGTTCTAATTCTTACCGCGCC
CCGCGGAAACGCCCTCCCAC

Product: iron-sulfur cluster-binding protein

Products: FADH2; dihydroxy-acetone-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MDPLKRVENELKKCVKCGACRAHCPAFAAWQREPAVARGKVAIAQHILKGDIELDEQTYAVMSKCLLCGSCVEKCPNDVP
TDEIVVTARESLARRRGLTTFHKAVGQVIKNRRLMSFGALAAAILGPLFFRKVPQTSGLRLRFPLPFVGNRRHIPQIARK
PFMDRHPEVIQGEPGKPRVVFYVGCMTNFVYTEVGEAALALFRHLGCTVIIPKGQGCCGLPGMSGGDLDTVRGLAEQNLA
ALERYEADYIMTACATCGGALHKFYPALIGKRHPELAARIRTIAAKTVDAAQLLQQLGLHPEETGAGERLKITYHDPCHL
RTRNITRQPRELLKGSPGVELAEMEGADKCCGLGGTFNVYHYDTSLKINEGKSAAIEKTGADAVVTGCPGCMMQLSDGLK
QHGNRTRVMHTLEVLARALRK

Sequences:

>Translated_421_residues
MDPLKRVENELKKCVKCGACRAHCPAFAAWQREPAVARGKVAIAQHILKGDIELDEQTYAVMSKCLLCGSCVEKCPNDVP
TDEIVVTARESLARRRGLTTFHKAVGQVIKNRRLMSFGALAAAILGPLFFRKVPQTSGLRLRFPLPFVGNRRHIPQIARK
PFMDRHPEVIQGEPGKPRVVFYVGCMTNFVYTEVGEAALALFRHLGCTVIIPKGQGCCGLPGMSGGDLDTVRGLAEQNLA
ALERYEADYIMTACATCGGALHKFYPALIGKRHPELAARIRTIAAKTVDAAQLLQQLGLHPEETGAGERLKITYHDPCHL
RTRNITRQPRELLKGSPGVELAEMEGADKCCGLGGTFNVYHYDTSLKINEGKSAAIEKTGADAVVTGCPGCMMQLSDGLK
QHGNRTRVMHTLEVLARALRK
>Mature_421_residues
MDPLKRVENELKKCVKCGACRAHCPAFAAWQREPAVARGKVAIAQHILKGDIELDEQTYAVMSKCLLCGSCVEKCPNDVP
TDEIVVTARESLARRRGLTTFHKAVGQVIKNRRLMSFGALAAAILGPLFFRKVPQTSGLRLRFPLPFVGNRRHIPQIARK
PFMDRHPEVIQGEPGKPRVVFYVGCMTNFVYTEVGEAALALFRHLGCTVIIPKGQGCCGLPGMSGGDLDTVRGLAEQNLA
ALERYEADYIMTACATCGGALHKFYPALIGKRHPELAARIRTIAAKTVDAAQLLQQLGLHPEETGAGERLKITYHDPCHL
RTRNITRQPRELLKGSPGVELAEMEGADKCCGLGGTFNVYHYDTSLKINEGKSAAIEKTGADAVVTGCPGCMMQLSDGLK
QHGNRTRVMHTLEVLARALRK

Specific function: Electron Transfer Protein; May Also Function As The Membrane Anchor For The Glpab Dimer. [C]

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Loosely Bound To The Cytoplasm Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788576, Length=427, Percent_Identity=26.2295081967213, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786497, Length=240, Percent_Identity=30.4166666666667, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994913, Length=419, Percent_Identity=24.582338902148, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 46033; Mature: 46033

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPLKRVENELKKCVKCGACRAHCPAFAAWQREPAVARGKVAIAQHILKGDIELDEQTYA
CCHHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
VMSKCLLCGSCVEKCPNDVPTDEIVVTARESLARRRGLTTFHKAVGQVIKNRRLMSFGAL
HHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
AAAILGPLFFRKVPQTSGLRLRFPLPFVGNRRHIPQIARKPFMDRHPEVIQGEPGKPRVV
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCHHHCCCCCCCEEE
FYVGCMTNFVYTEVGEAALALFRHLGCTVIIPKGQGCCGLPGMSGGDLDTVRGLAEQNLA
EEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALERYEADYIMTACATCGGALHKFYPALIGKRHPELAARIRTIAAKTVDAAQLLQQLGLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEETGAGERLKITYHDPCHLRTRNITRQPRELLKGSPGVELAEMEGADKCCGLGGTFNVY
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCEEHHCCCCHHHCCCCCCEEEE
HYDTSLKINEGKSAAIEKTGADAVVTGCPGCMMQLSDGLKQHGNRTRVMHTLEVLARALR
EEECEEEECCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
K
C
>Mature Secondary Structure
MDPLKRVENELKKCVKCGACRAHCPAFAAWQREPAVARGKVAIAQHILKGDIELDEQTYA
CCHHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
VMSKCLLCGSCVEKCPNDVPTDEIVVTARESLARRRGLTTFHKAVGQVIKNRRLMSFGAL
HHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
AAAILGPLFFRKVPQTSGLRLRFPLPFVGNRRHIPQIARKPFMDRHPEVIQGEPGKPRVV
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCHHHCCCCCCCEEE
FYVGCMTNFVYTEVGEAALALFRHLGCTVIIPKGQGCCGLPGMSGGDLDTVRGLAEQNLA
EEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALERYEADYIMTACATCGGALHKFYPALIGKRHPELAARIRTIAAKTVDAAQLLQQLGLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEETGAGERLKITYHDPCHLRTRNITRQPRELLKGSPGVELAEMEGADKCCGLGGTFNVY
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCEEHHCCCCHHHCCCCCCEEEE
HYDTSLKINEGKSAAIEKTGADAVVTGCPGCMMQLSDGLKQHGNRTRVMHTLEVLARALR
EEECEEEECCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: FAD; glycerol-3-phosphate [C]

Specific reaction: FAD + glycerol-3-phosphate = FADH2 + dihydroxy-acetone-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]