Click here to switch to the map view.

The map label for this gene is pcnB [H]

Identifier: 39998340

GI number: 39998340

Start: 3561440

End: 3562807

Strand: Direct

Name: pcnB [H]

Synonym: GSU3250

Alternate gene names: 39998340

Gene position: 3561440-3562807 (Clockwise)

Preceding gene: 39998324

Following gene: 39998342

Centisome position: 93.37

GC content: 66.96

Gene sequence:

>1368_bases
ATGAGCATCGAACCGGTCATCATTCCCCGCGCCAACCACCCCATCTCCCGCTCCCTCGTCAGCCCCAACGCCCTCCGGGT
CCTCTACCGTCTCCGGGACAACGGCTTTATCGCCTACCTGGTGGGGGGATGCGTCCGGGATCTGCTGCTGGGGCGCGAAC
CCAAGGATTTCGACGTGGCAACCGACGCCACGCCCAACCAGATCAAGCGCATCTTCCGCAACTGCCGGCTGGTGGGGCGC
CGTTTCAGGCTCGCCCACATTCACTTTACCGACGAAATCATCGAGGTGGCCACCTTCCGCGCGTTGTCTCCCCCCGAACA
AGGTGAAGGCGAGCCGGTTGCCGATGAGACTGGACAACGGGGTGGAGATGAAGCGGAACGCCTCCGTCCGCCACGCCACC
TGGTGAGCGACGAGGGAATGGTCCTGCGGGACAATGTTTTCGGAACCCCGGGCGAGGATGCCCTGCGCCGGGATTTCACC
GTCAATGCCCTGGCCTACTGCATCGCCGACTTTTCGATCATCGACTACGCGGGGGGCATGGAAGATCTGCAGCGGGGGAT
GATCCGCACCATCGGCGATCCGCGGGTCCGCTTCACCGAGGACCCGGTCCGGATGCTCCGGGCGGTCAGGTTCGCTGCCG
TGCTGGGGTTCGCCGTGGAGGAGGAGACCTGGCAGGCGATGCTCGATCTGGCACCGGCAATTACCAGGGCGACGGCGCCG
CGGCTTTACGAGGAGATGCTCAAGCTCTTCCTGTCGGGGGAAGGTGAGCGGGCCTATCAGCTCCTGCGGCAGACCGGCCT
CTTCGCGCACCTCTTCCCCGGCTTCGAGGCGTGGCTCGGCGAGGAAACCGACGGGTTCCCCCACGCCCGGACCGGCAGTG
CCCTCGAATGGGTAGACTCACAGGTGGGCAGCGGCGAGCCGGTGTCACCACCGCTCCTCCTCGCGCTCCTCTTTGGCGGG
TACCTGGAAGAACAGTCCGAACGCTTCATGGGCCAGGGGGCTTCTCCCCTGGACGCGGTGAGTGCCGCCGTGGCGGCCTT
TCTGGGTGAGCAGGCGCCGCTGGTGGCAGTGCCGCACCGGATCGGGCTTGCCATGCGCGAGATTCTCGCCCTCCAGCAGC
GACTGCACAGGATACCCGGCAAACGCCCCCAGGCGGTTCTGGCCCGGGCCAGCTTTGCCGAGGCAATGGCCTACCTCCGC
TGCCGTTGCGCCATGAACGGCGAGGACAGCCCGGTTCTCGCGTGGTGGGAGCGCTACGCCCGCGACAGCGTCATGCCTCC
CGTGGAACCCTGTTCAGCCGGAGGCGAGACAAAGAAACCACGGCGGCGTCGCCGGCGGCGCGGCGTCAGGAAGGCGGCTC
CGCAGTGA

Upstream 100 bases:

>100_bases
CCATGGGTGTCATCATAGCCCAAAGGGGGTCTCCATGTCATGACGCAGGTCAAAAGGCGCATTTTTGCTTTTCAAAGGAT
TCTGTGTTATGAATCCCCCC

Downstream 100 bases:

>100_bases
GTCGAGTCTCTTGCAAAACGTTATGAGGACGCCGGGATGCCAGGCGCAAGCGAGCGAGGAAGCGAGGCGTACCCGTCGGT
ACGTTGAGCTTTCGAGTGAG

Product: polyA polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSIEPVIIPRANHPISRSLVSPNALRVLYRLRDNGFIAYLVGGCVRDLLLGREPKDFDVATDATPNQIKRIFRNCRLVGR
RFRLAHIHFTDEIIEVATFRALSPPEQGEGEPVADETGQRGGDEAERLRPPRHLVSDEGMVLRDNVFGTPGEDALRRDFT
VNALAYCIADFSIIDYAGGMEDLQRGMIRTIGDPRVRFTEDPVRMLRAVRFAAVLGFAVEEETWQAMLDLAPAITRATAP
RLYEEMLKLFLSGEGERAYQLLRQTGLFAHLFPGFEAWLGEETDGFPHARTGSALEWVDSQVGSGEPVSPPLLLALLFGG
YLEEQSERFMGQGASPLDAVSAAVAAFLGEQAPLVAVPHRIGLAMREILALQQRLHRIPGKRPQAVLARASFAEAMAYLR
CRCAMNGEDSPVLAWWERYARDSVMPPVEPCSAGGETKKPRRRRRRRGVRKAAPQ

Sequences:

>Translated_455_residues
MSIEPVIIPRANHPISRSLVSPNALRVLYRLRDNGFIAYLVGGCVRDLLLGREPKDFDVATDATPNQIKRIFRNCRLVGR
RFRLAHIHFTDEIIEVATFRALSPPEQGEGEPVADETGQRGGDEAERLRPPRHLVSDEGMVLRDNVFGTPGEDALRRDFT
VNALAYCIADFSIIDYAGGMEDLQRGMIRTIGDPRVRFTEDPVRMLRAVRFAAVLGFAVEEETWQAMLDLAPAITRATAP
RLYEEMLKLFLSGEGERAYQLLRQTGLFAHLFPGFEAWLGEETDGFPHARTGSALEWVDSQVGSGEPVSPPLLLALLFGG
YLEEQSERFMGQGASPLDAVSAAVAAFLGEQAPLVAVPHRIGLAMREILALQQRLHRIPGKRPQAVLARASFAEAMAYLR
CRCAMNGEDSPVLAWWERYARDSVMPPVEPCSAGGETKKPRRRRRRRGVRKAAPQ
>Mature_454_residues
SIEPVIIPRANHPISRSLVSPNALRVLYRLRDNGFIAYLVGGCVRDLLLGREPKDFDVATDATPNQIKRIFRNCRLVGRR
FRLAHIHFTDEIIEVATFRALSPPEQGEGEPVADETGQRGGDEAERLRPPRHLVSDEGMVLRDNVFGTPGEDALRRDFTV
NALAYCIADFSIIDYAGGMEDLQRGMIRTIGDPRVRFTEDPVRMLRAVRFAAVLGFAVEEETWQAMLDLAPAITRATAPR
LYEEMLKLFLSGEGERAYQLLRQTGLFAHLFPGFEAWLGEETDGFPHARTGSALEWVDSQVGSGEPVSPPLLLALLFGGY
LEEQSERFMGQGASPLDAVSAAVAAFLGEQAPLVAVPHRIGLAMREILALQQRLHRIPGKRPQAVLARASFAEAMAYLRC
RCAMNGEDSPVLAWWERYARDSVMPPVEPCSAGGETKKPRRRRRRRGVRKAAPQ

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=414, Percent_Identity=43.719806763285, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1789436, Length=249, Percent_Identity=31.3253012048193, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=213, Percent_Identity=30.9859154929577, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 50635; Mature: 50504

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEPVIIPRANHPISRSLVSPNALRVLYRLRDNGFIAYLVGGCVRDLLLGREPKDFDVA
CCCCCEEEECCCCCHHHHHCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCC
TDATPNQIKRIFRNCRLVGRRFRLAHIHFTDEIIEVATFRALSPPEQGEGEPVADETGQR
CCCCHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCC
GGDEAERLRPPRHLVSDEGMVLRDNVFGTPGEDALRRDFTVNALAYCIADFSIIDYAGGM
CCCHHHHCCCCHHHHCCCCEEEECCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCH
EDLQRGMIRTIGDPRVRFTEDPVRMLRAVRFAAVLGFAVEEETWQAMLDLAPAITRATAP
HHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLYEEMLKLFLSGEGERAYQLLRQTGLFAHLFPGFEAWLGEETDGFPHARTGSALEWVDS
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHHH
QVGSGEPVSPPLLLALLFGGYLEEQSERFMGQGASPLDAVSAAVAAFLGEQAPLVAVPHR
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCHH
IGLAMREILALQQRLHRIPGKRPQAVLARASFAEAMAYLRCRCAMNGEDSPVLAWWERYA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RDSVMPPVEPCSAGGETKKPRRRRRRRGVRKAAPQ
HCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCC
>Mature Secondary Structure 
SIEPVIIPRANHPISRSLVSPNALRVLYRLRDNGFIAYLVGGCVRDLLLGREPKDFDVA
CCCCEEEECCCCCHHHHHCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCC
TDATPNQIKRIFRNCRLVGRRFRLAHIHFTDEIIEVATFRALSPPEQGEGEPVADETGQR
CCCCHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCC
GGDEAERLRPPRHLVSDEGMVLRDNVFGTPGEDALRRDFTVNALAYCIADFSIIDYAGGM
CCCHHHHCCCCHHHHCCCCEEEECCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHCCCH
EDLQRGMIRTIGDPRVRFTEDPVRMLRAVRFAAVLGFAVEEETWQAMLDLAPAITRATAP
HHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLYEEMLKLFLSGEGERAYQLLRQTGLFAHLFPGFEAWLGEETDGFPHARTGSALEWVDS
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHHH
QVGSGEPVSPPLLLALLFGGYLEEQSERFMGQGASPLDAVSAAVAAFLGEQAPLVAVPHR
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECCHH
IGLAMREILALQQRLHRIPGKRPQAVLARASFAEAMAYLRCRCAMNGEDSPVLAWWERYA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RDSVMPPVEPCSAGGETKKPRRRRRRRGVRKAAPQ
HCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]