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The map label for this gene is dnaG [H]

Identifier: 39998180

GI number: 39998180

Start: 3396176

End: 3397939

Strand: Reverse

Name: dnaG [H]

Synonym: GSU3090

Alternate gene names: 39998180

Gene position: 3397939-3396176 (Counterclockwise)

Preceding gene: 39998181

Following gene: 39998179

Centisome position: 89.09

GC content: 61.85

Gene sequence:

>1764_bases
ATGTCAAACATCCCCGACGATAAGATTAGGGAGATACGCGAGCGAGCTCCGATTCTGGAGGTGGTCTCGGATTATGTGAG
CCTCCGGAAATCGGGCGCCAATTACCAGGGACTCTGTCCGTTCCACGGCGAGAAGACCCCCTCCTTCAACGTGAATCCGG
CCCGGGGGATCTTTCACTGTTTCGGTTGCGGCGTTGGCGGAAATGCCATCACCTTTGTCATGAAGATGGAGGGACTGTCG
TTTCCCGAAGCGGTCAAGTTCCTGGGCAAGCGGGTCGGTGTCGAAATCGAGGACCGTCCCGTTACCGCCGCAGAGAAACG
GCGGCAGGACGAACGCGAAGAGCTCTATGGGATCATGGACCTGGCTGCCAGGCTCTATCGCGAGGCCCTGGAGCGAAACG
AAGAGGCCGGCGCGGCCCGGCGCTACCTTGAAACGCGCGGTGTGGACCGGGAGACGTCCGAGGCGTACCGTCTCGGGTTC
GCGCCGGACCGGTGGGATTATCTCTCGCGGCACCTGCAACACCGCAAGGTCTCCATGGATCTGGCGGAACGGCTGGGACT
CGTGCGTCGGCGCGAGGGGGGCGGCTACTTCGATATCTTCCGCAACCGTCTCTTGTTTGCCATCACCGATATCCACGGAC
GCACGATCGGGTTTGGCGGCAGAGTCCTGGATGACTCGCTGCCCAAATACATCAACTCTCCCGAGTCGCCCATCTATCAC
AAGAGCGACGTGCTCTTCGGCGTCCACCTGGCAAAGGGCGCTCTGCGGGAGCAGGGCAGCGCCATTATCGTAGAGGGTTA
TTTCGATCATCTTGCCCTCTATCAGGCAGGGGTCCGGAACGTGGTCGCCACTTGCGGCACGGCCTTGACCGAAGGCCATG
TGAAGCTTTTGCGGCGCTACGCCGGGAAAGCCTACACCCTGTTCGATGCGGATAGTGCCGGACGGAAGGCGACCGTGCGG
GCGATCGACCTCTTTCTTGCGGAAAACTTCCCGGCCCACGTGGTGGAGTTGACCCAGGGAGAAGACCCGGACAGTTTCGT
CAAGAAAGAGGGGGCGGACATCTTCCGGACACGGCTCGCCAAGGCCCGGCCGGCATTTGATTTTCTCTTCCGCGACCTGC
TCGGACAGGTGGATGCGGGTACGGTAGAGGGAAAGCTGAAGGTGGCGAACGAGTTGGCGCCCCGCTTGGCAAAGCTCGCC
AATCCCATCGAGCGGGAACTCTACGTGAAGGAGATCGCACGGGTTCTGGGTATTGATGTCCGTTCCCTGCTGCGTGCGTC
GGGCGGAGCAGCCGGATCACGCCCTGACCCCCTTCCCCGCCCGACTGCGGAGCGCCGGCCACGTGGCGCCGGTCCCGAAG
AGATGCTGCTTGCTCTCATGGCGCGATCCGCTGAAGTGGCAGATCGAGTCCGCCGCGGTGAGGCGGCGGGGCTATTCCGT
CCCGAGCTTGCTCCCGTTGCCGATGCCATACTTGAGCGGCACAGCCGGCGGGTGGAGATTGACTGGGGGGAGCTTCTCGA
ACTGGTTGCATCCCCCGAGGAAAAGGGACGCATTGCGGCGTTCCTCGTCAACGACGATCATCTCGCGGACGTTGATGTCC
ATAAGGCCTTTGACCAGTGTCGCCAAGCCCTGGAGCGTGGTTCCCTCAAGCGGCTCAAGGACTTGGCCAGAGAGCTGGCT
GCGGCTGATTCGGATACCCCCCGGTACCGGGAGCTGCTTGCCGAAATAGAGGCCTTGCGAAACAGGAAATCGCAGTTATC
TTAA

Upstream 100 bases:

>100_bases
GCGACGGCGCGGGCGTTCATCGACGCCGGAAAAGGGATGGCCGAGGGGTGGCGCGGAAGCCATGGGTCGGAAGCTCAGAC
CCCGCAAATACGGGCTAAAG

Downstream 100 bases:

>100_bases
AGAGACCATGTGTGCAATGAGGTGAACTTGATGGCCAAGAAAAGCACGGACGAAGTCAAGCAACTCATCGACCTGGGAAA
GGAAAAGGGATTCCTCACCT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MSNIPDDKIREIRERAPILEVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHCFGCGVGGNAITFVMKMEGLS
FPEAVKFLGKRVGVEIEDRPVTAAEKRRQDEREELYGIMDLAARLYREALERNEEAGAARRYLETRGVDRETSEAYRLGF
APDRWDYLSRHLQHRKVSMDLAERLGLVRRREGGGYFDIFRNRLLFAITDIHGRTIGFGGRVLDDSLPKYINSPESPIYH
KSDVLFGVHLAKGALREQGSAIIVEGYFDHLALYQAGVRNVVATCGTALTEGHVKLLRRYAGKAYTLFDADSAGRKATVR
AIDLFLAENFPAHVVELTQGEDPDSFVKKEGADIFRTRLAKARPAFDFLFRDLLGQVDAGTVEGKLKVANELAPRLAKLA
NPIERELYVKEIARVLGIDVRSLLRASGGAAGSRPDPLPRPTAERRPRGAGPEEMLLALMARSAEVADRVRRGEAAGLFR
PELAPVADAILERHSRRVEIDWGELLELVASPEEKGRIAAFLVNDDHLADVDVHKAFDQCRQALERGSLKRLKDLARELA
AADSDTPRYRELLAEIEALRNRKSQLS

Sequences:

>Translated_587_residues
MSNIPDDKIREIRERAPILEVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHCFGCGVGGNAITFVMKMEGLS
FPEAVKFLGKRVGVEIEDRPVTAAEKRRQDEREELYGIMDLAARLYREALERNEEAGAARRYLETRGVDRETSEAYRLGF
APDRWDYLSRHLQHRKVSMDLAERLGLVRRREGGGYFDIFRNRLLFAITDIHGRTIGFGGRVLDDSLPKYINSPESPIYH
KSDVLFGVHLAKGALREQGSAIIVEGYFDHLALYQAGVRNVVATCGTALTEGHVKLLRRYAGKAYTLFDADSAGRKATVR
AIDLFLAENFPAHVVELTQGEDPDSFVKKEGADIFRTRLAKARPAFDFLFRDLLGQVDAGTVEGKLKVANELAPRLAKLA
NPIERELYVKEIARVLGIDVRSLLRASGGAAGSRPDPLPRPTAERRPRGAGPEEMLLALMARSAEVADRVRRGEAAGLFR
PELAPVADAILERHSRRVEIDWGELLELVASPEEKGRIAAFLVNDDHLADVDVHKAFDQCRQALERGSLKRLKDLARELA
AADSDTPRYRELLAEIEALRNRKSQLS
>Mature_586_residues
SNIPDDKIREIRERAPILEVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHCFGCGVGGNAITFVMKMEGLSF
PEAVKFLGKRVGVEIEDRPVTAAEKRRQDEREELYGIMDLAARLYREALERNEEAGAARRYLETRGVDRETSEAYRLGFA
PDRWDYLSRHLQHRKVSMDLAERLGLVRRREGGGYFDIFRNRLLFAITDIHGRTIGFGGRVLDDSLPKYINSPESPIYHK
SDVLFGVHLAKGALREQGSAIIVEGYFDHLALYQAGVRNVVATCGTALTEGHVKLLRRYAGKAYTLFDADSAGRKATVRA
IDLFLAENFPAHVVELTQGEDPDSFVKKEGADIFRTRLAKARPAFDFLFRDLLGQVDAGTVEGKLKVANELAPRLAKLAN
PIERELYVKEIARVLGIDVRSLLRASGGAAGSRPDPLPRPTAERRPRGAGPEEMLLALMARSAEVADRVRRGEAAGLFRP
ELAPVADAILERHSRRVEIDWGELLELVASPEEKGRIAAFLVNDDHLADVDVHKAFDQCRQALERGSLKRLKDLARELAA
ADSDTPRYRELLAEIEALRNRKSQLS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=478, Percent_Identity=34.3096234309623, Blast_Score=286, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65507; Mature: 65375

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPDDKIREIRERAPILEVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHC
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
FGCGVGGNAITFVMKMEGLSFPEAVKFLGKRVGVEIEDRPVTAAEKRRQDEREELYGIMD
HCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHH
LAARLYREALERNEEAGAARRYLETRGVDRETSEAYRLGFAPDRWDYLSRHLQHRKVSMD
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LAERLGLVRRREGGGYFDIFRNRLLFAITDIHGRTIGFGGRVLDDSLPKYINSPESPIYH
HHHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCEECCCCCHHHHHHHHHHCCCCCCCEE
KSDVLFGVHLAKGALREQGSAIIVEGYFDHLALYQAGVRNVVATCGTALTEGHVKLLRRY
CCCCEEHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGKAYTLFDADSAGRKATVRAIDLFLAENFPAHVVELTQGEDPDSFVKKEGADIFRTRLA
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHCCHHHHHHHHH
KARPAFDFLFRDLLGQVDAGTVEGKLKVANELAPRLAKLANPIERELYVKEIARVLGIDV
HCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
RSLLRASGGAAGSRPDPLPRPTAERRPRGAGPEEMLLALMARSAEVADRVRRGEAAGLFR
HHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PELAPVADAILERHSRRVEIDWGELLELVASPEEKGRIAAFLVNDDHLADVDVHKAFDQC
CCHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
RQALERGSLKRLKDLARELAAADSDTPRYRELLAEIEALRNRKSQLS
HHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNIPDDKIREIRERAPILEVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHC
CCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
FGCGVGGNAITFVMKMEGLSFPEAVKFLGKRVGVEIEDRPVTAAEKRRQDEREELYGIMD
HCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHH
LAARLYREALERNEEAGAARRYLETRGVDRETSEAYRLGFAPDRWDYLSRHLQHRKVSMD
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LAERLGLVRRREGGGYFDIFRNRLLFAITDIHGRTIGFGGRVLDDSLPKYINSPESPIYH
HHHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCEECCCCCHHHHHHHHHHCCCCCCCEE
KSDVLFGVHLAKGALREQGSAIIVEGYFDHLALYQAGVRNVVATCGTALTEGHVKLLRRY
CCCCEEHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGKAYTLFDADSAGRKATVRAIDLFLAENFPAHVVELTQGEDPDSFVKKEGADIFRTRLA
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHCCHHHHHHHHH
KARPAFDFLFRDLLGQVDAGTVEGKLKVANELAPRLAKLANPIERELYVKEIARVLGIDV
HCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
RSLLRASGGAAGSRPDPLPRPTAERRPRGAGPEEMLLALMARSAEVADRVRRGEAAGLFR
HHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PELAPVADAILERHSRRVEIDWGELLELVASPEEKGRIAAFLVNDDHLADVDVHKAFDQC
CCHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHH
RQALERGSLKRLKDLARELAAADSDTPRYRELLAEIEALRNRKSQLS
HHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]