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The map label for this gene is prfA [H]

Identifier: 39998194

GI number: 39998194

Start: 3407771

End: 3408838

Strand: Reverse

Name: prfA [H]

Synonym: GSU3104

Alternate gene names: 39998194

Gene position: 3408838-3407771 (Counterclockwise)

Preceding gene: 39998195

Following gene: 39998193

Centisome position: 89.37

GC content: 62.36

Gene sequence:

>1068_bases
ATGTTCGAGAAGATAGAAGAACTGGAAGTACGTTACCACGAGCTCGAATCGTTGCTGGCCGACCCCGCGGTGCTTGGCAA
TCAGCCCGAATTCCGCAGGCTGTCCCGGGAGCATAACGACCTGACGCCGCTCATCGAGTCCTATCGCACGTACAAAAAGG
TGCTGGAGGAGATGGAGGGGAACCGGGAGTTGCTGGCCGATCCCGAGATGAAGGAAATGGCCCAGGCCGAGCTGGAGGAA
CTGGAGCAGCGACAGGAGGAACTGGAAGGCGAAATCAAGCTCCTTCTCCTGCCCAGGGATCCAAACGACGACCGCAACGT
AATTCTGGAAATCCGCGCCGGCACCGGCGGCGACGAATCGGCCCTCTTCGCGGGCGACCTGTTCCGGATGTATTCCCGCT
TTGCCGAGCGCAACCGCTGGAAGGTGGAGGTCATGTCCGCCTCCGAGTCGGAGCGGGGGGGCTTCAAGGAGATCGTGGCC
CTCATCGAGGGGCAGGGGGTATTTGCCAAGCTCAAGTACGAATCGGGGACCCACCGGGTACAACGGGTGCCGGAGACCGA
GGCTCAGGGGCGCATCCACACGAGCGCCTGCACCGTTGCGGTTCTGCCCGAGGCCGAAGATATCGAGGTGGACATCAACC
CGGCCGATCTCAAGATCGACGTCTACCGTGCCTCCGGGGCCGGCGGGCAGCACGTCAACAAGACCGAATCGGCAGTCCGG
ATCACCCACATTCCCACCGGGATCGTGGTGGAGTGCCAGGACGAGCGGAGCCAGATCAAGAACCGGGCCAAGGCCATGAA
GGTTCTGAAGACCAAGATCCTCGACGGTCTCCATCAGGAGCAGAACGCACGGATCGCCGCGGACCGGAAACAGCAAGTGG
GAAGCGGCGACCGGAGCGAGCGGATCCGTACCTACAACTTCCCCCAGGGCCGGATGACTGATCACCGGATCGGCCTCACC
CTCTACCGCCTCGACTCTCTCATGGAAGGAGACATCGCCGAGGTGGTGGATGCGCTCCGCACTCATTATCAGATGGAGGC
CCTGAAGGCCCAGGCCGAAGCGGCCTGA

Upstream 100 bases:

>100_bases
CAGGTGGAGGTGGCGATCCGCTCTATGACCGAGGCCCTGGAAGTGAACGACGGCTACCGGGATGATCGTCTGGTGGTGTA
ATCAACAAAGGACGCAGTGA

Downstream 100 bases:

>100_bases
TCCGCGCGGACAGCGGAGGCGGCAGATGACGGAAAAGCCGGAAATCTGGACCATTCGCAAGGTTCTCGACTGGACCAGGG
GCTACCTGGCTGAAAAGGGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MFEKIEELEVRYHELESLLADPAVLGNQPEFRRLSREHNDLTPLIESYRTYKKVLEEMEGNRELLADPEMKEMAQAELEE
LEQRQEELEGEIKLLLLPRDPNDDRNVILEIRAGTGGDESALFAGDLFRMYSRFAERNRWKVEVMSASESERGGFKEIVA
LIEGQGVFAKLKYESGTHRVQRVPETEAQGRIHTSACTVAVLPEAEDIEVDINPADLKIDVYRASGAGGQHVNKTESAVR
ITHIPTGIVVECQDERSQIKNRAKAMKVLKTKILDGLHQEQNARIAADRKQQVGSGDRSERIRTYNFPQGRMTDHRIGLT
LYRLDSLMEGDIAEVVDALRTHYQMEALKAQAEAA

Sequences:

>Translated_355_residues
MFEKIEELEVRYHELESLLADPAVLGNQPEFRRLSREHNDLTPLIESYRTYKKVLEEMEGNRELLADPEMKEMAQAELEE
LEQRQEELEGEIKLLLLPRDPNDDRNVILEIRAGTGGDESALFAGDLFRMYSRFAERNRWKVEVMSASESERGGFKEIVA
LIEGQGVFAKLKYESGTHRVQRVPETEAQGRIHTSACTVAVLPEAEDIEVDINPADLKIDVYRASGAGGQHVNKTESAVR
ITHIPTGIVVECQDERSQIKNRAKAMKVLKTKILDGLHQEQNARIAADRKQQVGSGDRSERIRTYNFPQGRMTDHRIGLT
LYRLDSLMEGDIAEVVDALRTHYQMEALKAQAEAA
>Mature_355_residues
MFEKIEELEVRYHELESLLADPAVLGNQPEFRRLSREHNDLTPLIESYRTYKKVLEEMEGNRELLADPEMKEMAQAELEE
LEQRQEELEGEIKLLLLPRDPNDDRNVILEIRAGTGGDESALFAGDLFRMYSRFAERNRWKVEVMSASESERGGFKEIVA
LIEGQGVFAKLKYESGTHRVQRVPETEAQGRIHTSACTVAVLPEAEDIEVDINPADLKIDVYRASGAGGQHVNKTESAVR
ITHIPTGIVVECQDERSQIKNRAKAMKVLKTKILDGLHQEQNARIAADRKQQVGSGDRSERIRTYNFPQGRMTDHRIGLT
LYRLDSLMEGDIAEVVDALRTHYQMEALKAQAEAA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=283, Percent_Identity=48.4098939929329, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI34577120, Length=337, Percent_Identity=40.3560830860534, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI166795305, Length=178, Percent_Identity=47.1910112359551, Blast_Score=181, Evalue=9e-46,
Organism=Escherichia coli, GI1787462, Length=351, Percent_Identity=58.1196581196581, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=39.4029850746269, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=273, Percent_Identity=39.1941391941392, Blast_Score=191, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=40, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI19921226, Length=258, Percent_Identity=44.5736434108527, Blast_Score=224, Evalue=6e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40283; Mature: 40283

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEKIEELEVRYHELESLLADPAVLGNQPEFRRLSREHNDLTPLIESYRTYKKVLEEMEG
CCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
NRELLADPEMKEMAQAELEELEQRQEELEGEIKLLLLPRDPNDDRNVILEIRAGTGGDES
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCC
ALFAGDLFRMYSRFAERNRWKVEVMSASESERGGFKEIVALIEGQGVFAKLKYESGTHRV
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCCHHH
QRVPETEAQGRIHTSACTVAVLPEAEDIEVDINPADLKIDVYRASGAGGQHVNKTESAVR
HCCCCCHHCCEEEECEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCEEE
ITHIPTGIVVECQDERSQIKNRAKAMKVLKTKILDGLHQEQNARIAADRKQQVGSGDRSE
EEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHCCCCCCCC
RIRTYNFPQGRMTDHRIGLTLYRLDSLMEGDIAEVVDALRTHYQMEALKAQAEAA
CCEEECCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFEKIEELEVRYHELESLLADPAVLGNQPEFRRLSREHNDLTPLIESYRTYKKVLEEMEG
CCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
NRELLADPEMKEMAQAELEELEQRQEELEGEIKLLLLPRDPNDDRNVILEIRAGTGGDES
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCC
ALFAGDLFRMYSRFAERNRWKVEVMSASESERGGFKEIVALIEGQGVFAKLKYESGTHRV
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCCCHHH
QRVPETEAQGRIHTSACTVAVLPEAEDIEVDINPADLKIDVYRASGAGGQHVNKTESAVR
HCCCCCHHCCEEEECEEEEEECCCCCCCEEECCCCCEEEEEEEECCCCCCCCCCCCCEEE
ITHIPTGIVVECQDERSQIKNRAKAMKVLKTKILDGLHQEQNARIAADRKQQVGSGDRSE
EEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHCCCCCCCC
RIRTYNFPQGRMTDHRIGLTLYRLDSLMEGDIAEVVDALRTHYQMEALKAQAEAA
CCEEECCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA