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The map label for this gene is ackA-1 [H]

Identifier: 39997801

GI number: 39997801

Start: 2985616

End: 2986881

Strand: Reverse

Name: ackA-1 [H]

Synonym: GSU2707

Alternate gene names: 39997801

Gene position: 2986881-2985616 (Counterclockwise)

Preceding gene: 39997803

Following gene: 39997792

Centisome position: 78.31

GC content: 57.35

Gene sequence:

>1266_bases
ATGATCATACTTGCCCTCAATTGCGGCAGCTCATCGGTGAAGTACCAGCTGTTCGACTGGGAGAGAAAAGAGGTCGTTGC
CAAGGGTATGGTGGAGCGGGTCATTATCGGCGACTCGTTCATCATGCACGAGGTGCCGGGTCGGGAAACGTACCGAAAAG
AGTATGAGTGTCCCGATCACCAGGTGGCCATCGATCTGATCATCAGGACCGTCGTGGATGCAGATCATGGCGTTCTGAAG
GATATCAACGAGATATCCGCGGTCGGTCACCGGGTCGTCCACGGGGGGGAAATGTTCACCCGTTCGGTGCTCATTGACGA
AAAGGTGCTGGACGCGGTCAAGGAGGTCCAGCATCTGGCCCCCCTGCACAACCCCCCCAACATCGCCGGTATCGAGGCCG
CTCAGGCGGTTCTCCCCCACGTTCCCCACGTGGCCATTTTCGATACGGCGTTCCATCAGACCATGCCCGAGCATGCCTAC
CTCTATCCGCTTCCCTACGAGTGGTACGAAAAATACGGTGTGCGTCGCTATGGCTTTCACGGTACGTCCCATCTCTACGT
ATCGAAGCGGGCTGCCGTTCTTCTCGGCAAGACGCCTTCCGAGTGCAACATCATCACCATGCATATCGGCAACGGGGTGT
CCCACTGTGCCATCAAGGGTGGAGTTTCCGTTGATACGAGCATGGGGCTTACCCCCCTCGAAGGTGCCGTCATGGGGACT
CGCTGCGGCGACATCGATCCGGCCATCCCCGCGTTCATGATGCAGAAGGAAAATCTTTCCGCCAAGGAGATCGACAGCAT
CCTCAACAAGAAGAGTGGGGTGCTCGGGGTGACCGGTCGCTTTACGGACCGCCGCGACGTGATCGAACATGCGTCCAATG
GTGATCATCGCTGCAAGGTGGCTCTTGATATCGAGGCCTACCGTCTGAAAAAGTACATCGGGACCTATATGGCCGTGCTG
GGGCGCCTCGACGCCGTGGTCTTCACCGCCGGTGTGGGGGAGATGGGGTGGCCCATTCGTGAGAAAACCATCGAGGGCCT
TGAAGGAATTGGCATCAAGCTTGACCGCGAACGGAACAAGGGGGCCATGACCCGCAAGCGGGAAAGCCTCATCACTACCG
ATGACTCCCCCATCAAGGTGTTCGTCATCCCCACGGATGAAGAGCTCGTCTTTACCGAGGACGTGGTGGCCATTCTCAAC
GGCACCTACACCGATCACATGAATTTCGACTATTCGTTTGCACGGACGGACTTTGTGAGAAAGTAA

Upstream 100 bases:

>100_bases
TTTTTCTGTCCCCATCTGTATCATCTCTCTTAAATCATCTTGAAATTGCTTGTTTTATTCGCGTTGAACAATTTTTATTT
TCGCTCAATCAGGAGGAATG

Downstream 100 bases:

>100_bases
CGATCCGCACAACGGATCCTGAAACGCTGAAGGCCGTCGGGCAGCAGTCCGACGGCCTTTTTGCGTGGATGATGGAGATG
GGGGAGGGCAAAAGGCCGCC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MIILALNCGSSSVKYQLFDWERKEVVAKGMVERVIIGDSFIMHEVPGRETYRKEYECPDHQVAIDLIIRTVVDADHGVLK
DINEISAVGHRVVHGGEMFTRSVLIDEKVLDAVKEVQHLAPLHNPPNIAGIEAAQAVLPHVPHVAIFDTAFHQTMPEHAY
LYPLPYEWYEKYGVRRYGFHGTSHLYVSKRAAVLLGKTPSECNIITMHIGNGVSHCAIKGGVSVDTSMGLTPLEGAVMGT
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVLGVTGRFTDRRDVIEHASNGDHRCKVALDIEAYRLKKYIGTYMAVL
GRLDAVVFTAGVGEMGWPIREKTIEGLEGIGIKLDRERNKGAMTRKRESLITTDDSPIKVFVIPTDEELVFTEDVVAILN
GTYTDHMNFDYSFARTDFVRK

Sequences:

>Translated_421_residues
MIILALNCGSSSVKYQLFDWERKEVVAKGMVERVIIGDSFIMHEVPGRETYRKEYECPDHQVAIDLIIRTVVDADHGVLK
DINEISAVGHRVVHGGEMFTRSVLIDEKVLDAVKEVQHLAPLHNPPNIAGIEAAQAVLPHVPHVAIFDTAFHQTMPEHAY
LYPLPYEWYEKYGVRRYGFHGTSHLYVSKRAAVLLGKTPSECNIITMHIGNGVSHCAIKGGVSVDTSMGLTPLEGAVMGT
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVLGVTGRFTDRRDVIEHASNGDHRCKVALDIEAYRLKKYIGTYMAVL
GRLDAVVFTAGVGEMGWPIREKTIEGLEGIGIKLDRERNKGAMTRKRESLITTDDSPIKVFVIPTDEELVFTEDVVAILN
GTYTDHMNFDYSFARTDFVRK
>Mature_421_residues
MIILALNCGSSSVKYQLFDWERKEVVAKGMVERVIIGDSFIMHEVPGRETYRKEYECPDHQVAIDLIIRTVVDADHGVLK
DINEISAVGHRVVHGGEMFTRSVLIDEKVLDAVKEVQHLAPLHNPPNIAGIEAAQAVLPHVPHVAIFDTAFHQTMPEHAY
LYPLPYEWYEKYGVRRYGFHGTSHLYVSKRAAVLLGKTPSECNIITMHIGNGVSHCAIKGGVSVDTSMGLTPLEGAVMGT
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVLGVTGRFTDRRDVIEHASNGDHRCKVALDIEAYRLKKYIGTYMAVL
GRLDAVVFTAGVGEMGWPIREKTIEGLEGIGIKLDRERNKGAMTRKRESLITTDDSPIKVFVIPTDEELVFTEDVVAILN
GTYTDHMNFDYSFARTDFVRK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=396, Percent_Identity=44.1919191919192, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI145693184, Length=398, Percent_Identity=38.9447236180905, Blast_Score=305, Evalue=5e-84,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 46895; Mature: 46895

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01075 ACETATE_KINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILALNCGSSSVKYQLFDWERKEVVAKGMVERVIIGDSFIMHEVPGRETYRKEYECPDH
CEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHEEECCCEEEECCCCCHHHHHCCCCCCH
QVAIDLIIRTVVDADHGVLKDINEISAVGHRVVHGGEMFTRSVLIDEKVLDAVKEVQHLA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHH
PLHNPPNIAGIEAAQAVLPHVPHVAIFDTAFHQTMPEHAYLYPLPYEWYEKYGVRRYGFH
CCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHCCCCCEEEECCCHHHHHHCCCEEECCC
GTSHLYVSKRAAVLLGKTPSECNIITMHIGNGVSHCAIKGGVSVDTSMGLTPLEGAVMGT
CCCEEEEECCEEEEECCCCCCCCEEEEECCCCCCEEEEECCCEECCCCCCCCCCCCEEEC
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVLGVTGRFTDRRDVIEHASNGDHRCKV
CCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCCEEEE
ALDIEAYRLKKYIGTYMAVLGRLDAVVFTAGVGEMGWPIREKTIEGLEGIGIKLDRERNK
EEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHCCCCCCCEEEECCCCC
GAMTRKRESLITTDDSPIKVFVIPTDEELVFTEDVVAILNGTYTDHMNFDYSFARTDFVR
CCHHHHHHHEEECCCCCEEEEEEECCCCEEEHHHHHHHHCCCEECCCCCCEEHHHHHHCC
K
C
>Mature Secondary Structure
MIILALNCGSSSVKYQLFDWERKEVVAKGMVERVIIGDSFIMHEVPGRETYRKEYECPDH
CEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHEEECCCEEEECCCCCHHHHHCCCCCCH
QVAIDLIIRTVVDADHGVLKDINEISAVGHRVVHGGEMFTRSVLIDEKVLDAVKEVQHLA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHH
PLHNPPNIAGIEAAQAVLPHVPHVAIFDTAFHQTMPEHAYLYPLPYEWYEKYGVRRYGFH
CCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHCCCCCEEEECCCHHHHHHCCCEEECCC
GTSHLYVSKRAAVLLGKTPSECNIITMHIGNGVSHCAIKGGVSVDTSMGLTPLEGAVMGT
CCCEEEEECCEEEEECCCCCCCCEEEEECCCCCCEEEEECCCEECCCCCCCCCCCCEEEC
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVLGVTGRFTDRRDVIEHASNGDHRCKV
CCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCCEEEE
ALDIEAYRLKKYIGTYMAVLGRLDAVVFTAGVGEMGWPIREKTIEGLEGIGIKLDRERNK
EEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHCCCCCCCEEEECCCCC
GAMTRKRESLITTDDSPIKVFVIPTDEELVFTEDVVAILNGTYTDHMNFDYSFARTDFVR
CCHHHHHHHEEECCCCCEEEEEEECCCCEEEHHHHHHHHCCCEECCCCCCEEHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA