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The map label for this gene is glcD [H]

Identifier: 39998385

GI number: 39998385

Start: 3615489

End: 3616868

Strand: Direct

Name: glcD [H]

Synonym: GSU3296

Alternate gene names: 39998385

Gene position: 3615489-3616868 (Clockwise)

Preceding gene: 39998384

Following gene: 39998386

Centisome position: 94.79

GC content: 62.46

Gene sequence:

>1380_bases
ATGATTGATTCCCGCATCATTCAAGAAATGAAACTAATCGTCGGCACCGACAACGTGGCGACTGACCGCCAGGATCTGCT
CTGTTACGGCTATGACGCCACCCAGATGGAATTTCTCCCCGACGCCGTGGTCCACCCGGCCTCGCCGGAGGAGATCGCCG
CCATCCTGAAGCTGGCCAATGCGGAGCGCTTTCCGGTCTTTCCTCGGGGCGCCGGCAGCGGCTTCACCGGCGGCGCGCTC
CCCAAGGGGGGCGGCATCGTCCTGGTCGTTACCCGACTGAACCGCATCCTCCGCATCGACACCGAGAACCTGGTCGCCGA
GGTGGAGCCGGGTGTCGTCACGGAGCAGTTCCAACAGGAGGTGGAAAAGCTGGGGCTCTTCTACCCCCCCGATCCGGCCT
CACTCAAGTTTTCCACCTTGGGCGGCAACGTGGCCGAGAACGCCGGCGGCCCCCGCTGCGTCAAGTACGGCGTCACCCGC
GACTTCGTCATGGGTCTGGAAGTGGTGCTTCCCACCGGCGAAATCATCCGCACCGGCGGCGAAACCTACAAGGGAGTGGT
CGGATACGACCTGACCCGCCTCCTGTGCGGCAGCGAGGGAACCCTCGGGGTCATCACCAAGATCATCTTCAAGCTGTTGC
CACTGCCCGAGGCCAAGAAGACCATGCTGACCATCTTCGACTCCATCGACGGTGCGGCAAAGGCGGTCTCCACGATAATC
GGCAACAAGATCATTCCCACGACCCTCGAGTTCATGGACCACGCCACCCTCCAGTGCGTGGAGAAGCGCTTCAATCTGGG
GATTCCGGCAGAGGGGCGGGCGGTGCTCCTGATCGAGGTGGACGGAGACCGGGACCTGATCGAAAAGCAGGCGGCACGCA
TCCAGGACCTGATCAGGCCCCTGGGCCTCGTGGAATGCAAGATCGCCCGGGACGCGGCCGAGTCCGAGGCCCTCTGGAAG
GTGCGGCGCCTGGTGTCGCCATCGTTGCGGGACGTGAATCCGGACAAGTTCAACGAGGACATCGTGGTCCCGCGCTCGAA
GGTACCGGATGTTATCCGGGTCATCGACAAGATCCGGCAGAAGTACGACATTCCCATCGTCAACTTCGGCCATGCGGGCG
ACGGCAACATCCACGTCAACGTCATGATCGACAAGGCTATTCCTGGACAAGAGGAGAAGGCCCACCGGGCCATCGGCGAA
ATCTTCCAGGCCGCCCTGGACCTGAACGGCACCATGTCCGGCGAACACGGCGTTGGCCTGGCCAAGCAACCATACATCCA
TCTGGAGCTGAAACCGGCCCAGGTGGCGGCCATGAAGGCCGTCAAGAAGGCACTGGACCCGAACAACATCCTCAATCCGG
GAAAGATGTTTCCCGCGTGA

Upstream 100 bases:

>100_bases
GAGGGGGGAACGGTGCTGCCGATAATGATGTAGGGGCAAACCTCGTGTTTGCCCGATCTTGGGCGATCACAAGGATCGCC
CCTACAAGGACATTGCAACA

Downstream 100 bases:

>100_bases
TGCGGCGGCAGCCGCCAGGTACGCCGTGAGCGGCTTGCGGGCACAGATTCAGTTCCGCGCACTGCGCGACAAAGAAAGGT
CGTAGATTTGGATCCGCTGA

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MIDSRIIQEMKLIVGTDNVATDRQDLLCYGYDATQMEFLPDAVVHPASPEEIAAILKLANAERFPVFPRGAGSGFTGGAL
PKGGGIVLVVTRLNRILRIDTENLVAEVEPGVVTEQFQQEVEKLGLFYPPDPASLKFSTLGGNVAENAGGPRCVKYGVTR
DFVMGLEVVLPTGEIIRTGGETYKGVVGYDLTRLLCGSEGTLGVITKIIFKLLPLPEAKKTMLTIFDSIDGAAKAVSTII
GNKIIPTTLEFMDHATLQCVEKRFNLGIPAEGRAVLLIEVDGDRDLIEKQAARIQDLIRPLGLVECKIARDAAESEALWK
VRRLVSPSLRDVNPDKFNEDIVVPRSKVPDVIRVIDKIRQKYDIPIVNFGHAGDGNIHVNVMIDKAIPGQEEKAHRAIGE
IFQAALDLNGTMSGEHGVGLAKQPYIHLELKPAQVAAMKAVKKALDPNNILNPGKMFPA

Sequences:

>Translated_459_residues
MIDSRIIQEMKLIVGTDNVATDRQDLLCYGYDATQMEFLPDAVVHPASPEEIAAILKLANAERFPVFPRGAGSGFTGGAL
PKGGGIVLVVTRLNRILRIDTENLVAEVEPGVVTEQFQQEVEKLGLFYPPDPASLKFSTLGGNVAENAGGPRCVKYGVTR
DFVMGLEVVLPTGEIIRTGGETYKGVVGYDLTRLLCGSEGTLGVITKIIFKLLPLPEAKKTMLTIFDSIDGAAKAVSTII
GNKIIPTTLEFMDHATLQCVEKRFNLGIPAEGRAVLLIEVDGDRDLIEKQAARIQDLIRPLGLVECKIARDAAESEALWK
VRRLVSPSLRDVNPDKFNEDIVVPRSKVPDVIRVIDKIRQKYDIPIVNFGHAGDGNIHVNVMIDKAIPGQEEKAHRAIGE
IFQAALDLNGTMSGEHGVGLAKQPYIHLELKPAQVAAMKAVKKALDPNNILNPGKMFPA
>Mature_459_residues
MIDSRIIQEMKLIVGTDNVATDRQDLLCYGYDATQMEFLPDAVVHPASPEEIAAILKLANAERFPVFPRGAGSGFTGGAL
PKGGGIVLVVTRLNRILRIDTENLVAEVEPGVVTEQFQQEVEKLGLFYPPDPASLKFSTLGGNVAENAGGPRCVKYGVTR
DFVMGLEVVLPTGEIIRTGGETYKGVVGYDLTRLLCGSEGTLGVITKIIFKLLPLPEAKKTMLTIFDSIDGAAKAVSTII
GNKIIPTTLEFMDHATLQCVEKRFNLGIPAEGRAVLLIEVDGDRDLIEKQAARIQDLIRPLGLVECKIARDAAESEALWK
VRRLVSPSLRDVNPDKFNEDIVVPRSKVPDVIRVIDKIRQKYDIPIVNFGHAGDGNIHVNVMIDKAIPGQEEKAHRAIGE
IFQAALDLNGTMSGEHGVGLAKQPYIHLELKPAQVAAMKAVKKALDPNNILNPGKMFPA

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=466, Percent_Identity=30.0429184549356, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI37595754, Length=489, Percent_Identity=28.6298568507157, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI119964728, Length=425, Percent_Identity=29.8823529411765, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI4501993, Length=457, Percent_Identity=24.0700218818381, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1789351, Length=431, Percent_Identity=37.5870069605568, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1787977, Length=529, Percent_Identity=27.5992438563327, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI48994907, Length=495, Percent_Identity=24.040404040404, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI48994914, Length=133, Percent_Identity=38.3458646616541, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71992373, Length=460, Percent_Identity=30.6521739130435, Blast_Score=208, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI71992381, Length=460, Percent_Identity=29.5652173913043, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17534361, Length=452, Percent_Identity=27.6548672566372, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71992392, Length=384, Percent_Identity=30.2083333333333, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17556096, Length=220, Percent_Identity=32.2727272727273, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320027, Length=487, Percent_Identity=28.3367556468172, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6320023, Length=452, Percent_Identity=28.9823008849558, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6320764, Length=476, Percent_Identity=27.3109243697479, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI18921117, Length=427, Percent_Identity=32.3185011709602, Blast_Score=208, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24639277, Length=427, Percent_Identity=32.3185011709602, Blast_Score=208, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24639275, Length=427, Percent_Identity=32.3185011709602, Blast_Score=208, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24653753, Length=515, Percent_Identity=23.8834951456311, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 49849; Mature: 49849

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSRIIQEMKLIVGTDNVATDRQDLLCYGYDATQMEFLPDAVVHPASPEEIAAILKLAN
CCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHCCHHHCCCCCHHHHHHHHHHHC
AERFPVFPRGAGSGFTGGALPKGGGIVLVVTRLNRILRIDTENLVAEVEPGVVTEQFQQE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHEEECCHHHHEECCCCCHHHHHHHH
VEKLGLFYPPDPASLKFSTLGGNVAENAGGPRCVKYGVTRDFVMGLEVVLPTGEIIRTGG
HHHCCCCCCCCCCCEEEEECCCCHHHCCCCCEEEEECCCHHHHHCCCEEECCCHHHHCCC
ETYKGVVGYDLTRLLCGSEGTLGVITKIIFKLLPLPEAKKTMLTIFDSIDGAAKAVSTII
CHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHH
GNKIIPTTLEFMDHATLQCVEKRFNLGIPAEGRAVLLIEVDGDRDLIEKQAARIQDLIRP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
LGLVECKIARDAAESEALWKVRRLVSPSLRDVNPDKFNEDIVVPRSKVPDVIRVIDKIRQ
CCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHH
KYDIPIVNFGHAGDGNIHVNVMIDKAIPGQEEKAHRAIGEIFQAALDLNGTMSGEHGVGL
HHCCCEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AKQPYIHLELKPAQVAAMKAVKKALDPNNILNPGKMFPA
CCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIDSRIIQEMKLIVGTDNVATDRQDLLCYGYDATQMEFLPDAVVHPASPEEIAAILKLAN
CCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHCCHHHCCCCCHHHHHHHHHHHC
AERFPVFPRGAGSGFTGGALPKGGGIVLVVTRLNRILRIDTENLVAEVEPGVVTEQFQQE
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHEEECCHHHHEECCCCCHHHHHHHH
VEKLGLFYPPDPASLKFSTLGGNVAENAGGPRCVKYGVTRDFVMGLEVVLPTGEIIRTGG
HHHCCCCCCCCCCCEEEEECCCCHHHCCCCCEEEEECCCHHHHHCCCEEECCCHHHHCCC
ETYKGVVGYDLTRLLCGSEGTLGVITKIIFKLLPLPEAKKTMLTIFDSIDGAAKAVSTII
CHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHH
GNKIIPTTLEFMDHATLQCVEKRFNLGIPAEGRAVLLIEVDGDRDLIEKQAARIQDLIRP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
LGLVECKIARDAAESEALWKVRRLVSPSLRDVNPDKFNEDIVVPRSKVPDVIRVIDKIRQ
CCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHH
KYDIPIVNFGHAGDGNIHVNVMIDKAIPGQEEKAHRAIGEIFQAALDLNGTMSGEHGVGL
HHCCCEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AKQPYIHLELKPAQVAAMKAVKKALDPNNILNPGKMFPA
CCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]