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The map label for this gene is pleD [H]

Identifier: 39998060

GI number: 39998060

Start: 3263301

End: 3264248

Strand: Direct

Name: pleD [H]

Synonym: GSU2969

Alternate gene names: 39998060

Gene position: 3263301-3264248 (Clockwise)

Preceding gene: 39998059

Following gene: 39998061

Centisome position: 85.56

GC content: 60.97

Gene sequence:

>948_bases
ATGCCCAACAACGACAGCTTCTACAAGAAGGTCCTCGACAATCTCTACGACGGCATCTACTTTGTGGACGAACAGCGTCG
GATCACCTACTGGAACAAGGGTGCGCAGATGCTGACGGGCTATGGGGAGAAGGAAGTCATCGGCCGAAATTGTCATGACA
TCTTTCTCCACCTCGATTCCAACGGCAACGGCATTTGCTCCGATGCGTGCCCCATCGAAAAGACCATCACCGACGGCCGA
AAACGGGATGAGGAGAGCTTTCTCCACCACAAGGACGGCCACCTGGTTCCGGTCTGCCTCAGGGTGGCTCCGATTCAGGA
TACCGATGGCCAGATGGTGGTGGCAGTCGAGGTGACCGGCGACCACTCCCCGCGCCACGCCCTGCGCCAGCAGCTGGAAA
ATCTCCAGCGCCTCGCCCTCTGCGACCCACTTACCACCATCGCCAACCGCCGCTACGTGGAAATGAGCCTCAACTCGCGC
CTGGAAGAAATGCGACGGTACGGCTGGCACTTCGGCATCCTCTTCCTGGACGTTGACCACTTCAAGCAGATCAACGACAC
CCATGGCCACGACACCGGCGACCGGGTACTCAAGATGGTGGCCAAGACCCTGGTGAACAGCGCCCGCTCTTTCGACGTGG
TTGGCCGCTGGGGGGGCGAGGAGTTCATTGCCGTGCTCGCCAACATATCGCGTCGGCAGCTGCCGCGCATCGCAGACAGG
TTCCGGCACCTGGTTGAACAGTCGAGTTTCCGTCTGGACGATCGGCTGCTCCGGGTCACTGTCTCCGTTGGCGCCACCAT
GTCCCACCTGGATGACACGGTCGAATCACTGGTGCAGCGGGCCGACACGCTCATGTACCGGAGCAAGCAGGCAGGCCGCA
ACTGTGTGAGCATCGGCTTCGGCGAAGAGATGCCGGAAACATCGGCCACCCCGTCCGCGTCACCCTGA

Upstream 100 bases:

>100_bases
TCATCCGCACTGGTAATACGCATACGATTGTGATAGTGTTAGGCGTTTTCAAGTTCTGCCATCATGCGTTATGTCACCGT
GAGCGGGATGCGGAGCGCCC

Downstream 100 bases:

>100_bases
CGCGGGGCACCCGTCCACTACCGACTGCAACGAGGAACCCCATGATAACGCATATCGTATTTTTCAAGCTGACCGACCCT
TCAGCCCCCACCATCGCCGC

Product: sensory box/GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MPNNDSFYKKVLDNLYDGIYFVDEQRRITYWNKGAQMLTGYGEKEVIGRNCHDIFLHLDSNGNGICSDACPIEKTITDGR
KRDEESFLHHKDGHLVPVCLRVAPIQDTDGQMVVAVEVTGDHSPRHALRQQLENLQRLALCDPLTTIANRRYVEMSLNSR
LEEMRRYGWHFGILFLDVDHFKQINDTHGHDTGDRVLKMVAKTLVNSARSFDVVGRWGGEEFIAVLANISRRQLPRIADR
FRHLVEQSSFRLDDRLLRVTVSVGATMSHLDDTVESLVQRADTLMYRSKQAGRNCVSIGFGEEMPETSATPSASP

Sequences:

>Translated_315_residues
MPNNDSFYKKVLDNLYDGIYFVDEQRRITYWNKGAQMLTGYGEKEVIGRNCHDIFLHLDSNGNGICSDACPIEKTITDGR
KRDEESFLHHKDGHLVPVCLRVAPIQDTDGQMVVAVEVTGDHSPRHALRQQLENLQRLALCDPLTTIANRRYVEMSLNSR
LEEMRRYGWHFGILFLDVDHFKQINDTHGHDTGDRVLKMVAKTLVNSARSFDVVGRWGGEEFIAVLANISRRQLPRIADR
FRHLVEQSSFRLDDRLLRVTVSVGATMSHLDDTVESLVQRADTLMYRSKQAGRNCVSIGFGEEMPETSATPSASP
>Mature_314_residues
PNNDSFYKKVLDNLYDGIYFVDEQRRITYWNKGAQMLTGYGEKEVIGRNCHDIFLHLDSNGNGICSDACPIEKTITDGRK
RDEESFLHHKDGHLVPVCLRVAPIQDTDGQMVVAVEVTGDHSPRHALRQQLENLQRLALCDPLTTIANRRYVEMSLNSRL
EEMRRYGWHFGILFLDVDHFKQINDTHGHDTGDRVLKMVAKTLVNSARSFDVVGRWGGEEFIAVLANISRRQLPRIADRF
RHLVEQSSFRLDDRLLRVTVSVGATMSHLDDTVESLVQRADTLMYRSKQAGRNCVSIGFGEEMPETSATPSASP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=36.4161849710983, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788381, Length=294, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=34.5454545454545, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=32.2784810126582, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787262, Length=162, Percent_Identity=34.5679012345679, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=141, Percent_Identity=36.1702127659575, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=32.9479768786127, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=36.144578313253, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787541, Length=314, Percent_Identity=24.203821656051, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=35.3293413173653, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87081974, Length=162, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=32.7683615819209, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=164, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=173, Percent_Identity=30.635838150289, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35818; Mature: 35687

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNNDSFYKKVLDNLYDGIYFVDEQRRITYWNKGAQMLTGYGEKEVIGRNCHDIFLHLDS
CCCCHHHHHHHHHHHHCCEEEEECCCEEEEECCCCHHHHCCCCHHHHCCCCEEEEEEECC
NGNGICSDACPIEKTITDGRKRDEESFLHHKDGHLVPVCLRVAPIQDTDGQMVVAVEVTG
CCCEEECCCCCCHHHHHCCCCCCHHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEEEECC
DHSPRHALRQQLENLQRLALCDPLTTIANRRYVEMSLNSRLEEMRRYGWHFGILFLDVDH
CCCHHHHHHHHHHHHHHHHHHCHHHHHHCCEEEEEHHHHHHHHHHHCCCEEEEEEEEHHH
FKQINDTHGHDTGDRVLKMVAKTLVNSARSFDVVGRWGGEEFIAVLANISRRQLPRIADR
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
FRHLVEQSSFRLDDRLLRVTVSVGATMSHLDDTVESLVQRADTLMYRSKQAGRNCVSIGF
HHHHHHHHCCCHHHHHEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
GEEMPETSATPSASP
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
PNNDSFYKKVLDNLYDGIYFVDEQRRITYWNKGAQMLTGYGEKEVIGRNCHDIFLHLDS
CCCHHHHHHHHHHHHCCEEEEECCCEEEEECCCCHHHHCCCCHHHHCCCCEEEEEEECC
NGNGICSDACPIEKTITDGRKRDEESFLHHKDGHLVPVCLRVAPIQDTDGQMVVAVEVTG
CCCEEECCCCCCHHHHHCCCCCCHHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEEEECC
DHSPRHALRQQLENLQRLALCDPLTTIANRRYVEMSLNSRLEEMRRYGWHFGILFLDVDH
CCCHHHHHHHHHHHHHHHHHHCHHHHHHCCEEEEEHHHHHHHHHHHCCCEEEEEEEEHHH
FKQINDTHGHDTGDRVLKMVAKTLVNSARSFDVVGRWGGEEFIAVLANISRRQLPRIADR
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
FRHLVEQSSFRLDDRLLRVTVSVGATMSHLDDTVESLVQRADTLMYRSKQAGRNCVSIGF
HHHHHHHHCCCHHHHHEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
GEEMPETSATPSASP
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA