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The map label for this gene is proA

Identifier: 39998301

GI number: 39998301

Start: 3516342

End: 3517598

Strand: Reverse

Name: proA

Synonym: GSU3211

Alternate gene names: 39998301

Gene position: 3517598-3516342 (Counterclockwise)

Preceding gene: 39998302

Following gene: 39998300

Centisome position: 92.23

GC content: 62.85

Gene sequence:

>1257_bases
ATGTCGATCGCCGAAAAGATTCGAAACATCGCCGCCGACGCCCGCCAGGCCGCAATAGCCATGGCGAAGCTTTCCGCCTC
CGCCAAGAACGAGCTTCTCCTGGCCATGGCCGGTTCTCTGGTCAGGAACACCGTCCAGCTCATTGAGGAAAACCGGAAGG
ACCTGGAAGCGGGCGAGGCCAAGGGGCTTTCGACCGCCATGCTCGACCGGCTCATGCTCAACGAGGCCCGAATCAAGGCC
ATGGCCGACGGCCTACGTGAGGTGGCGGCTCTCTCCGACCCGGTGGGGGAGGTGACGCGGATGTGGACCCGCCCTAACGG
CCTCCGTGTGGGCAAGATGCGGATTCCCTTGGGGGTCATCGGGATAATCTACGAGGCGCGCCCCAACGTCACCGCCGATG
CCGCGGCTCTCTGCCTCAAGAGCGGCAACAGCGTCATTCTGCGGGGCGGCTCCGAAGCGATCCATTCCAATCTGGCTATC
GCCCGCATCCTCGGGGAAGAACTGAAGCGGGCGGGCATCCCCGCTGCGGCCTTGTCGGTCGTTCCCTTCCCCGAGCGCGA
GGGGGTTCTGGAGATGCTCAAGCAGGAGGAGTTCATCGACCTGATCATCCCGCGGGGAGGCGAAAGTCTGATCCGTTTCG
TGGTCGAGAACTCGCGCATCCCGGTCATCAAGCACTACAAGGGGGTCTGCCACGTTTTCGTGGACGCGGATGCCGATTTC
GACATGGCGGAAAAGATCATCGTCAACGGCAAGGTCCAGCGGCCCGGGGTCTGCAATGCCCTGGAGACGCTCCTGGTCCA
CAAGGATATTGCGGAGACCTTTATCCCCCGTATTGCCGAGACCCTCATCGAGCTGAAGGTGGAGCTGAGGGGCGACGACT
GCGTCCGCCAGTTTGTGCCCCAGGCCACAAAGGCCACCGAGGACGACTGGCATGCCGAATACCTGGAGCTGATCCTCGCG
GTCCGGGTGGTGGACGACCTGGACGAGGCCGTGGCCCACATCAACCGTTACGGCTCGCTCCACACCGAGGCCATCGTCAC
CCGCGACTATCACAATGCCCAGCGCTTCATCCGGGAGGTGAACTCCAGCACTGTGCTGGTCAATGCCTCCACCCGATTTG
CCGATGGCAACCAACTGGGGCTCGGTGCCGAGATCGGCATCTCCACCACCAAGCTCCACTCCTTCGGCCCCATGGGACTG
GAGGATCTGACAACGACCAAGTTCATCGTCTATGGCGATGGACAGGTGAGGCCGTGA

Upstream 100 bases:

>100_bases
CGCCGGCCCTCTTCCCACAGAGCCGAACCAAAGGTGACAACGTAGAAATTAGTGTCCTGCCACAGCATCAACAGCTGAAT
CCGCAAGGGAGGGGTTTGAC

Downstream 100 bases:

>100_bases
GCGGGGGCAGGGACCCGGAGGCCGGCAAGGGATGAAAACGGGCATTCTCGGCGGCACGTTCAACCCGGTGCACGTGGCTC
ACCTCCGCATCGCGGAGGAG

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSIAEKIRNIAADARQAAIAMAKLSASAKNELLLAMAGSLVRNTVQLIEENRKDLEAGEAKGLSTAMLDRLMLNEARIKA
MADGLREVAALSDPVGEVTRMWTRPNGLRVGKMRIPLGVIGIIYEARPNVTADAAALCLKSGNSVILRGGSEAIHSNLAI
ARILGEELKRAGIPAAALSVVPFPEREGVLEMLKQEEFIDLIIPRGGESLIRFVVENSRIPVIKHYKGVCHVFVDADADF
DMAEKIIVNGKVQRPGVCNALETLLVHKDIAETFIPRIAETLIELKVELRGDDCVRQFVPQATKATEDDWHAEYLELILA
VRVVDDLDEAVAHINRYGSLHTEAIVTRDYHNAQRFIREVNSSTVLVNASTRFADGNQLGLGAEIGISTTKLHSFGPMGL
EDLTTTKFIVYGDGQVRP

Sequences:

>Translated_418_residues
MSIAEKIRNIAADARQAAIAMAKLSASAKNELLLAMAGSLVRNTVQLIEENRKDLEAGEAKGLSTAMLDRLMLNEARIKA
MADGLREVAALSDPVGEVTRMWTRPNGLRVGKMRIPLGVIGIIYEARPNVTADAAALCLKSGNSVILRGGSEAIHSNLAI
ARILGEELKRAGIPAAALSVVPFPEREGVLEMLKQEEFIDLIIPRGGESLIRFVVENSRIPVIKHYKGVCHVFVDADADF
DMAEKIIVNGKVQRPGVCNALETLLVHKDIAETFIPRIAETLIELKVELRGDDCVRQFVPQATKATEDDWHAEYLELILA
VRVVDDLDEAVAHINRYGSLHTEAIVTRDYHNAQRFIREVNSSTVLVNASTRFADGNQLGLGAEIGISTTKLHSFGPMGL
EDLTTTKFIVYGDGQVRP
>Mature_417_residues
SIAEKIRNIAADARQAAIAMAKLSASAKNELLLAMAGSLVRNTVQLIEENRKDLEAGEAKGLSTAMLDRLMLNEARIKAM
ADGLREVAALSDPVGEVTRMWTRPNGLRVGKMRIPLGVIGIIYEARPNVTADAAALCLKSGNSVILRGGSEAIHSNLAIA
RILGEELKRAGIPAAALSVVPFPEREGVLEMLKQEEFIDLIIPRGGESLIRFVVENSRIPVIKHYKGVCHVFVDADADFD
MAEKIIVNGKVQRPGVCNALETLLVHKDIAETFIPRIAETLIELKVELRGDDCVRQFVPQATKATEDDWHAEYLELILAV
RVVDDLDEAVAHINRYGSLHTEAIVTRDYHNAQRFIREVNSSTVLVNASTRFADGNQLGLGAEIGISTTKLHSFGPMGLE
DLTTTKFIVYGDGQVRP

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=407, Percent_Identity=37.5921375921376, Blast_Score=271, Evalue=8e-73,
Organism=Homo sapiens, GI21361368, Length=407, Percent_Identity=37.5921375921376, Blast_Score=271, Evalue=8e-73,
Organism=Escherichia coli, GI1786438, Length=410, Percent_Identity=48.2926829268293, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17569969, Length=413, Percent_Identity=36.8038740920097, Blast_Score=260, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI17569967, Length=413, Percent_Identity=36.8038740920097, Blast_Score=260, Evalue=9e-70,
Organism=Saccharomyces cerevisiae, GI6324899, Length=431, Percent_Identity=34.8027842227378, Blast_Score=272, Evalue=8e-74,
Organism=Drosophila melanogaster, GI21357643, Length=409, Percent_Identity=38.1418092909535, Blast_Score=264, Evalue=7e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_GEOSL (Q747Q4)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954252.1
- ProteinModelPortal:   Q747Q4
- SMR:   Q747Q4
- GeneID:   2688337
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3211
- NMPDR:   fig|243231.1.peg.3190
- TIGR:   GSU3211
- HOGENOM:   HBG318080
- OMA:   HRIEAMA
- ProtClustDB:   PRK00197
- BioCyc:   GSUL243231:GSU_3211-MONOMER
- BRENDA:   1.2.1.41
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45642; Mature: 45511

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAEKIRNIAADARQAAIAMAKLSASAKNELLLAMAGSLVRNTVQLIEENRKDLEAGEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGLSTAMLDRLMLNEARIKAMADGLREVAALSDPVGEVTRMWTRPNGLRVGKMRIPLGVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCEEE
GIIYEARPNVTADAAALCLKSGNSVILRGGSEAIHSNLAIARILGEELKRAGIPAAALSV
EEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHEE
VPFPEREGVLEMLKQEEFIDLIIPRGGESLIRFVVENSRIPVIKHYKGVCHVFVDADADF
CCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCHHHHCCCEEEEEEECCCCH
DMAEKIIVNGKVQRPGVCNALETLLVHKDIAETFIPRIAETLIELKVELRGDDCVRQFVP
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHCC
QATKATEDDWHAEYLELILAVRVVDDLDEAVAHINRYGSLHTEAIVTRDYHNAQRFIREV
CHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCHHHHHHHHHHC
NSSTVLVNASTRFADGNQLGLGAEIGISTTKLHSFGPMGLEDLTTTKFIVYGDGQVRP
CCCEEEEECCCEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
SIAEKIRNIAADARQAAIAMAKLSASAKNELLLAMAGSLVRNTVQLIEENRKDLEAGEA
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGLSTAMLDRLMLNEARIKAMADGLREVAALSDPVGEVTRMWTRPNGLRVGKMRIPLGVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCEEE
GIIYEARPNVTADAAALCLKSGNSVILRGGSEAIHSNLAIARILGEELKRAGIPAAALSV
EEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHEE
VPFPEREGVLEMLKQEEFIDLIIPRGGESLIRFVVENSRIPVIKHYKGVCHVFVDADADF
CCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCHHHHCCCEEEEEEECCCCH
DMAEKIIVNGKVQRPGVCNALETLLVHKDIAETFIPRIAETLIELKVELRGDDCVRQFVP
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHCC
QATKATEDDWHAEYLELILAVRVVDDLDEAVAHINRYGSLHTEAIVTRDYHNAQRFIREV
CHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCHHHHHHHHHHC
NSSTVLVNASTRFADGNQLGLGAEIGISTTKLHSFGPMGLEDLTTTKFIVYGDGQVRP
CCCEEEEECCCEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA